Synthetic Biology and Control Theory: Designing Synthetic Biomolecular Controllers by Exploiting Dynamic Covalent Modification Cycle with Positive Autoregulation Properties
Source abstract
Abstract
The emerging field at the intersection of synthetic biology, network modelling, and control theory has grown in popularity in recent years. In this study, the aim is to design synthetic biomolecular controllers by exploiting the covalent modification cycle (CMC) enhanced with direct and indirect positive autoregulation (DPAR and IPAR). Two novel biomolecular controllers proposed, the Brink controller (BC) with DPAR (namely BC-DPAR) and the BC with IPAR (namely BC-IPAR), allow to (a) use fewer chemical reactions than purely designs based on dual chemical reaction networks (DCRNs), and (b) improve the stability of ultrasensitive response when designing biomolecular controllers. Following the conversion route from chemical reactions to DNA strand displacements, the integration of the two novel controllers and an enzymatic proteolysis model proposed aims to analyse the regulatory properties by exploring the tracking response of proteolysis products.
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Research context
OpenAlex topics: Gene Regulatory Network Analysis · Molecular Communication and Nanonetworks · Receptor Mechanisms and Signaling
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Key Laboratory of Advanced Design and Intelligent Computing, Ministry of Education, School of Software Engineering, Dalian University, Dalian 116622, China
State Key Laboratory of Synthetical Automation for Process Industries, Northeastern University, Shenyang 110004, China
College of Environmental and Chemical Engineering, Dalian University, Dalian 116622, China
Dalian Chivy Biotechnology Co., Ltd., Dalian 116023, China
Funding
111 Project · National Natural Science Foundation of China · Liaoning Revitalization Talents Program · Natural Science Foundation of Liaoning Province · Scientific Research Fund of Liaoning Provincial Education Department · State Key Laboratory of Synthetical Automation for Process Industries · State Key Laboratory of Light Alloy Casting Technology for High-end Equipment · Postgraduate Education Reform Project of Liaoning province · Dalian Outstanding Young Science and Technology Talent Support Program
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