DataVXApplied SciencesPaper
journal article·2022-05-25Open access

A Method to Produce vsiRNAs in Plants with Cross-Kingdom Gene Silencing Capacity

Hernán de Jesús Villanueva-Alonzo, Ana Paulina Haro-Álvarez, Arturo A. Alvarado-Segura, Raúl Enrique Valle-Gough, Juan Gualberto Collí-Mull, Alberto Cal-Torres, Víctor Ermilo Arana-Argáez, Julio César Torres-Romero, Oscar Alberto Moreno-Valenzuela, Geovanny Nic-Can, Benjamín Abraham Ayil-Gutiérrez, Karla Y. Acosta-Viana

Applied Sciences· Volume 12· Issue 11
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Source abstract

Abstract

Plants have evolved defense mechanisms to suppress viral transcription and replication by transcriptional and post-transcriptional gene silencing mediated by virus-derived small interfering RNAs (vsiRNAs). Based on this response, virus-induced gene silencing (VIGS)-based technology has been developed to silence target genes on either host plants or insect pests. This mechanism could also be used for the silencing of genes of interest in the medical field. We used the VIGS vector pEuMV-YP:Krt18, which was obtained by inserting the Mus musculus (M. musculus) Krt18 sequence into pEuMV-YP:ΔAV1. The objective was to evaluate the capacity of pEuMV-YP:Krt18 to induce Nicotiana benthamiana (N. benthamiana) production of vsiRNAs of a specific sequence that belongs to neither the plant genome nor the wild virus genome, which were used to induce cross-kingdom gene silencing between plants and mammals. The percentage of vsiRNA for each viral gene was calculated from an sRNA library of N. benthamiana plants infected by pEuMV-YP: Krt18. When the vsiRNAs were characterized, it was found that they corresponded to all the genes of the pEuMV-YP:Krt18 vector. These vsiRNAs induced the silencing of the Krt18 gene in M. musculus macrophages, supporting the ability to use VIGS vectors in plants as biofactories for the production of sRNAs that induce gene silencing in mammals.
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Research context

OpenAlex topics: Plant Virus Research Studies · CRISPR and Genetic Engineering · Plant and Fungal Interactions Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Centro de Investigaciones Regionales “Dr. Hideyo Noguchi”, CONACyT-Universidad Autónoma de Yucatán, Av. Itzáes, núm. 490 x calle 59, col. Centro, Mérida 97000, Yucatán, Mexico

Instituto de Investigaciones en Ciencias Veterinarias, Universidad Autónoma de Baja California, Carretera, Mexicali-San Felipe Km 3.5, Laguna Campestre, Mexicali 21705, Baja California, Mexico

Instituto Tecnológico Superior del Sur del Estado de Yucatán, Tecnológico Nacional de México, Carretera Muna-Felipe Carrillo Puerto Tramo Oxkutzcab-Akil km 41+400, Oxkutzcab 97880, Yucatán, Mexico

Instituto de Ciencias Agrícolas, Universidad Autónoma de Baja California, sin núm., Ejido Nuevo León, Mexicali 21705, Baja California, Mexico

Instituto Tecnológico Superior de Irapuato, Tecnológico Nacional de México, Carretera Irapuato—Silao km 12.5 Colonia El Copal, Irapuato 36580, Guanajuato, Mexico

Centro de Investigaciones Regionales “Dr. Hideyo Noguchi”, Universidad Autónoma de Yucatán, Av. Itzáes, núm. 490 x calle 59, col. Centro, Mérida 97000, Yucatán, Mexico

Facultad de Química, Universidad Autónoma de Yucatán, Calle 43 No. 613 x Calle 90, Col. Inalámbrica, Mérida 97069, Yucatán, Mexico

Unidad de Bioquímica, Centro de Investigación Científica de Yucatán, A.C., Calle 43 No. 130. Col. Chuburná de Hidalgo, Mérida 97000, Yucatán, Mexico

Facultad de Ingeniería Química, CONACyT-Universidad Autónoma de Yucatán, Periférico Norte, Km. 33.5. Tablaje Catastral 13615. Col. Chuburná de Hidalgo Inn., Mérida 97203, Yucatán, Mexico

Centro de Biotecnología Genómica, CONACyT-Instituto Politécnico Nacional, Blvd. del Maestro, sin núm. esquina, Reynosa 88710, Tamaulipas, Mexico

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