Enhanced Sensitivity of 17-α-Ethinylestradiol (EE2) Detection Using Carbon Quantum Dots-Integrated Tapered Optical Fiber
Source abstract
Abstract
In this study, we developed a tapered optical fiber sensor enhanced with carbon quantum dots (CQDs) for the detection of 17-α-ethinylestradiol (EE2). The sensor operates on the changes in refractive index induced by the interaction between EE2 and antibodies on its surface. The incorporation of CQDs significantly increased the available surface area for receptor–analyte interactions, leading to enhanced sensor performance. The sensor demonstrated high sensitivity of 2.4925 nm/(ng/L) within a detection range of 1 to 10 ng/L, with a strong correlation coefficient (R2 = 0.998). A detection limit as low as 0.0426 ng/L (0.144 pM) was achieved, along with a low dissociation constant of 2.19 × 10−11 M as determined by the Langmuir isotherm model. These findings highlight the potential of the CQD-functionalized optical fiber sensors as a promising tool for sensitive and selective EE2 detection in environmental monitoring applications.
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Research context
OpenAlex topics: Carbon and Quantum Dots Applications · Electrochemical sensors and biosensors · Advanced biosensing and bioanalysis techniques
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Faculty of Electrical & Electronics Engineering Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Pekan 26600, Pahang, Malaysia
Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
International Institute of Aquaculture and Aquatic Sciences, Universiti Putra Malaysia, Port Dickson 71050, Negeri Sembilan, Malaysia
Wireless and Photonics Networks Research Centre, Faculty of Engineering, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
Physics Department, Faculty of Science, Universiti Teknologi Malaysia, Skudai 81310, Johor Bahru, Malaysia
Department of Electrical Engineering, College of Engineering, King Saud University, P.O. Box 800, Riyadh 11421, Saudi Arabia
Department of Engineering, University of Cambridge, Cambridge CB3 0FA, UK
Institute of Nanoscience and Nanotechnology (ION2), Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
Funding
Ministry of Higher Education Malaysia · King Saud University, Kingdom of Saudi Arabia
References (62)
Show source-provided references
1. Toward Plasmonic Neural Probes: SERS Detection of Neurotransmitters through Gold-Nanoislands-Decorated Tapered Optical Fibers with Sub-10 nm Gaps10.1002/adma.202200902 ↗
2. Tunable Nanoislands Decorated Tapered Optical Fibers Reveal Concurrent Contributions in Through-Fiber SERS Detection10.1021/acsphotonics.4c00912 ↗
3. Polokhin, A.A., Shaman, Y.P., Itrin, P.A., Panyaev, I.S., Sysa, A.A., Selishchev, S.V., Kitsyuk, E.P., Pavlov, A.A., and Gerasimenko, A.Y. (2023). Tapered Optical Fiber Sensor Coated with Single-Walled Carbon Nanotubes for Dye Sensing Application. Micromachines, 14.10.3390/mi14030579 ↗
4. Graphene- and Multi-Walled Carbon Nanotubes-Coated Tapered Plastic Optical Fiber for Detection of Lard Adulteration in Olive Oil10.1007/s13320-022-0652-y ↗
5. Liu, J., Li, R., and Yang, B. (2022, November 01). Carbon Dots: A New Type of Carbon-Based Nanomaterial with Wide Applications. Available online: https://pubs.acs.org/doi/pdf/10.1021/acscentsci.0c01306?ref=article_openPDF.
