Immunohistochemical Expression Patterns of Tight Junction Proteins, Pro-Apoptotic and Anti-Apoptotic Factors on Progression of Intestinal Mucositis of Onco-Hematological Patients under Epirubicin-Based Chemotherapy
Source abstract
Abstract
Chemotherapy and radiation are often accompanied by complications such as intestinal mucositis. The aim of this study was to assess by immunohistochemical assay the consequences of epirubicin-based therapy applied to onco-hematological patients, on the mucosal cells that undergo apoptosis and on the tight junction proteins, immediately before and after a short time of chemotherapy administration. We assessed the protein expression and distribution of the pro-apoptotic Bax, anti-apoptotic Bcl-2 and effector Caspase-3 as key proteins in apoptosis pathways and the changes in immunopositivity of Claudin-1 and ZO-1 tight junction proteins. Results show that the Bcl-2 family is involved in intestinal damage via Caspase-3 dependent apoptosis of epithelial cells. Additionally, the intestinal mucositis activates other injurious pathways through a dramatic drop in Claudin-1 and ZO-1 expressions, contributing for a while to a structural and functional integrity disruption of the intestinal epithelium.
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Research context
OpenAlex topics: Barrier Structure and Function Studies · Cancer-related molecular mechanisms research · Neurological Disease Mechanisms and Treatments
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Hematology, Faculty of Medicine, Vasile Goldis Western University of Arad, 310025 Arad, Romania
Department of Gastroenterology, Faculty of Medicine, Vasile Goldis Western University of Arad, 310025 Arad, Romania
“Aurel Ardelean” Institute of Life Sciences, Vasile Goldis Western University of Arad, 310025 Arad, Romania
Department of Histology, Faculty of Medicine, Vasile Goldis Western University of Arad, 310025 Arad, Romania
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