In Vitro Comparison of Three-Dimensional Cultured Periodontal Ligament Stem Cells Derived from Permanent and Supernumerary Teeth
Source abstract
Abstract
The characteristics of 3-dimensional (3D) cultured periodontal ligament stem cells derived from permanent teeth (pPDLSCs) and supernumerary teeth (sPDLSCs) were compared and evaluated in this study. pPDLSCs and sPDLSCs were cultured in an ultra-low attachment 6-well plate, and the changes in morphology and size were observed under an optical microscope for 14 days. Cell counting kit-8 was used to quantitatively evaluate cell viability, and a live/dead assay was performed for the qualitative assessment. The degrees of osteogenic, chondrogenic, and adipogenic differentiation of pPDLSCs and sPDLSCs were also assessed. The pPDLSCs and sPDLSCs were initially spherical, and sPDLSCs were smaller than pPDLSCs. The viability of pPDLSCs decreased by 47.9% (day 3) and 10.4% (day 7), whereas that of sPDLSCs decreased by 34.3% (day 3) and 2.5% (day 7) compared to the percentage on Day 1. No significant difference in osteogenic differentiation was found (p = 0.94); however, significant differences in adipocyte and cartilage differentiation were observed (p = 0.003 and p = 0.013, respectively). Within the limitations of this study, sPDLSCs and pPDLSCs exhibited multilineage differentiation capacities, including osteogenic, chondrogenic, and adipogenic differentiation, in 3D culture. Overall, pPDLSCs should be further investigated as a source of stem cells for tissue engineering and regenerative medicine.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Mesenchymal stem cell research · Periodontal Regeneration and Treatments · Tissue Engineering and Regenerative Medicine
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Dentistry, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea
Department of Pediatric Dentistry, Kyung Hee University Medical Center, Seoul 02447, Republic of Korea
Department of Pediatric Dentistry, School of Dentistry, Kyung Hee University, Seoul 02447, Republic of Korea
Department of Pediatric Dentistry, Kyung Hee University College of Dentistry, Kyung Hee University Hospital at Gangdong, Seoul 05278, Republic of Korea
Department of Conservative Dentistry, School of Dentistry, Kyung Hee University, Seoul 02447, Republic of Korea
Funding
Bio and Medical Technology Development Program of the National Research Foundation (NRF) · Korean government (MSIP and MOHW) NRF
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