journal article·2025-01-01Open access

Acute Myotonometric Changes in the Masseter and Upper Trapezius Muscles After Upper Body Quarter Stretching and Coordination Exercises or Chewing

Marša Magdič, Miloš Kalc, Matjaž Vogrin

Applied Sciences· Volume 15· Issue 1

Abstract

Pathologies in neck and masticatory muscles affect muscle tone and biomechanical and viscoelastic properties, necessitating precise assessment for treatment. This study evaluated the impact of two guided interventions—relaxing exercises targeting the neck and masticatory muscles (“Exercise”) and heavy chewing using six chewing gums (“Chewing”)—on the masseter and upper trapezius muscles. Twelve participants (aged 19–40 years) underwent myotonometric assessment pre- and post-intervention, measuring tone, stiffness, decrement, relaxation time, and creep. The results showed significant changes in the masseter muscle after exercise, with increased stiffness (14.46%, p < 0.001) and tone (7.03%, p < 0.001) but decreased creep (−9.71%, p < 0.001) and relaxation time (−11.36%, p < 0.001). Conversely, chewing decreased stiffness (−8.82%, p < 0.001) and tone (−5.53%, p < 0.001), while it increased creep (9.68%, p < 0.001) and relaxation time (9.98%, p < 0.001). In the trapezius muscles, tone decreased after both interventions (Exercise: −7.65%, p < 0.001; Chewing: −1.06%, p = 0.003), while relaxation increased (Exercise: 1.78%, p < 0.001; Chewing: 2.82%, p < 0.001). These findings reveal the distinct effects of exercise and chewing on muscle properties, emphasising the complexity of their therapeutic potential and the need for further investigation.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Transcranial Magnetic Stimulation Studies · Temporomandibular Joint Disorders · Pain Mechanisms and Treatments

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institute of Sports Medicine, Faculty of Medicine, University of Maribor, 2000 Maribor, Slovenia

Institute for Kinesiology Research, Science and Research Centre Koper, 6000 Koper, Slovenia

Department of Orthopaedics, University Medical Centre Maribor, 2000 Maribor, Slovenia

Funding

Human Resources Development in Sport 2016–2022, Olympic Committee Slovenia

References (67)

