journal article·2021-06-22Open access

The Factor VII Variant p.A354V-p.P464Hfs: Clinical versus Intracellular and Biochemical Phenotypes Induced by Chemical Chaperones

Elisabeth Andersen, Maria Eugenia Chollet, Francesco Bernardi, Alessio Branchini, Marcello Baroni, Guglielmo Mariani, Alberto Dolce, Angelika Batorova, Ellen Skarpen, Christiane Filion Myklebust, Grethe Skretting, Per Morten Sandset

Applied Sciences· Volume 11· Issue 13

Abstract

(1) Background: Congenital factor (F) VII deficiency is caused by mutations in the F7 gene. Patients with modest differences in FVII levels may display large differences in clinical severity. The variant p.A354V-p.P464Hfs is associated with reduced FVII antigen and activity. The aim of the study was to investigate the clinical manifestation of this variant and the underlying molecular mechanisms. (2) Methods: Analyses were conducted in 37 homozygous patients. The recombinant variant was produced in mammalian cells. (3) Results: We report a large variation in clinical phenotypes, which points out genetic and acquired components beyond F7 mutations as a source of variability. In contrast, patients displayed similarly reduced FVII plasma levels with antigen higher than its activity. Comparative analysis of the recombinant variant and of plasma samples from a subset of patients indicated the presence of an elongated variant with indistinguishable migration. Treatment of cells with the chemical chaperone 4-phenylbutyrate (4-PBA) improved the intracellular trafficking of the variant and increased its secretion to the conditioned medium up to 2-fold. However, the effect of 4-PBA on biological activity was marginal. (4) Conclusions: Chemical chaperones can be used as biochemical tools to study the intracellular fate of a trafficking-defective FVII variant.
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Research context

OpenAlex topics: Hemophilia Treatment and Research · Blood properties and coagulation · Erythrocyte Function and Pathophysiology

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Haematology, Oslo University Hospital, 0450 Oslo, Norway

Research Institute of Internal Medicine, Oslo University Hospital, 0450 Oslo, Norway

Department of Life Sciences and Biotechnology and LTTA Centre, University of Ferrara, 44121 Ferrara, Italy

Faculty of Science and Technology, Westminster University, London W1B 2HW, UK

Seven Treatment Evaluation Registry [STER] Study Group, 0450 Oslo, Norway

National Institute of Statistics, 90100 Palermo, Italy

The National Haemophilia Centre, Institute of Haematology and Blood Transfusion, School of Medicine of Comenius University, University Hospital, 813 72 Bratislava, Slovakia

Core Facility for Advanced Light Microscopy, Institute for Cancer Research, Oslo University Hospital, 0379 Oslo, Norway

Institute of Clinical Medicine, University of Oslo, 0318 Oslo, Norway

Funding

Helse Sør-Øst RHF

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