Characterization of Escherichia coli Cefotaxime-Resistance in Al-Ahsa, KSA: Predominance of CTX-15 and First Report of blaCMY-42 Gene
Source abstract
Abstract
We determined an antibiotic resistance mechanism in the eastern region, KSA, and the genetic factor clonal relatedness within Gram-negative bacteria. During our retrospective study, a total number of 29 E. coli ESBL producer strains were isolated for patients visiting King Fahad Hospital, Al-Ahsa, KSA. The bla genes were detected via PCR and identified via sequencing. Associated plasmid-mediated quinolone resistance genes, as well as int1 and int2 genes, were also studied. Phylogenetic groups, the ST131 clone, virulence factors, and PFGE were also checked. The blaCTX-M-9 (3.7%), blaCTX-M-27 (22.2%), and blaCTX-M-15 (77.8%) genes were identified; however, the blaCMY-42 (7.4%) gene was recorded for the first time in KSA. The qnrS1 gene was found in 44.4% of strains, and among them, 50% concomitantly harbored the aac(6′)Ib-cr. The int1 gene was detected in 25.9% strains; nonetheless, the int2 gene was found in 7.4% of isolates. The strains belonged mainly to the B2 and D phylogroups. PFGE showed unrelated patterns. Some isolates belonged to the pandemic clone ST131. We describe a large dissemination of antibiotic resistance to third-generation cephalosporins in the eastern region, KSA, with the occurrence of the blaCMY-42 gene. The clone ST131 seems to be the principal contributor for blaCTX-M-15 gene spread.
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Research context
OpenAlex topics: Antibiotic Resistance in Bacteria · Antibiotics Pharmacokinetics and Efficacy · Escherichia coli research studies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Applied College in Abqaiq, King Faisal University, Al-Ahsa 31982, Saudi Arabia
Laboratory of “Resistance to Antimicrobiens” LR99ES09, College of Medicine, Tunis El Manar University, Tunis 1007, Tunisia
Laboratory of Bacteriological Research, Tunisian Institute of Veterinary Research, Tunis El Manar University, Tunis 1006, Tunisia
Chemistry Department, College of Science, King Faisal University, Al-Ahsa 31982, Saudi Arabia
Laboratory of Microbiology, Charles-Nicolle Hospital, Tunis 1006, Tunisia
Funding
King Faisal University · Deanship of Scientific Research of King Faisal University
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