Monitoring the Antibacterial Effect of Rosin Acids in an Austrian Beet Sugar Plant by Amplicon-Based Sequencing and Flow Cytometry
Source abstract
Abstract
For decades, microorganisms in beet sugar production have been studied using culture-based methods. However, these methods are not sufficient to describe such a complex bacterial community. In this study, therefore, an amplicon-based sequencing technique (Illumina MiSeq platform) was applied to characterize the bacterial community and its dynamics in the extraction area and juice purification station of an Austrian beet sugar plant. Depending on the process conditions thermophilic bacteria, such as Geobacillus spp., Caenibacillus spp., and Thermus spp., and mesophilic bacteria, such as Leuconostoc spp. and Bacillus spp., were found. Besides these microbiological characteristics, the antimicrobial effect of a rosin acid-based product (Defostab 220) on the bacterial communities was investigated in industrial and laboratory trials. The antimicrobial effect of a given concentration of rosin acid varies from bacteriostatic to bactericidal effects on different occurring groups of bacteria.
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Research context
OpenAlex topics: Microbial Metabolism and Applications · Microbial Natural Products and Biosynthesis · Microbial Metabolic Engineering and Bioproduction
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
AGRANA Research & Innovation Center GmbH, Department of Sugar Technology, Josef-Reither-Straße 21-23, A-3430 Tulln, Austria
Department of Food Science and Technology, Institute of Food Science, BOKU–University of Natural Resources and Life Sciences Vienna, Muthgasse 18, A-1190 Vienna, Austria
Funding
Österreichische Forschungsförderungsgesellschaft
References (60)
Show source-provided references
1. Van der Poel, P.W. (2000). Zuckertechnologie. Rüben- und Rohrzuckergewinnung, Dr. Albert Bartens KG.
2. Van der Poel, P.W., Schiweck, H., and Schwartz, T. (2000). Mikrobiologie. Zuckertechnologie. Rüben- und Rohrzuckergewinnung, Dr. Albert Bartens KG.
3. Krüger, W. (1957, January 26). Über Infektion und Zuckerverlust im Diffusionsturm. Proceedings of the Commission Internationale Technique de Sucrerie, Frankfurt, Germany.
4. Evaluation expérimentale des pertes de sucre d’origine microbienne en diffusion
5. Eine einfache Methode zur quantitativen Bestimmung mikrobieller Stoffumsetzungen mittels Objektträgerkulturen
6. Zur Taxonomie der hochthermophilen, in Zuckerfabriksäften vorkommenden aeroben Sporenbildner
7. Saccharolytic bacteria in beet juices
8. Microbiological counts during beet sugar extraction10.4315/0362-028X-72.6.1332 ↗
9. Recent advances in molecular techniques to study microbial communities in food-associated matrices and processes10.1016/j.fm.2008.04.009 ↗
10. Franco-Duarte, R., Černáková, L., Kadam, S., Kaushik, K.S., Salehi, B., Bevilacqua, A., Corbo, M.R., Antolak, H., Dybka-Stępień, K., and Leszczewicz, M. (2019). Advances in Chemical and Biological Methods to Identify Microorganisms-From Past to Present. Microorganisms, 7.10.3390/microorganisms7050130 ↗
11. Protective effect of hop β-acids on microbial degradation of thick juice during storage
12. Identification of main bacteria and fungi found during beet sugar extraction in Spanish factories
13. Bacterial growth dynamics and corresponding metabolite levels in the extraction area of an Austrian sugar beet factory using antimicrobial treatment10.1002/jsfa.10303 ↗
14. Application of flow cytometry to industrial microbial bioprocesses10.1016/j.bej.2009.07.013 ↗
15. Application of flow cytometry to wine microorganisms10.1016/j.fm.2016.10.023 ↗
16. Determination of the effect of plant essential oils obtained by supercritical fluid extraction on the growth and viability of Listeria monocytogenes in broth and food systems using flow cytometry10.1016/j.lwt.2008.06.007 ↗
17. Application of hop-β-acids and rosin acids in the sugar industry
18. 10 years’ experience with natural antibacterials within Agrana
19. Andrews, S. (2019, June 28). Fast QC. Available online: http://www.bioinformatics.babraham.ac.uk/projects/fastqc/.
20. Cutadapt removes adapter sequences from high-throughput sequencing reads10.14806/ej.17.1.200 ↗
21. Search and clustering orders of magnitude faster than BLAST10.1093/bioinformatics/btq461 ↗
22. Edgar, R.C. (2016). UNOISE2: Improved error-correction for Illumina 16S and ITS amplicon sequencing. BioRxiv, 081257.10.1101/081257 ↗
23. Edgar, R.C. (2017). UNBIAS: An attempt to correct abundance bias in 16S sequencing, with limited success. BioRxiv, 124149.10.1101/124149 ↗
24. Edgar, R.C. (2018). UNCROSS2: Identification of cross-talk in 16S rRNA OTU tables. BioRxiv, 400762.10.1101/400762 ↗
25. Edgar, R.C. (2016). SINTAX: A simple non-Bayesian taxonomy classifier for 16S and ITS sequences. BioRxiv, 074161.10.1101/074161 ↗
26. Ribosomal Database Project: Data and tools for high throughput rRNA analysis10.1093/nar/gkt1244 ↗
27. Ondov, B.D., Bergman, N.H., and Phillippy, A.M. (2011). Interactive metagenomic visualization in a Web browser. BMC Bioinf., 12.10.1186/1471-2105-12-385 ↗
28. Comparison of the levels of bacterial diversity in freshwater, intertidal wetland, and marine sediments by using millions of illumina tags10.1128/AEM.01821-12 ↗
29. Hubbell’s fundamental biodiversity parameter and the Simpson diversity index10.1111/j.1461-0248.2005.00729.x ↗
30. Characterization of microbial community structure and metabolic potential using Illumina MiSeq platform during the black garlic processing10.1016/j.foodres.2017.12.081 ↗
31. Flow cytometric viability assessment of lactic acid bacteria starter cultures produced by fluidized bed drying10.1007/s00253-014-5592-z ↗
