journal article·2025-04-01Open access

Assessment of Pyrethrin Novel Green Extraction Methods from Dalmatian Pyrethrum (Tanacetum cinerariifolium)

Jasna Maršić-Lučić, Stela Jokić, Maja Molnar, Martina Jakovljević Kovač, Marija Banožić, Jerko Hrabar, Ivona Mladineo

Applied Sciences· Volume 15· Issue 7

Abstract

Six novel green extraction techniques were evaluated and optimized to extract pyrethrin from dried Dalmatian pyrethrum (Tanacetum cinerariifolium (Trevir./Sch.Bip.). This approach offers a promising natural alternative to conventional chemotherapeutics. Four methods are presented for the first time in this study: microwave-assisted extraction (MAE), high-voltage electric discharge (HVED) extraction, subcritical water extraction (SWE), and deep eutectic solvent (DES) extraction, together with supercritical CO2 extraction (SC-CO2) and ultrasound-assisted extraction (UAE), for pyrethrin extraction from Dalmatian pyrethrum. The study revealed that supercritical CO2 extraction was the most effective method for extracting all six pyrethrins, yielding the highest total amount of 124.37 ng/mg. This approach offers a “natural” insecticide produced with a clean, environmentally friendly technology that can contribute to the development of sustainable and effective insecticide strategies that are in line with environmental safety and organic production standards. In addition, this research highlights the potential application of pyrethrins as antiparasitic agents, emphasizing their role in environmentally friendly and ecological practices.
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Research context

OpenAlex topics: Phytochemicals and Antioxidant Activities · Essential Oils and Antimicrobial Activity · Plant biochemistry and biosynthesis

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institute of Oceanography and Fisheries, Šetalište I. Meštrovića 63, 21000 Split, Croatia

Faculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 18, 31000 Osijek, Croatia

Faculty of Agriculture and Food Technology, University of Mostar, Biskupa Čule bb, 88000 Mostar, Bosnia and Herzegovina

Institute of Parasitology, Biological Centre of Czech Academy of Sciences, Branišovská 31, 37005 České Budějovice, Czech Republic

Funding

European Regional Development Fund

References (32)

Show source-provided references
1. Pyrethrum an organic and natural pesticide
2. Performance of pyrethroids as domestic insecticides
3. Effects of pyrethroid insecticides on aquatic organisms, Synthetic pyrethroids: Occurrence and behavior in aquatic environments10.1021/bk-2008-0991.ch014 ↗
4. Pyrethroid pesticide residues in the global environment: An overview10.1016/j.chemosphere.2017.10.115 ↗
5. Pyrethric acid of natural pyrethrin insecticide: Complete pathway elucidation and reconstitution in Nicotiana benthamiana10.1111/nph.15821 ↗
6. Pyrethroids influence on fish
7. Morphological and biochemical diversity of Dalmatian pyrethrum (Tanacetum cinerariifolium (Trevir.) Sch. Bip.)
8. Effect of drying conditions on pyrethrins content10.1016/j.indcrop.2005.01.007 ↗
9. Pyrethrum (Tanacetum cinerariifolium) from the northern Adriatic as a potential source of natural insecticide
10. Chemical diversity of the natural populations of Dalmatian Pyrethrum (Tanacetum cinerariifolium (TREVIR.) SCH. BIP.) in Croatia10.1002/cbdv.201200015 ↗
11. Dalmatian Pyrethrum (Tanacetum cinerariifolium/Trevir. /Sch. Bip.)
12. Golden age of insecticide research: Past, present, or future?10.1146/annurev.ento.43.1.1 ↗
13. Separation and quantitative analysis of natural pyrethrins by high-performance liquid chromatography10.1007/BF02493668 ↗
14. Matrix solid-phase dispersion optimization for determination of pyrethrin content in Dalmatian pyrethrum (Tanacetum cinerariifolium/Trevir./Sch. Bip.) by liquid chromatography10.1016/j.indcrop.2019.111999 ↗
15. Comparison of pyrethrins extraction methods efficiencies
16. The extraction efficiency of maceration, UAE and MSPD in the extraction of pyrethrins from Dalmatian pyrethrum
17. Ultrasonic/Soxhlet/supercritical fluid extraction kinetics of pyrethrins from flowers and allethrin from paper strips10.1007/s002160051644 ↗
18. Pyrethrin extraction from pyrethrum flowers using carbon dioxide10.1016/S0896-8446(02)00165-1 ↗
19. Comparative extraction and enrichment techniques for pyrethrins from flowers of Chrysanthemum cinerariaefolium10.1016/j.indcrop.2015.07.043 ↗
20. Preparative supercritical fluid extraction of pyrethrin I and II from pyrethrum flower10.1016/0039-9140(95)01657-0 ↗
21. Supercritical fluid extraction of pyrethrins from pyrethrum flowers (Chrysanthemum cinerariifolium) compared to traditional maceration and cyclic pressurization extraction10.1016/j.supflu.2016.09.012 ↗
22. New perspective in extraction of plant biologically active compounds by green solvents10.1016/j.fbp.2018.03.001 ↗
23. Green extraction of natural products: Concept and principles10.3390/ijms13078615 ↗
24. Recent insights into green extraction techniques as efficient methods for the extraction of bioactive components and essential oils from foods10.1080/19476337.2022.2157492 ↗
25. Barišić, V., Jozinović, A., Flanjak, I., Šubarić, D., Babić, J., Miličević, B., Doko, K., and Ačkar, D. (2020). Difficulties with use of cocoa bean shell in food production and high voltage electrical discharge as a possible solution. Sustainability, 12.10.3390/su12103981 ↗
26. Choline chloride based deep eutectic solvents as a tuneable medium for synthesis of coumarinyl 1, 2, 4-triazoles: Effect of solvent type and temperature10.1002/ejoc.201900249 ↗
27. Subcritical water extraction laboratory plant design and application
28. Supercritical CO2 extraction—A new perspective in the utilisation of food industry by-products
29. Combination of microwave-assisted extraction and ultrasonic-assisted dispersive liquid-liquid microextraction for separation and enrichment of pyrethroids residues in Litchi fruit prior to HPLC determination10.1016/j.foodchem.2017.08.061 ↗
30. Microwave-assisted extractions of active ingredients from plants10.1016/j.chroma.2011.07.040 ↗
31. Recent extraction techniques for natural products: Microwave-assisted extraction and pressurised solvent extraction10.1002/pca.631 ↗
32. Centers for Disease Control and Prevention (CDC), U.S. Department of Health and Human Services (2003). Chemical and Physical Information in Toxicological Profile for Pyrethrins and Pyrethroids, Public Health Service Agency for Toxic Substances and Disease Registry.