6. Khalaf, A.L., Abdallah, A., and Shabaneh, A. (2019). Carbon Nanotubes and Graphene Oxide Applications in Optochemical Sensors, Elsevier Inc.10.1016/B978-0-12-815757-2.00010-3 ↗
7. Carbon Dot-functionalized Interferometric Optical Fiber Sensor for Detection of Ferric Ions in Biological Samples10.1021/acsami.9b08934 ↗
8. Abd Rani, U., Ng, L.Y., Ng, C.Y., and Mahmoudi, E. (2020). A Review of Carbon Quantum Dots and Their Applications in Wastewater Treatment, Elsevier.10.1016/j.cis.2020.102124 ↗
9. Omar, N.A.S., Fen, Y.W., Irmawati, R., Hashim, H.S., Ramdzan, N.S.M., and Fauzi, N.I.M. (2022). A Review on Carbon Dots: Synthesis, Characterization and Its Application in Optical Sensor for Environmental Monitoring. Nanomaterials, 12.10.3390/nano12142365 ↗
10. Synthesis and Characterization of Fluorescent Carbon Dots from Tapioca10.1002/slct.201900836 ↗
11. Sciortino, A., Cannizzo, A., and Messina, F. (2018). Carbon Nanodots: A Review—From the Current Understanding of the Fundamental Photophysics to the Full Control of the Optical Response. C, 4.10.3390/c4040067 ↗
12. Solvatochromism in highly luminescent environmental friendly carbon quantum dots for sensing applications: Conversion of bio-waste into bio-asset10.1016/j.saa.2017.10.054 ↗
13. Polymeric carbon quantum dots as efficient chlorophyll sensor-analysis based on experimental and computational investigation10.1016/j.optlastec.2023.110259 ↗
14. Carbon quantum dots functionalized tapered optical fiber for highly sensitive and specific detection of Leptospira DNA10.1016/j.optlastec.2022.108696 ↗
15. Zainuddin, N.H., Chee, H.Y., Ahmad, M.Z., Mahdi, M.A., Bakar, M.H.A., and Yaacob, M.H. (2018). Sensitive Leptospira DNA detection using tapered optical fiber sensor. J. Biophotonics, 11.10.1002/jbio.201700363 ↗
16. Biological effects and bioaccumulation of steroidal and phenolic endocrine disrupting chemicals in high-back crucian carp exposed to wastewater treatment plant effluents10.1016/j.envpol.2011.11.036 ↗
17. Capture-SELEX of DNA Aptamers for Estradiol Specifically and Estrogenic Compounds Collectively10.1021/acs.est.2c05808 ↗
18. Impact of Long-Term Exposure to 17A-Ethinylestradiol in the Live-Bearing Fish Heterandria formosa10.1007/s00244-019-00600-5 ↗
19. Predicted-no-effect concentrations for the steroid estrogens estrone, 17β-estradiol, estriol, and 17α-ethinylestradiol10.1002/etc.1825 ↗
20. Monitoring of the 17 EU Watch List contaminants of emerging concern in the Ave and the Sousa Rivers10.1016/j.scitotenv.2018.08.309 ↗
21. 17α-Ethinylestradiol (EE2): Concentrations in the environment and methods for wastewater treatment—An update
22. Occurrence of endocrine disrupting compounds in aqueous environment and their bacterial degradation: A review10.1080/10643389.2015.1061881 ↗
23. Development of ELISA methodologies for the direct determination of 17 b-estradiol and 17 a-ethinylestradiol in complex aqueous matrices10.1016/j.jenvman.2013.03.041 ↗
24. A survey of 17α-ethinylestradiol and mestranol residues in Hawkesbury River, Australia, using a highly specific enzyme-linked immunosorbent assay (ELISA) demonstrates the levels of potential biological significance10.1016/j.ecoenv.2017.06.077 ↗
25. Direct sub-ppt detection of the endocrine disruptor ethinylestradiol in water with a chemiluminescence enzyme-linked immunosorbent assay10.1016/j.aca.2005.07.018 ↗
26. A novel enzyme-linked immunosorbent assay for ethynylestradiol using a long-chain biotinylated EE2 derivative10.1016/j.steroids.2004.01.003 ↗
27. Development of an online SPE-UHPLC-MS/MS method for the multiresidue analysis of the 17 compounds from the EU B Watch list10.1007/s00216-018-1069-8 ↗
28. Determination of estrogen hormones in raw and treated water samples by reverse phase ultra-fast liquid chromatography mass spectrometry—A case study in Johannesburg South, South Africa
29. Liquid chromatography–time-of-flight high-resolution mass spectrometry study and determination of the dansylated products of estrogens and their hydroxylated metabolites in water and wastewater10.1007/s00216-018-1412-0 ↗
30. Immunosensor based on porous gold and reduced graphene platform for the determination of EE2 by electrochemical impedance spectroscopy10.1016/j.jelechem.2021.115604 ↗
31. Ultrasensitive environmental assessment of xeno-estrogens in water samples using label-free graphene immunosensors10.1016/j.ab.2018.02.027 ↗
