Effect of Scenedesmus deserticola JD052 Extracts on Hair Inductivity by Regulating the AKT and GSK3β/β-Catenin Signaling Pathways in Human Dermal Papilla Cells
Source abstract
Abstract
The extract of Scenedesmus deserticola JD052 has been reported to exhibit anti-aging effects on the skin, with research indicating an increase in loliolide, a major active component, through heterotrophic cultivation. In this study, we evaluated the effects of extracts obtained from both photoautotrophic (PE) and heterotrophic (HE) cultures on hair-inductive properties in human dermal papilla (HDP) cells. Biochemical assays demonstrated that both extracts enhanced HDP cell viability and increased the size of three-dimensional dermal papilla (DP) spheres. Notably, the activation of β-catenin, a crucial marker associated with hair growth, was assessed using a luciferase reporter assay, revealing that HE exhibited a significantly higher efficacy than PE. Further analyses indicated that HE promoted the translocation of β-catenin into the nucleus through the phosphorylation and activation of AKT, which also elevated the expression levels of DP signature genes and hair-growth-related autocrine factors. Additionally, conditioned media from HE-treated HDP cells enhanced keratinocyte migration and increased the expression of growth factors, including VEGF and IGF-1. HPLC-MS analysis showed no significant difference in loliolide content; however, specific peaks in HE were identified as pheophorbide A and linolelaidic acid. Thus, HE may enhance hair growth inductivity via AKT/β-catenin signaling.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Hair Growth and Disorders · Silk-based biomaterials and applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Cosmetics Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea
References (100+)
Show source-provided references
1. Al Aboud, A.M., and Zito, P.M. (2024). Alopecia. StatPearls, StatPearls Publishing.
2. Finasteride for hair loss: A review10.1080/09546634.2021.1959506 ↗
3. Minoxidil and its use in hair disorders: A review10.2147/DDDT.S214907 ↗
4. Olumniant (Baricitinib) oral tablets: An insight into FDA-approved systemic treatment for Alopecia Areata10.1016/j.amsu.2022.104157 ↗
5. Baricitinib for the Treatment of Alopecia Areata10.1007/s40265-023-01873-w ↗
6. Finasteride and sexual side effects10.4103/2229-5178.93496 ↗
7. Minoxidil use in dermatology, side effects and recent patents10.2174/187221312800166859 ↗
8. Natural products for male androgenetic alopecia10.1111/dth.15323 ↗
9. Herbal Medicines For Treatment of Androgenic Alopecia
10. Advances in microalgal research and engineering development10.1016/j.copbio.2019.05.013 ↗
11. Mass cultivation and harvesting of microalgal biomass: Current trends and future perspectives10.1016/j.biortech.2021.126406 ↗
12. Microalgae: Therapeutic potentials and applications10.1007/s11033-021-06422-w ↗
13. Exploring the potential of using algae in cosmetics10.1016/j.biortech.2014.12.001 ↗
14. Potential of microalgal compounds in trending natural cosmetics: A review10.1016/j.scp.2020.100304 ↗
15. Microalgae: A multifaceted catalyst for sustainable solutions in renewable energy, food security, and environmental management10.1186/s12934-024-02588-7 ↗
16. Taxonomy and phylogeny of Scenedesmus
17. Microalgal and cyanobacterial cell extracts for use as natural antibacterial additives against food pathogens10.1111/j.1365-2621.2011.02567.x ↗
18. Protective effects of an extract of the freshwater microalga Scenedesmus rubescens on UV-irradiated skin cells10.1111/ics.12450 ↗
19. Zaharieva, M.M., Zheleva-Dimitrova, D., Rusinova-Videva, S., Ilieva, Y., Brachkova, A., Balabanova, V., Gevrenova, R., Kim, T.C., Kaleva, M., and Georgieva, A. (2022). Antimicrobial and Antioxidant Potential of Scenedesmus obliquus Microalgae in the Context of Integral Biorefinery Concept. Molecules, 27.10.3390/molecules27020519 ↗
