Bactericidal activity of clove basil essential oil microemulsion on Aeromonas hydrophila

In this experiment, clove basil essential oil was prepared in microemulsion and its bactericidal activity was evaluated on Aeromonas hydrophila, a common bacterial species that causes hemorrhagic disease in catfish. The results demonstrated that the prepared microemulsion was an oil-in-water type, characterised by transparency, a particle size of 40.61 nm with a low PDI of 0.23. Furthermore, the clove basil essential oil microemulsion had bactericidal activity on A. hydrophila with a minimum inhibitory concentration (MIC) of 1.25 % (v/v). Notably, the microemulsion form of clove basil essential oil displayed improved antibacterial properties compared to its original counterpart. This showed the potential application of clove basil essential oil microemulsion to prevent bacterial diseases in catfish, especially the disease caused by A. hydrophila.