journal article·2025-09-20Open access

Advanced Spectroscopic and Thermoanalytical Quantification of LLDPE in Mealworm Frass: A Multitechnique Approach

Encarnación Martínez-Sabater, Rosa Peñalver, Margarita Ros, José A. Pascual, Raul Moral, Frutos C. Marhuenda-Egea

Applied Sciences· Volume 15· Issue 18

Abstract

Plastic pollution from polyethylene-based materials is a critical environmental concern due to their high persistence. Here, we report the first proof-of-concept application of a multitechnique analytical framework for quantifying linear low-density polyethylene (LLDPE) in Tenebrio molitor frass. Artificially enriched frass–LLDPE mixtures were analyzed using thermogravimetric analysis (TGA), TGA coupled with Fourier-Transform Infrared Spectroscopy (FTIR) and Mass Spectrometry (MS), TGA under inert atmosphere, and solid-state 13C nuclear magnetic resonance spectroscopy with Cross-Polarization and Magic Angle Spinning (CP-MAS NMR) 13C CP-MAS NMR combined with interval Partial Least Squares (iPLS) modeling. Thermal methods provided insight into decomposition pathways but showed reduced specificity at <1% w/w due to matrix interference. CP-MAS NMR offered matrix-independent quantification, with characteristic signals in the 10–45 ppm region and a calculated LOD and LOQ of 0.173% and 0.525% w/w, respectively. The LOQ lies within the reported ingestion range for T. molitor (0.8–3.2% w/w in frass), confirming biological relevance. This validated workflow establishes CP-MAS NMR as the most robust tool for quantifying polyethylene residues in complex matrices and provides a foundation for in vivo biodegradation studies and environmental monitoring.
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Research context

OpenAlex topics: thermodynamics and calorimetric analyses · Chemical Thermodynamics and Molecular Structure · Mass Spectrometry Techniques and Applications

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Affiliations

GIAAMA Research Group, Centro de Investigación e Innovación Agroalimentaria y Agroambiental (CIAGRO-UMH), Agrochemistry and Environment Department, Miguel Hernández University, 03312 Orihuela, Alicante, Spain

Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, 30100 Murcia, Murcia, Spain

Department of Soil and Water Conservation and Organic Waste Management, Centro de Edafología y Biología Aplicada del Segura, CEBAS-CSIC, P.O. Box 4195, 30080 Murcia, Murcia, Spain

Department of Biochemistry and Molecular Biology and Agricultural Chemistry and Edafology, University of Alicante, Carretera San Vicente del Raspeig s/n, 03690 Alicante, Alicante, Spain

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