20. Antimicrobial and anticancer activities of Scenedesmus obliquus metabolites10.1016/j.heliyon.2019.e01404 ↗
21. Identification of Loliolide with Anti-Aging Properties from Scenedesmus deserticola JD05210.4014/jmb.2304.04044 ↗
22. The human hair: From anatomy to physiology10.1111/ijd.12362 ↗
23. The hair follicle as a dynamic miniorgan10.1016/j.cub.2008.12.005 ↗
24. Molecular Mechanisms of Hair Growth and Regeneration: Current Understanding and Novel Paradigms10.1159/000506155 ↗
25. Sonic hedgehog signaling is essential for hair development10.1016/S0960-9822(98)70443-9 ↗
26. Competitive balance of intrabulge BMP/Wnt signaling reveals a robust gene network ruling stem cell homeostasis and cyclic activation10.1073/pnas.1121312110 ↗
27. Molecular principles of hair follicle induction and morphogenesis10.1002/bies.20184 ↗
28. Choi, B.Y. (2020). Targeting Wnt/β-Catenin Pathway for Developing Therapies for Hair Loss. Int. J. Mol. Sci., 21.10.3390/ijms21144915 ↗
29. Wang, N., Zhang, W.-d., Zhong, Z.-y., Zhou, X.-B., Shi, X.-r., and Wang, X. (J. Integr. Agric., 2023). FGF7 secreted from dermal papillae cell regulates the proliferation and differentiation of hair follicle stem cell1, J. Integr. Agric., in press.10.1016/j.jia.2023.10.012 ↗
30. Igf-I signalling controls the hair growth cycle and the differentiation of hair shafts10.1111/j.0022-202X.2005.23946.x ↗
31. Synergistic effect of PDGF and FGF2 for cell proliferation and hair inductive activity in murine vibrissal dermal papilla in vitro10.1016/j.jdermsci.2015.04.007 ↗
32. The Modulatable Stem Cell Niche: Tissue Interactions During Hair and Feather Follicle Regeneration10.1016/j.jmb.2015.07.009 ↗
33. Vascular endothelial growth factor is an autocrine growth factor for hair dermal papilla cells10.1111/1523-1747.ep12326964 ↗
34. Oliva, A.K., Bejaoui, M., Hirano, A., Arimura, T., Linh, T.N., Uchiage, E., Nukaga, S., Tominaga, K., Nozaki, H., and Isoda, H. (2022). Elucidation of the Potential Hair Growth-Promoting Effect of Botryococcus terribilis, Its Novel Compound Methylated-Meijicoccene, and C32 Botryococcene on Cultured Hair Follicle Dermal Papilla Cells Using DNA Microarray Gene Expression Analysis. Biomedicines, 10.10.3390/biomedicines10051186 ↗
35. Zeng, Z., Wang, B., Ibrar, M., Ying, M., Li, S., and Yang, X. (2023). Schizochytrium sp. Extracted Lipids Prevent Alopecia by Enhancing Antioxidation and Inhibiting Ferroptosis of Dermal Papilla Cells. Antioxidants, 12.10.3390/antiox12071332 ↗
36. Comparison of Saccharina japonica-Undaria pinnatifida Mixture and Minoxidil on Hair Growth Promoting Effect in Mice10.5999/aps.2016.43.6.498 ↗
37. Zhao, D., Jiang, Y., Ning, C., Meng, J., Lin, S., Ding, W., and Tao, J. (2014). Transcriptome sequencing of a chimaera reveals coordinated expression of anthocyanin biosynthetic genes mediating yellow formation in herbaceous peony (Paeonia lactiflora Pall). BMC Genom., 15.10.1186/1471-2164-15-689 ↗
38. Water Extract of Mixed Mushroom Mycelia Grown on a Solid Barley Medium Is Protective against Experimental Focal Cerebral Ischemia10.3390/cimb43010030 ↗
39. Cho, D.-H., Yun, J.-H., Choi, D.-Y., Heo, J., Kim, E.K., Ha, J., Yoo, C., Choi, H.I., Lee, Y.J., and Kim, H.-S. (2024). Long-term acclimation to organic carbon enhances the production of loliolide from Scenedesmus deserticola. Bioresour. Technol., 412.10.1016/j.biortech.2024.131408 ↗
40. Yun, H.-S., Kim, Y.-S., and Yoon, H.-S. (2021). Effect of Different Cultivation Modes (Photoautotrophic, Mixotrophic, and Heterotrophic) on the Growth of Chlorella sp. and Biocompositions. Front. Bioeng. Biotechnol., 9.10.3389/fbioe.2021.774143 ↗
41. Dermal papilla cell number specifies hair size, shape and cycling and its reduction causes follicular decline10.1242/dev.090662 ↗
42. Possible mechanisms of miniaturization during androgenetic alopecia or pattern hair loss10.1067/mjd.2001.117428 ↗
