Distinctive Culture Expressions of Enterobacteria Interfering with Isolation of Salmonella spp. during the Application of the Recommended ISO 6579-1:2017
Source abstract
Abstract
The objective of the present report is the dissemination of information acquired during the application of ISO 6579-1:2017 for the isolation of Salmonella spp. from swine samples. ISO 6579-1:2017 is the protocol officially recommended by the EU for the isolation of Salmonella spp., aiming for the harmonization of effective control of Salmonella infection in food-producing animals. Successful control of animal salmonellosis is highly dependent on the sensitivity and reliability of the biochemical methods used to detect the presence of the pathogen in various stages of food production. Application of ISO 6579-1:2017 resulted in the isolation of twelve Salmonella spp. and eighteen other members of the family of Enterobacteriaceae biochemically and other bacteria antigenically resembling salmonellae. An evaluation of the culture media was conducted, including xylose-lysine-desoxycholate agar (XLD), Salmonella–Shigella Agar (SS), Brilliant Green Agar (BGA), Salmonella Chromogenic Agar (SCA), Triple Sugar Iron (TSI), and modified semi-solid Rappaport–Vassiliadis (MSRV) agar. The evaluation showed that these culture-selective media differed significantly in their performance for the isolation of Salmonella from swine samples. It was concluded that the presence of atypical Salmonella strains negatively affects the prevalence of Salmonella, thus affecting the identification of carrier pigs and eventually affecting the efficiency of control programs. Thus, doubtful results require additional biochemical testing to confirm the accuracy of such universally recommended isolation methods.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Salmonella and Campylobacter epidemiology · Listeria monocytogenes in Food Safety · Viral gastroenteritis research and epidemiology
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Laboratory of Hygiene of Foods of Animal Origin, Faculty of Veterinary Medicine, University of Thessaly, 224 Trikalon Street, 43100 Karditsa, Greece
Department of Nursing, University of Peloponnese, 23100 Tripoli, Greece
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