Assessment of Mold-Specific Volatile Organic Compounds and Molds Using Sorbent Tubes and a CDC/NIOSH-Developed Tool in Homes Affected by Hurricane Ian
Source abstract
Abstract
Flooding from hurricanes creates damp indoor environments that support mold growth and microbial contamination, posing long-term health risks for occupants. This pilot study evaluated TMVOCs, microbial activity, and environmental conditions in 13 Hurricane Ian-affected residences across multiple flood-affected neighborhoods. Air samples were collected using sorbent tubes and analyzed by gas chromatography–mass spectrometry, while microbial activity on surfaces was assessed via ATP bioluminescence. Visible mold and dampness were documented with the CDC/NIOSH Dampness and Mold Assessment Tool, and environmental measurements included temperature, relative humidity, and surface as well as hidden moisture. Median (IQR) TMVOC concentrations were 12 (8) µg/m3, with 61% of homes exceeding the 10 µg/m3 benchmark set by previous researchers despite minimal visible contamination. Spearman’s correlation revealed significant negative relationships between odor and surface microbial activity (ρ = −0.569, p < 0.05), indicating that organic debris may play a more crucial role in microbial activity within the tested homes, and that odors might originate from hidden microbes instead of surface microbial growth. Our study emphasizes the necessity of utilizing both chemical (TMVOC) and biological (ATP) indicators to evaluate poor air quality caused by molds in flood-affected homes, serving as a supplement to routine visible mold assessments.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Indoor Air Quality and Microbial Exposure · Advanced Chemical Sensor Technologies · Conservation Techniques and Studies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Biostatistics, Epidemiology, and Environmental Health Sciences, Jiann-Ping Hsu College of Public Health, Georgia Southern University, Statesboro, GA 30460, USA
James H. Oliver, Jr., Institute for Coastal Plain Science, Georgia Southern University, Statesboro, GA 30460, USA
School of Earth, Environment & Sustainability, College of Science and Mathematics, Georgia Southern University, Statesboro, GA 30460, USA
Department of Health Policy and Community Health, Jiann-Ping Hsu College of Public Health, Georgia Southern University, Statesboro, GA 30460, USA
Department of Marine & Earth Sciences, The Water School, Florida Gulf Coast University, Fort Myers, FL 33965, USA
Department of Health Sciences, Marieb College of Health & Human Services, Florida Gulf Coast University, Fort Myers, FL 33965, USA
Funding
Faculty Research Committee · Georgia Southern University
References (35)
Show source-provided references
1. Mold prevention strategies and possible health effects in the aftermath of hurricanes and major floods
2. Public health and economic impact of dampness and mold10.1111/j.1600-0668.2007.00474.x ↗
3. Respiratory and allergic health effects of dampness, mold, and dampness-related agents: A review of the epidemiologic evidence10.1289/ehp.1002410 ↗
4. Quansah, R., Jaakkola, M.S., Hugg, T.T., Heikkinen, S.A., and Jaakkola, J.J. (2012). Residential dampness and molds and the risk of developing asthma: A systematic review and meta-analysis. PLoS ONE, 7.10.1371/journal.pone.0047526 ↗
5. Mold and endotoxin levels in the aftermath of Hurricane Katrina: A pilot project of homes in New Orleans undergoing renovation10.1289/ehp.9258 ↗
6. Aerosolization of fungi, (1-->3)-beta-D glucan, and endotoxin from flood-affected materials collected in New Orleans homes10.1016/j.envres.2008.12.010 ↗
7. Exposure matrices of endotoxin, (1→3)-β-d-glucan, fungi, and dust mite allergens in flood-affected homes of New Orleans10.1016/j.scitotenv.2010.07.087 ↗
8. Increased prevalence of indoor Aspergillus and Penicillium species is associated with indoor flooding and coastal proximity: A case study of 28 moldy buildings10.1039/D1EM00202C ↗
9. Correlation between ERMI values and other moisture and mold assessments of homes in the American Healthy Homes Survey
10. Microbial volatile organic compounds10.1080/10408440802291497 ↗
11. The relationship between exposure to microbial volatile organic compound and allergy prevalence in single-family homes10.1016/j.scitotenv.2012.02.026 ↗
12. Mechanisms of Activation of the Sensory Irritant Receptor by Airborne Chemicals10.3109/10408449109089879 ↗
13. Haines, S.R., Hall, E.C., Marciniak, K., Misztal, P.K., Goldstein, A.H., Adams, R.I., and Dannemiller, K.C. (2021). Microbial growth and volatile organic compound (VOC) emissions from carpet and drywall under elevated relative humidity conditions. Microbiome, 9.10.1186/s40168-021-01158-y ↗
14. Indoor molds, bacteria, microbial volatile organic compounds and plasticizers in schools--associations with asthma and respiratory symptoms in pupils10.1111/j.1600-0668.2006.00466.x ↗
15. Hospodsky, D., Qian, J., Nazaroff, W.W., Yamamoto, N., Bibby, K., Rismani-Yazdi, H., and Peccia, J. (2012). Human occupancy as a source of indoor airborne bacteria. PLoS ONE, 7.10.1371/journal.pone.0034867 ↗
16. An evaluation of hospital cleaning regimes and standards10.1053/jhin.1999.0717 ↗
17. Floor cleaning: Effect on bacteria and organic materials in hospital rooms10.1016/j.jhin.2008.09.014 ↗
18. National Hurricane Center (2025, September 15). Tropical Cyclone Report: Hurricane Ian. National Oceanic and Atmospheric Administration, Available online: https://www.nhc.noaa.gov/data/tcr/AL092022_Ian.pdf.