32. Dr. Müller Gerätebau GmbH (2011). Gebrauchsanweisung—Super GL Compact, Dr. Müller Gerätebau GmbH. Version 2.0.
33. Tumebacillus permanentifrigoris gen. nov., sp. nov., an aerobic, spore-forming bacterium isolated from Canadian high Arctic permafrost10.1099/ijs.0.65101-0 ↗
34. Holzapfel, W.H., and Wood, B.J.B. (2014). The genera Bacillus, Geobacillus and Halobacillus. Lactic Acid Bacteria: Biodiversity and Taxonomy, Wiley.10.1002/9781118655252 ↗
35. Complete genome sequence of thermophilic Bacillus smithii type strain DSM 4216(T)10.1186/s40793-016-0172-8 ↗
36. Reclassification of Saccharococcus caldoxylosilyticus as Geobacillus caldoxylosilyticus (Ahmad et al. 2000) comb. nov10.1099/00207713-51-6-2063 ↗
37. Geobacillus toebil subsp. decanicus subsp. nov., a hydrocarbon-degrading, heavy metal resistant bacterium from hot compost10.2323/jgam.52.223 ↗
38. Geobacillus toebii sp. nov., a novel thermophilic bacterium isolated from hay compost10.1099/00207713-52-6-2251 ↗
39. Anoxybacillus thermarum sp. nov., a novel thermophilic bacterium isolated from thermal mud in Euganean hot springs, Abano Terme, Italy10.1007/s00792-009-0274-y ↗
40. Caenibacillus caldisaponilyticus gen. nov., sp. nov., a thermophilic, spore-forming and phospholipid-degrading bacterium isolated from acidulocompost10.1099/ijsem.0.001108 ↗
41. Holzapfel, W.H., and Wood, B.J.B. (2014). The genus Leuconostoc. Lactic Acid Bacteria: Biodiversity and Taxonomy, Wiley.10.1002/9781118655252 ↗
42. Holzapfel, W.H., and Wood, B.J.B. (2014). The genus Fructobacillus. Lactic Acid Bacteria: Biodiversity and Taxonomy, Wiley.10.1002/9781118655252 ↗
43. Holzapfel, W.H., and Wood, B.J.B. (2014). The genus Lactobacillus. Lactic Acid Bacteria: Biodiversity and Taxonomy, Wiley.10.1002/9781118655252 ↗
44. Differentiation of Lactobacillus plantarum, L. pentosus, and L. paraplantarum by recA gene sequence analysis and multiplex PCR assay with recA gene-derived primers10.1128/AEM.67.8.3450-3454.2001 ↗
45. Rothia aeria sp. nov., Rhodococcus baikonurensis sp. nov. and Arthrobacter russicus sp. nov., isolated from air in the Russian space laboratory Mir10.1099/ijs.0.02828-0 ↗
46. Four novel Arthrobacter species isolated from filtration substrate10.1099/ijs.0.65301-0 ↗
47. Classification of Ralstonia pickettii-like isolates from the environment and clinical samples as Ralstonia insidiosa sp. nov10.1099/ijs.0.02555-0 ↗
48. Burkholderia fungorum sp. nov. and Burkholderia caledonica sp. nov., two new species isolated from the environment, animals and human clinical samples10.1099/00207713-51-3-1099 ↗
49. Diversity and significance of Burkholderia species occupying diverse ecological niches10.1046/j.1462-2920.2003.00471.x ↗
50. Transferable denitrification capability of Thermus thermophilus10.1128/AEM.02594-13 ↗
51. Nitrit formation in the thin juice by Thermus species
52. Isolation of Thermus Strains from Hot Composts (60 to 80 °C)10.1128/aem.62.5.1723-1727.1996 ↗
53. Illumina-based analysis of endophytic bacterial diversity and space-time dynamics in sugar beet on the north slope of Tianshan mountain
54. Nemec, A. (2018). Leistungsspezifische Charakterisierung von Schwachsaurem Kationentauscherharz in Verschiedenen Medien. [Bachelor’s Thesis, Universität für Bodenkultur].
55. Integrative transcriptomics reveals genotypic impact on sugar beet storability10.1007/s11103-020-01041-8 ↗
56. Changes in the processing quality of sugar beet (Beta vulgaris L.) during long-term storage under controlled conditions10.1111/j.1365-2621.2007.01641.x ↗
57. New approaches for the determination of dextran in the sugar production process
58. Pollach, G., and Hein, W. (2006). Method for Producing Sugar by Aid of Resins. (1,282,731B1), Austria Patent.
59. The role of plant-based antimicrobials in food and feed production with special regard to silage fermentation
60. Bubnik, Z., Kadlec, P., Urban, D., and Bruhns, M. (1995). Sugar Technologists Manual, Dr. Albert Bartens KG.