43. Follicular miniaturization in female pattern hair loss: Clinicopathological correlations10.1111/j.1365-2133.2006.07409.x ↗
44. Growth Factors in Hair Organ Development and the Hair Growth Cycle10.1016/S0733-8635(05)70384-3 ↗
45. Hair-inducing ability of human dermal papilla cells cultured under Wnt/β-catenin signalling activation10.1111/j.1600-0625.2012.01458.x ↗
46. Park, S., Han, N., Lee, J.-M., Lee, J.-H., and Bae, S. (2023). Effects of Allium hookeri Extracts on Hair-Inductive and Anti-Oxidative Properties in Human Dermal Papilla Cells. Plants, 12.10.3390/plants12091919 ↗
47. Expression dynamics of lymphoid enhancer-binding factor 1 in terminal Schwann cells, dermal papilla, and interfollicular epidermis10.1002/dvdy.562 ↗
48. A β-catenin-driven switch in TCF/LEF transcription factor binding to DNA target sites promotes commitment of mammalian nephron progenitor cells10.7554/eLife.64444 ↗
49. Heterotrophic cultures of microalgae: Metabolism and potential products10.1016/j.watres.2010.08.037 ↗
50. WNT signals are required for the initiation of hair follicle development10.1016/S1534-5807(02)00167-3 ↗
51. Wnt/β-catenin signaling in dermal condensates is required for hair follicle formation10.1016/j.ydbio.2013.11.023 ↗
52. Tcf-1 promotes genomic instability and T cell transformation in response to aberrant β-catenin activation10.1073/pnas.2201493119 ↗
53. Wnt5a attenuates Wnt/β-catenin signalling in human dermal papilla cells10.1111/exd.12101 ↗
54. Activation of β-catenin by carcinogenic Helicobacter pylori10.1073/pnas.0504927102 ↗
55. Kim, J., Shin, J.Y., Choi, Y.-H., Lee, S.Y., Jin, M.H., Kim, C.D., Kang, N.-G., and Lee, S. (2021). Adenosine and Cordycepin Accelerate Tissue Remodeling Process through Adenosine Receptor Mediated Wnt/β-Catenin Pathway Stimulation by Regulating GSK3b Activity. Int. J. Mol. Sci., 22.10.3390/ijms22115571 ↗
56. GSK3: A multifaceted kinase in Wnt signaling10.1016/j.tibs.2009.10.002 ↗
57. Premise and peril of Wnt signaling activation through GSK-3β inhibition10.1016/j.isci.2022.104159 ↗
58. A chiral microenvironment promotes retinal progenitor cell proliferation by activating the Akt and ERK pathways10.1039/D2BM00886F ↗
59. PI3K/Akt signaling pathway is essential for de novo hair follicle regeneration10.1186/s13287-020-01650-6 ↗
60. Amphiregulin promotes hair regeneration of skin-derived precursors via the PI3K and MAPK pathways10.1111/cpr.13106 ↗
61. EB2 promotes hepatocellular carcinoma proliferation and metastasis via MAPK/ERK pathway by modulating microtubule dynamics10.1042/CS20201500 ↗
62. Effects of the PI3K/Akt signaling pathway on the hair inductivity of human dermal papilla cells in hair beads10.1016/j.jbiosc.2022.03.010 ↗
63. Bioactive small molecule enhances skin burn wound healing and hair follicle regeneration by activating PI3K/AKT signaling pathway: A preclinical evaluation in animal model10.1166/jbn.2022.3251 ↗
64. Effect of sinapic acid on hair growth promoting in human hair follicle dermal papilla cells via Akt activation10.1007/s00403-017-1732-5 ↗
65. sPLA2-IIA overexpression in mice epidermis depletes hair follicle stem cells and induces differentiation mediated through enhanced JNK/c-Jun activation10.1002/stem.2418 ↗
66. Monoterpenoid Loliolide Regulates Hair Follicle Inductivity of Human Dermal Papilla Cells by Activating the Akt/β-Catenin Signaling Pathway10.4014/jmb.1908.08018 ↗
67. Narrow-band red LED light promotes mouse hair growth through paracrine growth factors from dermal papilla10.1016/j.jdermsci.2011.09.004 ↗
68. A role of placental growth factor in hair growth10.1016/j.jdermsci.2014.01.011 ↗
69. Restoration of the intrinsic properties of human dermal papilla in vitro
70. Shh maintains dermal papilla identity and hair morphogenesis via a Noggin–Shh regulatory loop10.1101/gad.187401.112 ↗
71. Selective activation of the versican promoter by epithelial– mesenchymal interactions during hair follicle development10.1073/pnas.96.13.7336 ↗
