Heart Rate Monitoring System for Fish Larvae Using Interframe Luminance Difference

Danionella, a transparent freshwater species belonging to the Cyprinidae family, has emerged as a valuable model organism in biological and medical research due to its optical transparency. The cardiovascular system of Danionella larvae provides a unique opportunity for non-invasive heart rate monitoring in aquatic animals. Traditional approaches for evaluating larval heart rate often require manual or semi-automated definition of the cardiac region in video recordings. In this study, we developed a simplified heart rate monitoring system that estimates heartbeat activity by analyzing interframe luminance differences in video sequences of Danionella larvae. Our system successfully measured heart rates in the range of 150–155 beats per minute (bpm), consistent with previous findings reporting rates between 140 and 200 bpm. The non-invasive nature of this method offers significant advantages for high-throughput screening and long-term physiological monitoring. Furthermore, this system has potential applications in evaluating environmental stressors, supporting survival and health assessments, and guiding habitat management strategies to ensure stable populations of adult fish in both natural and laboratory settings.