Show source-provided references
1. Jaw Exercises in the Treatment of Temporomandibular Disorders—An International Modified Delphi Study10.11607/ofph.2359 ↗
2. Shimada, A., Ogawa, T., Sammour, S.R., Narihara, T., Kinomura, S., Koide, R., Noma, N., and Sasaki, K. (2023). Effectiveness of Exercise Therapy on Pain Relief and Jaw Mobility in Patients with Pain-Related Temporomandibular Disorders: A Systematic Review. Front. Oral. Health, 4.10.3389/froh.2023.1170966 ↗
3. The Face of Dental Sleep Medicine in the 21st Century10.1111/joor.13075 ↗
4. International Consensus on the Assessment of Bruxism: Report of a Work in Progress10.1111/joor.12663 ↗
5. Review of Aetiological Concepts of Temporomandibular Pain Disorders: Towards a Biopsychosocial Model for Integration of Physical Disorder Factors with Psychological and Psychosocial Illness Impact Factors10.1016/j.ejpain.2005.01.012 ↗
6. Zieliński, G., Matysik-Woźniak, A., Baszczowski, M., Rapa, M., Ginszt, M., Pająk, B., Szkutnik, J., Rejdak, R., and Gawda, P. (2023). Myopia & Painful Muscle Form of Temporomandibular Disorders: Connections between Vision, Masticatory and Cervical Muscles Activity and Sensitivity and Sleep Quality. Sci. Rep., 13.10.1038/s41598-023-47550-6 ↗
7. Gałczyńska-Rusin, M., Pobudek-Radzikowska, M., Gawriołek, K., and Czajka-Jakubowska, A. (2022). Gender-Related Biomechanical Properties of Masseter Muscle among Patients with Self-Assessment of Bruxism: A Comparative Study. J. Clin. Med., 11.10.3390/jcm11030845 ↗
8. Association between Myotonometric Measurement of Masseter Muscle Stiffness and Maximum Bite Force in Healthy Elders10.1111/joor.12968 ↗
9. Lee, K., and Chon, S. (2021). Assessments of Muscle Thickness and Tonicity of the Masseter and Sternocleidomastoid Muscles and Maximum Mouth Opening in Patients with Temporomandibular Disorder. Healthcare, 9.10.3390/healthcare9121640 ↗
10. Quantification of the Masseter Muscle Hardness of Stroke Patients Using the MyotonPRO Apparatus: Intra- and Inter-Rater Reliability and Its Correlation with Masticatory Performance
11. Interrater and Intrarater Reliability of a Handheld Myotonometer in Measuring Mechanical Properties of the Neck and Orofacial Muscles10.1016/j.jmpt.2020.08.002 ↗
12. Ultrasound Elastography in Temporomandibular Disorders: A Narrative Review
13. Comparison of Craniovertebral Angle and Muscle Properties after Smartphone Use in Healthy Individuals with and without Forward Head Posture
14. COVID-19 Pandemic and the Psyche, Bruxism, Temporomandibular Disorders Triangle10.1080/08869634.2021.1989768 ↗
15. Psychological Impact of COVID-19 Pandemic on TMD Subjects
16. Ginszt, M., Szkutnik, J., Zieliński, G., Bakalczuk, M., Stodółkiewicz, M., Litko-Rola, M., Ginszt, A., Rahnama, M., and Majcher, P. (2022). Cervical Myofascial Pain Is Associated with an Imbalance of Masticatory Muscle Activity. Int. J. Environ. Res. Public. Health, 19.10.3390/ijerph19031577 ↗
17. Minimally Invasive Techniques in the Management of Muscular Temporomandibular Joint Disorders: A Five-Year Observational Study10.4317/jced.61618 ↗
18. Short-Duration Therapeutic Massage Reduces Postural Upper Trapezius Muscle Activity10.1097/WNR.0000000000000718 ↗
19. Lee, S.Y., Bae, S.I., Do, S.-H., Sohn, J.-T., and Park, J.-W. (2020). The Effect of Brief Pre-Anesthetic Exercise Therapy of Jaw and Neck Joints on Mouth Opening, Neck Extension, and Intubation Conditions during Induction of General Anesthesia: A Randomized Controlled Trial. BMC Anesth., 20.10.1186/s12871-020-0939-8 ↗
20. Effects of Chewing Exercises on the Occlusal Force and Masseter Muscle Thickness in Community-Dwelling Koreans Aged 65 Years and Older: A Randomised Assessor-Blind Trial10.1111/joor.13036 ↗
21. Effects of Gum Chewing Exercise on Maximum Bite Force According to Facial Morphology10.1002/cre2.102 ↗
22. Effect of Gum Chewing Training on Masseter Muscle Oxygen Dynamics10.1007/978-3-031-67458-7_53 ↗
23. Effects of Functional Training after Orthognathic Surgery on Masticatory Function in Patients with Mandibular Prognathism10.1016/j.jds.2020.01.006 ↗