72. Maintaining Hair Inductivity in Human Dermal Papilla Cells: A Review of Effective Methods10.1159/000510152 ↗
73. Dermal papilla-induced hair differentiation of adult epithelial stem cells from human skin10.1152/physiolgenomics.00134.2004 ↗
74. Molecular control of epithelial–mesenchymal interactions during hair follicle cycling10.1046/j.1523-1747.2003.12171.x ↗
75. Mesenchymal–epithelial interactions during hair follicle morphogenesis and cycling10.1016/j.semcdb.2012.08.011 ↗
76. Decorin promotes proliferation and migration of ORS keratinocytes and maintains hair anagen in mice10.1111/exd.13770 ↗
77. Restoration of hair-inductive activity of cultured human follicular keratinocytes by co-culturing with dermal papilla cells10.1016/j.bbrc.2018.09.125 ↗
78. Hair follicle dermal papilla cells at a glance10.1242/jcs.082446 ↗
79. Human hair follicle dermal sheath and papilla cells support keratinocyte growth in monolayer coculture10.1111/exd.12107 ↗
80. Antiviral and anti-inflammatory metabolites from the soft coral Sinularia capillosa10.1021/np9008078 ↗
81. Antioxidant activity and cell protective effect of loliolide isolated from Sargassum ringgoldianum subsp. coreanum10.4490/algae.2011.26.2.201 ↗
82. Yun, J.H., Cho, D.H., Lee, B., Kim, H.S., and Chang, Y.K. (2018). Application of biosurfactant from Bacillus subtilis C9 for controlling cladoceran grazers in algal cultivation systems. Sci. Rep., 8.10.1038/s41598-018-23535-8 ↗
83. Androgenetic alopecia
84. Pathogenesis of Androgenetic Alopecia10.4103/cdr.cdr_29_21 ↗
85. Androgenetic alopecia: An update10.1016/j.jdin.2023.07.005 ↗
86. Jin, M., Chen, Y.-L., He, X., Hou, Y., Chan, Z., and Zeng, R. (2020). Amelioration of Androgenetic Alopecia by Algal Oligosaccharides Prepared by Deep-Sea Bacterium Biodegradation. Front. Microbiol., 11.10.3389/fmicb.2020.567060 ↗
87. Complementary and Alternative Treatments for Alopecia: A Comprehensive Review10.1159/000492035 ↗
88. Hair-Loss Preventing Effect of Grateloupia elliptica10.4062/biomolther.2012.20.1.118 ↗
89. A comparison of photoautotrophic, heterotrophic, and mixotrophic growth for biomass production by the green alga Scenedesmus sp. (Chlorophyceae)10.2216/17-82.1 ↗
90. Heterotrophic and mixotrophic cultivation of microalgae for biodiesel production in agricultural wastewaters and associated challenges—A critical review10.1007/s10811-014-0459-3 ↗
91. Producing liquid transportation fuels from heterotrophic microalgae10.1016/j.apenergy.2012.10.067 ↗
92. Sphere Formation Increases the Ability of Cultured Human Dermal Papilla Cells to Induce Hair Follicles from Mouse Epidermal Cells in a Reconstitution Assay10.1038/jid.2011.250 ↗
93. Hes1 regulates anagen initiation and hair follicle regeneration through modulation of hedgehog signaling10.1002/stem.3117 ↗
94. Boehmite enhances hair follicle growth via stimulation of dermal papilla cells by upregulating β-catenin signalling10.1111/exd.14051 ↗
95. 25742 Evaluation of the active ingredient complex of an anti-hair loss shampoo on the activation of Wnt/betacatenin pathway in human hair follicle dermal papilla cells10.1016/j.jaad.2021.06.297 ↗
96. Ginsenoside Rg4 Enhances the Inductive Effects of Human Dermal Papilla Spheres on Hair Growth Via the AKT/GSK-3β/β-Catenin Signaling Pathway10.4014/jmb.2101.01032 ↗
97. Androgen receptor–mediated paracrine signaling induces regression of blood vessels in the dermal papilla in androgenetic alopecia10.1016/j.jid.2022.01.003 ↗
98. Keratinocyte Growth Factor/Fibroblast Growth Factor-7-regulated Cell Migration and Invasion through Activation of NF-ĸba;B Transcription Factors10.1074/jbc.M606878200 ↗
99. Epidermal Growth Factor and Insulin-Like Growth Factor I Enhance Keratinocyte Migration10.1111/1523-1747.ep12472297 ↗
100. The roles of growth factors in keratinocyte migration10.1089/wound.2014.0540 ↗