19. NIOSH (2025, September 15). Dampness and Mold Assessment Tool for General Buildings—Form & Instructions. Cox-Ganser J, Martin M, Park JH, Game, S. Morgantown WV: U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, DHHS (NIOSH) Publication No. 2019-115. Available online: https://doi.org/10.26616/NIOSHPUB2019115.10.26616/NIOSHPUB2019115 ↗
20. EPA (2025, September 25). Compendium Method TO-17: Determination of Volatile Organic Compounds in Ambient Air Using Active Sampling onto Sorbent Tubes. U.S. Environmental Protection Agency. Method TO-17—Determination of Volatile Organic Compounds in Ambient Air Using Active Sampling onto Sorbent Tubes, Available online: https://www.epa.gov/sites/default/files/2019-11/documents/to-17r.pdf.
21. (2004). Indoor Air—Part 6: Determination of Organic Compounds (VVOC, VOC, SVOC) in Indoor and Test Chamber Air by Active Sampling on Sorbent Tubes, Thermal Desorption, and Gas Chromatography Using MS or MSFID (Standard No. ISO 16000-6).
22. Volatile compounds originating from mixed microbial cultures on building materials under various humidity conditions10.1128/AEM.64.8.2914-2919.1998 ↗
23. Volatile organic compounds (VOCs) in homes are associated with asthma and lung function among adults in Northern Europe10.1016/j.envpol.2023.121103 ↗
24. Volatile metabolites from mold growth on building materials and synthetic media10.1016/S0045-6535(99)00273-8 ↗
25. Microbial volatile organic compounds in the air of moldy and mold-free indoor environments10.1111/j.1600-0668.2007.00513.x ↗
26. Dominant microbial volatile organic compounds in 23 U.S. homes10.1016/j.chemosphere.2012.06.066 ↗
27. mVOC 4.0: A database of microbial volatiles10.1093/nar/gkae961 ↗
28. Detection of microbial volatile organic compounds (MVOCs) produced by moulds on various materials10.1078/1438-4639-00094 ↗
29. Betancourt, D.A., Krebs, K., Moore, S.A., and Martin, S.M. (2013). Microbial volatile organic compound emissions from Stachybotrys chartarum growing on gypsum wallboard and ceiling tile. BMC Microbiol., 13.10.1186/1471-2180-13-283 ↗
30. Size-resolved emission rates of airborne bacteria and fungi in an occupied classroom10.1111/j.1600-0668.2012.00769.x ↗
31. Longitudinal analysis of microbial interaction between humans and the indoor environment10.1126/science.1254529 ↗
32. Miller, J.D., Flannigan, B., Samson, R.A., and Cox-Ganser, D.O. (2011). Microbial Growth in Indoor Environments. Microorganisms in Home and Indoor Work Environments, CRC Press. [2nd ed.].
33. World Health Organization (2009). WHO Guidelines for Indoor Air Quality: Dampness and Mould, WHO Regional Office for Europe.
34. Institute of Medicine (US) Committee on Damp Indoor Spaces and Health (2004). Damp Indoor Spaces and Health, The National Academies Press.
35. World Health Organization (WHO) (1990). Bioaerosols: Assessment and Control, World Health Organization.