24. Oral Function in Patients with Myasthenia Gravis10.7717/peerj.11680 ↗
25. Analysis of Mechanical Properties of Cervical Muscles in Patients with Cervicogenic Headache10.1589/jpts.29.332 ↗
26. Guduru, R.K.R., Domeika, A., and Domeikienė, A. (2022). Effect of Rounded and Hunched Shoulder Postures on Myotonometric Measurements of Upper Body Muscles in Sedentary Workers. Appl. Sci., 12.10.3390/app12073333 ↗
27. An Investigation of the Changes in Mechanical Properties of the Orofacial and Neck Muscles between Patients with Myogenous and Mixed Temporomandibular Disorders10.1080/08869634.2021.1934795 ↗
28. Lo, W.L.A., Zhao, J.L., Li, L., Mao, Y.R., and Huang, D.F. (2017). Relative and Absolute Interrater Reliabilities of a Hand-Held Myotonometer to Quantify Mechanical Muscle Properties in Patients with Acute Stroke in an Inpatient Ward. Biomed. Res. Int., 2017.10.1155/2017/4294028 ↗
29. Mechanical Properties of Muscles around the Schoulder in Breast Cancer Patients: Intra-Rater and Inter-Rater Reliability of the MyotonPRO
30. Kutlutürk Yıkılmaz, S., Çevik Saldıran, T., Öztürk, Ö., and Öktem, S. (2024). Intersession Intra-Rater and Inter-Rater Reliability of Myotonometer for Upper and Lower Extremity Muscles in Children with Spinal Muscular Atrophy. Diagnostics, 14.10.3390/diagnostics14202300 ↗
31. Exploring the Interplay between Body Mass Index and Passive Muscle Properties in Relation to Grip Strength and Jump Performance in Female University Students10.7717/peerj.18430 ↗
32. Therapeutic Effects of Massage and Electrotherapy on Muscle Tone, Stiffness and Muscle Contraction Following Gastrocnemius Muscle Fatigue10.1589/jpts.29.144 ↗
33. Viscoelastic Properties of Lower Extremity Muscles after Elite Track Cycling Sprint Events: A Case Report
34. Zhu, Y., Feng, Y., Huang, F., Li, Y., Wang, W., Wang, X., Cao, X., and Zhang, Z. (2022). Changes in Stiffness of the Specific Regions of Knee Extensor Mechanism after Static Stretching. Front. Bioeng. Biotechnol., 10.10.3389/fbioe.2022.958242 ↗
35. Konrad, A., Seiberl, W., Tilp, M., Holzer, D., and Paternoster, F.K. (2022). What to Stretch?—Isolated Proprioceptive Neuromuscular Facilitation Stretching of Either Quadriceps or Triceps Surae Followed by Post-Stretching Activities Alters Tissue Stiffness and Jump Performance. Sports Biomech., 1–18.10.1080/14763141.2022.2058991 ↗
36. Effects of Cervical Stretching and Cranio-Cervical Flexion Exercises on Cervical Muscle Characteristics and Posture of Patients with Cervicogenic Headache10.1589/jpts.29.1836 ↗
37. Effects of Lumbar Lordosis Assistive Support on Craniovertebral Angle and Mechanical Properties of the Upper Trapezius Muscle in Subjects with Forward Head Posture10.1589/jpts.30.457 ↗
38. Changes in Muscle Stiffness of the Trapezius Muscle After Application of Ischemic Compression into Myofascial Trigger Points in Professional Basketball Players10.2478/hukin-2018-0043 ↗
39. Kim, H.-E. (2021). Influential Factors of Masticatory Performance in Older Adults: A Cross-Sectional Study. Int. J. Env. Res. Public. Health, 18.10.20944/preprints202104.0009.v1 ↗
40. Julià-Sánchez, S., Álvarez-Herms, J., Cirer-Sastre, R., Corbi, F., and Burtscher, M. (2019). The Influence of Dental Occlusion on Dynamic Balance and Muscular Tone. Front. Physiol., 10.10.3389/fphys.2019.01626 ↗
41. Creation of Myofascial Pain Syndrome-Like Muscle by Artificial Electrical Stimulation and Stretching Treatment10.1016/j.jneumeth.2023.109862 ↗
42. Effect of Muscle Energy Technique on Calf Muscle Stiffness Increased after Eccentric Exercise in Athletes10.5604/01.3001.0012.7985 ↗
43. Biomechanical Properties of Masseter Muscle Assessed through Myotonometry in Patients with Temporomandibular Disorder Treated with Ultrasound Therapy: A Randomized Comparative Study10.23736/S2724-6329.23.04855-6 ↗