32. Ultrasensitive biosensor based on polyvinylpyrrolidone/chitosan/reduced graphene oxide electrospun nanofibers for 17α -ethinylestradiol electrochemical detection10.1016/j.apsusc.2018.07.035 ↗
33. Label-free Dengue E protein detection using a functionalized tapered optical fiber sensor10.1016/j.snb.2017.11.005 ↗
34. Fabrication, characterization and response surface method optimization for quantum efficiency of fluorescent nitrogen-doped carbon dots obtained from carboxymethylcellulose of oil palms empty fruit bunch10.1016/j.cjche.2019.04.003 ↗
35. Dengue e Protein Detection Using a Graphene Oxide Integrated Tapered Optical Fiber Sensor
36. Mustapha Kamil, Y., Al-Rekabi, S.H., Yaacob, M.H., Syahir, A., Chee, H.Y., Mahdi, M.A., and Abu Bakar, M.H. (2019). Detection of dengue using PAMAM dendrimer integrated tapered optical fiber sensor. Sci. Rep., 9.10.1038/s41598-019-49891-7 ↗
37. Self-functionalized ultrastable water suspension of luminescent carbon quantum dots10.1016/j.matchemphys.2018.12.031 ↗
38. Synthesis and characterization of high efficient photoluminescent sunlight driven photocatalyst of N-Carbon Quantum Dots10.1016/j.jlumin.2018.04.061 ↗
39. Engineering triangular carbon quantum dots with unprecedented narrow bandwidth emission for multicolored LEDs10.1038/s41467-018-04635-5 ↗
40. Carbon quantum dots: Synthesis, properties and applications10.1039/C4TC00988F ↗
41. Photoluminescence mechanism of carbon dots: Triggering high-color-purity red fluorescence emission through edge amino protonation10.1038/s41467-021-27071-4 ↗
42. Controllable synthesis of biosourced blue-green fluorescent carbon dots from camphor for the detection of heavy metal ions in water10.1039/C4RA10471D ↗
43. Synthesis of photoluminescent carbon dots and its effect on chondrocytes for knee joint therapy applications10.1080/21691401.2019.1593855 ↗
44. Blue-fluorescent and biocompatible carbon dots derived from abundant low-quality coals10.1016/j.jphotobiol.2019.04.004 ↗
45. Sensitive Detection of Goat αs1-Casein Using Tapered Optical Fiber Sensor10.1109/JSTQE.2020.3045131 ↗
46. Raman, Brillouin, and nuclear magnetic resonance spectroscopic studies on shocked borosilicate glass10.1063/1.3592346 ↗
47. Raman spectra of soda-lime-silicate glass doped with rare earth10.1016/j.physb.2011.07.014 ↗
48. Graphene-enhanced Raman spectroscopy of thymine adsorbed on single-layer graphene10.1186/s11671-015-0869-4 ↗
49. The Laser-Raman Spectrum of Ferrocene
50. Role of vibrational spectroscopy in stem cell research10.1155/2012/513286 ↗
51. Variable range hopping and relaxation mechanism in graphene oxide sheets containing sp3 hybridization induced localization10.1007/s10854-018-9003-6 ↗
52. Surface roughness and the sensitivity of D-shaped optical fibre sensors10.1080/09500340.2019.1610521 ↗
53. Effects of surface roughness on optical properties and sensitivity of fiber-optic evanescent wave sensors10.1364/AO.52.003937 ↗
54. Influence of diamond-like carbon overlay properties on refractive index sensitivity of nano-coated optical fibres
55. Detection of 17-α-Ethinylestradiol with Bio-Functionalized Tapered Optical Fiber Sensor10.1016/j.measurement.2024.115305 ↗
56. Highly selective SPR-based fiber optic sensor for the detection of hydrogen peroxide10.1016/j.snb.2020.129062 ↗
57. Electrochemical quantification of 17 α–Ethinylestradiol in biological samples using a Au/Fe3O4 @ TA/MWNT/GCE sensor10.1016/j.matchemphys.2020.122687 ↗
58. Reduced Graphene Oxide-based Sensor for 17α-Ehinylestradiol Voltammetric Determination in Wastewater, Tablets and Synthetic Urine Samples10.1002/elan.202100500 ↗
59. Total Determination of Estrogenic Phenolic Compounds in River Water Using a Sensor Based on Reduced Graphene Oxide and Molecularly Imprinted Polymer10.1002/elan.201800238 ↗
60. Efficient electrochemical biosensors for ethynylestradiol based on the laccase enzyme supported on single-walled carbon nanotubes decorated with nanocrystalline carbon quantum dots10.1039/C6AY02178F ↗
61. Novel Electrochemical Paper-Based Immunocapture Assay for the Quantitative Determination of Ethinylestradiol in Water Samples10.1021/acs.analchem.8b00028 ↗
62. Omar, N.A., Fen, Y.W., Abdullah, J., Mustapha Kamil, Y., Daniyal, W.M., Sadrolhosseini, A.R., and Mahdi, M.A. (2020). Sensitive Detection of Dengue Virus Type 2 E-Proteins Signals Using Self-Assembled Monolayers/Reduced Graphene Oxide-PAMAM Dendrimer Thin Film-SPR Optical Sensor. Sci. Rep., 10.10.1038/s41598-020-59388-3 ↗