Candida albicans Antimicrobial and Antibiofilm Activity of Novel Endodontic Solvents
Source abstract
Abstract
Background: Candida albicans is the most prevalent fungi isolated in endodontic infections. In this study, the ability of C. albicans biofilm to tolerate the novel solvent mixtures methyl ethyl ketone (MEK)/tetrachloroethylene (TCE) and MEK/orange oil (OOil) sequentially to the standard irrigation of sodium hypochlorite (NaOCl) and ethylenediaminetetraacetic (EDTA) was evaluated. Methods: Biofilm cell cultures of C. albicans SC 5314 were treated sequentially with NaOCl and EDTA and exposed to MEK/TCE or MEK/OOil. The effect of the antimicrobial treatment was evaluated using the disk diffusion method for planktonic culture, and the enumeration of colony-forming units (CFUs) and scanning electron microscope (SEM) for biofilm cell culture. Results: C. albicans mature biofilm (24 h) was significantly reduced in biomass and cell viability after solvent mixtures’ exposure, compared with the previous NaOCl and EDTA treatments. MEK/OOil combination caused a total reduction of biofilm, while with MEK/TCE, there was a 3-log (CFU/cm2) reduction compared with the sequence NaOCl and EDTA, and a 4-log (CFU/cm2) reduction compared with the control. Conclusions: The additional exposure of a preformed 24 h C. albicans biofilm to novel solvent mixtures MEK/TCE and MEK/OOil caused a positive antibiofilm impact, overcoming the performance of the conventional endodontic irrigating protocol.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Endodontics and Root Canal Treatments · Oral microbiology and periodontitis research · Dental materials and restorations
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
CINTESIS, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal
Centre of Biological Engineering, LIBRO–Laboratório de Microbiologia Aplicada à Saúde, Campus de Gualtar, University of Minho, 4710-057 Braga, Portugal
CINTESIS, Faculty of Dental Medicine, University of Porto, 4200-393 Porto, Portugal
Funding
Fundação para a Ciência e a Tecnologia
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