44. Kisilewicz, A., Madeleine, P., Ignasiak, Z., Ciszek, B., Kawczynski, A., and Larsen, R.G. (2020). Eccentric Exercise Reduces Upper Trapezius Muscle Stiffness Assessed by Shear Wave Elastography and Myotonometry. Front. Bioeng. Biotechnol., 8.10.3389/fbioe.2020.00928 ↗
45. Villanueva, A., Rabal-Pelay, J., Berzosa, C., Gutiérrez, H., Cimarras-Otal, C., Lacarcel-Tejero, B., and Bataller-Cervero, A.V. (2020). Effect of a Long Exercise Program in the Reduction of Musculoskeletal Discomfort in Office Workers. Int. J. Env. Res. Public. Health, 17.10.3390/ijerph17239042 ↗
46. Invited Review: Aging and Sarcopenia10.1152/japplphysiol.00347.2003 ↗
47. Time of Day and Muscle Strength: A Circadian Output?10.1152/physiol.00030.2020 ↗
48. Potential Role of Passively Increased Muscle Temperature on Contractile Function10.1007/s00421-022-04991-7 ↗
49. Factor and Rasch Analysis of the Fonseca Anamnestic Index for the Diagnosis of Myogenous Temporomandibular Disorder10.1016/j.bjpt.2017.03.007 ↗
50. Analysis of the Accuracy and Reliability of the Short-Form Fonseca Anamnestic Index in the Diagnosis of Myogenous Temporomandibular Disorder in Women10.1016/j.bjpt.2018.02.003 ↗
51. Assessing the Viscoelastic Properties of Upper Trapezius Muscle: Intra- and Inter-Tester Reliability and the Effect of Shoulder Elevation10.1016/j.jelekin.2017.09.007 ↗
52. Influence of Tongue Exercise and Orofacial Myofunctional Status on the Electromyographic Activity and Pain of Chronic Painful TMD10.1080/08869634.2019.1656918 ↗
53. lmerTest Package: Tests in Linear Mixed Effects Models10.18637/jss.v082.i13 ↗
54. Cohen, J. (2013). Statistical Power Analysis for the Behavioral Sciences, Routledge. [2nd ed.].10.4324/9780203771587 ↗
55. Baumgart, C., Freiwald, J., Kühnemann, M., Hotfiel, T., Hüttel, M., and Hoppe, M.W. (2019). Foam Rolling of the Calf and Anterior Thigh: Biomechanical Loads and Acute Effects on Vertical Jump Height and Muscle Stiffness. Sports, 7.10.3390/sports7010027 ↗
56. The Effect of Manual Therapy on Gastrocnemius Muscle Stiffness in Healthy Individuals10.1016/j.foot.2019.01.006 ↗
57. Do, Y., Lall, P.S., and Lee, H. (2021). Assessing the Effects of Aging on Muscle Stiffness Using Shear Wave Elastography and Myotonometer. Healthcare, 9.10.3390/healthcare9121733 ↗
58. Diagnostic Accuracy of Tensiomyography Parameters for Monitoring Peripheral Neuromuscular Fatigue10.1016/j.jelekin.2023.102775 ↗
59. Park, S.J., Kim, S.H., and Kim, S.H. (2020). Effects of Thoracic Mobilization and Extension Exercise on Thoracic Alignment and Shoulder Function in Patients with Subacromial Impingement Syndrome: A Randomized Controlled Pilot Study. Healthcare, 8.10.3390/healthcare8030316 ↗
60. Cervical and Scapula-Focused Resistance Exercise Program versus Trapezius Massage in Patients with Chronic Neck Pain: A Randomized Controlled Trial10.1097/MD.0000000000030887 ↗
61. Effects on Neuromuscular Function After Ischemic Compression in Latent Trigger Points in the Gastrocnemius Muscles: A Randomized Within-Participant Clinical Trial10.1016/j.jmpt.2020.07.015 ↗
62. Myoton, A.S. (2012). MyotonPRO User Manual, Myoton AS.
63. Comparison of the Immediate Effect of Petrissage Massage and Manual Lymph Drainage Following Exercise on Biomechanical and Viscoelastic Properties of the Rectus Femoris Muscle in Women10.1123/jsr.2020-0276 ↗
64. Rey, E., Lois-Abal, M., Padrón-Cabo, A., Lorenzo-Martínez, M., and Costa, P.B. (2024). Influence of Training Load on Muscle Contractile Properties in Semi-Professional Female Soccer Players Across a Competitive Microcycle: A Pilot Study. Sensors, 24.10.3390/s24216996 ↗
65. ACTN3 Genotype Influences Masseter Muscle Characteristics and Self-Reported Bruxism10.1111/odi.14075 ↗
66. Unique Morphogenetic Signatures Define Mammalian Neck Muscles and Associated Connective Tissues10.7554/eLife.40179 ↗
67. Zieliński, G., Byś, A., Szkutnik, J., Majcher, P., and Ginszt, M. (2021). Electromyographic Patterns of Masticatory Muscles in Relation to Active Myofascial Trigger Points of the Upper Trapezius and Temporomandibular Disorders. Diagnostics, 11.10.3390/diagnostics11040580 ↗