Genetic Deletion of Interleukin-15 Is Not Associated with Major Structural Changes Following Experimental Post-Traumatic Knee Osteoarthritis in Rats
Source abstract
Abstract
Post-traumatic osteoarthritis (PTOA) is a degenerative joint disease, leading to articular cartilage breakdown, osteophyte formation, and synovitis, caused by an initial joint trauma. Pro-inflammatory cytokines increase catabolic activity and may perpetuate inflammation following joint trauma. Interleukin-15 (IL-15), a pro-inflammatory cytokine, is increased in OA patients, although its roles in PTOA pathophysiology are not well characterized. Here, we utilized Il15 deficient rats to examine the role of IL-15 in PTOA pathogenesis in an injury-induced model. OA was surgically induced in Il15 deficient Holtzman Sprague-Dawley rats and control wild-type rats to compare PTOA progression. Semi-quantitative scoring of the articular cartilage, subchondral bone, osteophyte size, and synovium was performed by two blinded observers. There was no significant difference between Il15 deficient rats and wild-type rats following PTOA-induction across articular cartilage damage, subchondral bone damage, and osteophyte scoring. Similarly, synovitis scoring across six parameters found no significant difference between genetic variants. Overall, IL-15 does not appear to play a key role in the development of structural changes in this surgically-induced rat model of PTOA.
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Research context
OpenAlex topics: Osteoarthritis Treatment and Mechanisms · Knee injuries and reconstruction techniques · Lower Extremity Biomechanics and Pathologies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Physiology and Pharmacology, Schulich School of Medicine & Dentistry, Western University, 1151 Richmond St., London, ON N6A 3K7, Canada
Bone and Joint Institute, Western University, 1151 Richmond St., London, ON N6A 3K7, Canada
Department of Microbiology and Immunology, Schulich School of Medicine & Dentistry, Western University, 1151 Richmond St., London, ON N6A 3K7, Canada
Department of Anatomy and Cell Biology, Schulich School of Medicine & Dentistry, Western University, 1151 Richmond St., London, ON N6A 3K7, Canada
Department of Medicine, Schulich School of Medicine & Dentistry, Western University, 1151 Richmond St., London, ON N6A 3K7, Canada
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