Isolation, Characterization, and Genome Insight of Pseudomonas jordanii: A Novel Endophyte Enhancing Durum Wheat (Triticum turgidum ssp. durum) Growth under Salinity Stress
Source abstract
Abstract
Pseudomonas jordanii strain G34 is a moderately halophilic endophytic bacterium isolated from the root tissue of durum wheat plants growing in the saline environment of the Jordan Valley’s Ghor Sweimeh region. Microscopic and biochemical analyses of P. jordanii strain G34 revealed that it is a Gram-negative, non-motile rod. It also exhibits capsule formation, catalase and oxidase positive reactions, indole positivity, citrate utilization, and non-glucose fermenting capability. Pseudomonas jordanii strain G34 showed growth-promoting effects on durum wheat seedlings grown under severe salinity stress conditions up to a 200 mM NaCl concentration. The draft genome of P. jordanii strain G34 comprises 5,142,528 base pairs (bp) and possesses a G + C content of 64.0%. It contains 57 RNA coding genes and is predicted to encode a total of 4675 protein-coding genes. Putative genes linked to various aspects of the bacterial endophyte lifestyle were identified including ion transport, motility, secretion, adhesion, delivery systems, and plant cell wall modification. Performing a comprehensive phylogenomic analysis identified P. jordanii as a new species, with its closest relative being P. argentinensis LMG 22563, sharing only around 40.2% digital DNA-DNA hybridization identity. Pseudomonas jordanii strain G34 holds great potential for future use as a biofertilizer in saline environments.
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Research context
OpenAlex topics: Plant-Microbe Interactions and Immunity · Microbial Community Ecology and Physiology · Genomics and Phylogenetic Studies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Allied Medical Sciences, Zarqa University College, Al-Balqa Applied University, Al-Salt 19117, Jordan
Department of Biological Sciences, School of Science, The University of Jordan, Amman 11942, Jordan
Hamdi Mango Center for Scientific Research (HMCSR), The University of Jordan, Amman 11942, Jordan
Funding
Deanship of Scientific Research—The University of Jordan
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