journal article·2021-07-24Open access

Microscope Enclosure for Temperature Regulation and Light Isolation

Daniel S. Johnson, Taylor Deneau, Ricardo Toledo-Crow, Sanford M. Simon

Applied Sciences· Volume 11· Issue 15

Abstract

Light isolation and temperature regulation are often required for microscopic imaging. Commercial enclosures are available to satisfy these requirements, but they are often not flexible to the variety of custom systems found in research laboratories. We present the design for an affordable enclosure which utilizes aluminum t-slot profiles to support opaque expanded PVC panels. Temperature is regulated by exchanging the enclosure air with an external heater. In addition, we demonstrate baffles integrated into the enclosure improve temperature uniformity. Example designs for both upright and inverted microscopes are given, providing a starting point for creating a system-specific custom enclosure.
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Research context

OpenAlex topics: Advanced Fluorescence Microscopy Techniques · Cell Image Analysis Techniques · Digital Holography and Microscopy

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Physics and Astronomy, Hofstra University, Hempstead, New York, NY 11549, USA

Next Generation Environmental Sensor Laboratory, City University of New York, New York, NY 10031, USA

Laboratory of Cellular Biophysics, The Rockefeller University, New York, NY 10065, USA

Funding

National Institutes of Health

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