A Three-Dimensional Tracking Method with the Self-Calibration Functions of Coaxiality and Magnification for Single Fluorescent Nanoparticles
Source abstract
Abstract
A self-calibrating variable magnification three-dimensional (3D) tracking system for single fluorescent nanoparticles is proposed. The system was based on astigmatic micro-imaging and has a simple configuration incorporating a dual-spot position detection unit ray transfer matrix. By analyzing this matrix and utilizing the beam vector measurement of a dual-spot position detection unit, it was demonstrated that the proposed tracking system had the self-calibration functions of coaxiality and magnification; thus, it could accurately track the adopted fluorescent nanoparticles through zoom microscopical measurements in 3D. The available measurements of the proposed system and accuracy were experimentally validated.
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Research context
OpenAlex topics: Advanced Fluorescence Microscopy Techniques · Optical Coherence Tomography Applications · Photoacoustic and Ultrasonic Imaging
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
School of Electrical and Electronic Engineering, Shandong University of Technology, Zibo 255000, China
Funding
Shandong Provincial Natural Science Foundation, China · China Postdoctoral Science Foundation · National Natural Science Foundation of China
References (28)
Show source-provided references
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