Application of X-ray Spectroscopic Techniques to Determine the Inorganic Composition and Sulfur Chemical Speciation of the Amazonian Plant Bixa orellana
Source abstract
Abstract
Bixa orellana is a plant that has a variety of uses, such as applications in the food and cosmetic industries, as well as culinary uses, and body painting for Indigenous people. Despite its versatility, few studies have explored its inorganic composition, and its sulfur chemical speciation has only been assessed from the point of view of sulfurous amino acids. Here, we report on the inorganic composition of Bixa orellana fruits, pericarps, and seeds obtained using Wavelength Dispersive X-ray Fluorescence (WD-XRF) and sulfur chemical speciation using X-ray Absorption Near-Edge Structure (XANES). Our results show that the seed is a source of potassium, calcium, magnesium, and phosphorous. But also, the pericarp, which is considered waste, contains a high amount of nutrients. From the XANES measurements, the distribution of the oxidation state of the sulfur atom was obtained, and it was shown that although several oxidation states of sulfur are present, oxidized sulfur (sulfate) is the dominant form of sulfur in all samples.
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Research context
OpenAlex topics: Heavy Metals in Plants · Plant biochemistry and biosynthesis · X-ray Spectroscopy and Fluorescence Analysis
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Faculty of Engineering, University of the Republic, Montevideo 11300, Uruguay
Department of Physical-Chemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro CEP 21941-909, RJ, Brazil
Federal Institute of Rio de Janeiro, Campus Duque de Caxias, Duque de Caxias CEP 25050-100, RJ, Brazil
Institute of Physics, Federal University of Rio de Janeiro, Rio de Janeiro CEP 21941-909, RJ, Brazil
Bioprospection and Biotechnology Laboratory, Technology and Innovation Coordination, National Institute of Amazonian Research, Manaus CEP 69067-375, AM, Brazil
Department of Botany, University of Brasilia, Brasilia CEP 70910-900, DF, Brazil
Funding
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) · Udelar · CNPq · FAPERJ · FAPEAM · LNLS
References (60)
Show source-provided references
1. Antar, G.M., and do Rio de Janeiro Jardim, B. (2023, May 17). Flora e Funga Do Brasil, Available online: https://floradobrasil.jbrj.gov.br/FB5745.
2. Stringheta, P.C., and Silva, P.I. (2008). Pigmentos de Urucum: Extração, Reações Químicas, Usos e Aplicações, Gráfica e Editora Suprema. [1st ed.].
3. The Domestication of Annatto (Bixa orellana) from Bixa Urucurana in Amazonia10.1007/s12231-015-9304-0 ↗
4. Other Herbs and Spices: Achiote to Szechuan Pepper10.1533/9780857095688.534 ↗
5. Optical Characterization in Annatto and Commercial Colorific10.1007/s10895-010-0730-1 ↗
6. The Comet Assay with 8 Mouse Organs: Results with 39 Currently Used Food Additives10.1016/S1383-5718(02)00128-6 ↗
7. Updates on Chemistry and Use of Annatto (Bixa orellana L.)
8. Chemical composition, amino acid content and nutritive value of the protein of the annatto seed (Bixa orellana L.)
9. Achiote (Bixa orellana L.): A Natural Source of Pigment and Vitamin E10.1007/s13197-017-2579-7 ↗
10. Multielemental Characterization of Bixa orellana L. (Urucum) Seeds by Inductively Coupled Plasma Mass Spectrometry10.1007/s11947-023-03073-6 ↗
11. Ferreira, W.d.A., and Falesi, I.C. (1989). Características Nutricionais Do Fruto E Teor De Bixina Em Urucu (Bixa orellana L.), Embrapa. Available online: https://www.embrapa.br/busca-de-publicacoes/-/publicacao/381124/caracteristicas-nutricionais-do-fruto-e-teor-de-bixina-em-urucu-bixa-orellana-l.
12. Sulphur in Crop Production-Invited Paper10.1016/S1161-0301(00)00082-4 ↗
13. Variation in Sulfur and Selenium Accumulation Is Controlled by Naturally Occurring Isoforms of the Key Sulfur Assimilation Enzyme Adenosine 5′-Phosphosulfate REDUCTASE2 across the Arabidopsis Species Range10.1104/pp.114.247825 ↗
14. Sulfur: Not a “Silent” Element Any More10.1039/B417595F ↗
15. Elemental Concentration and Sulfur Chemical Speciation in the Amazonian Plant Andira Surinamensis Using Synchrotron Radiation Techniques (SR-XRF, XANES), RBS and WD-XRF
16. Gonzalez, J.C. (2019). Uso de Técnicas Espectroscópicas Avançadas Para Caracterização Elementar e Especiação de Enxofre Em Plantas Da Amazônia. [Ph.D. Thesis, Federal University of Rio de Janeiro].
17. Bruker (2009). S8 Tiger Operator’s Manual, Bruker.
18. Jenkins, R. (2008). X-ray Fluorescence Spectrometry, RSC Publishing.
19. Haschke, M. (2014). Micro-X-ray Fluorescence Spectroscopy, Springer.10.1007/978-3-319-04864-2 ↗
20. Quantitative X-ray Fluorescence Analysis of Biomass: Objective Evaluation of a Typical Commercial Multi-Element Method on a WD-XRF Spectrometer10.1021/ef4015394 ↗
21. The Soft X-ray Spectroscopy Beamline at the LNLS: Technical Description and Commissioning Results10.1107/S0909049599008122 ↗
22. Commissioning and First Results of the LNLS XAFS Beamline10.1107/S0909049597015264 ↗
23. ATHENA, ARTEMIS, HEPHAESTUS: Data Analysis for X-ray Absorption Spectroscopy Using IFEFFIT10.1107/S0909049505012719 ↗
24. A NEXAFS and Mass Spectrometry Study of Cysteine, Cystine and Insulin Irradiated with Intermediate Energy (0.8 KeV) Electrons10.1016/j.elspec.2014.02.002 ↗
25. Uptake of Macro Nutrients, Barium, and Strontium by Vegetation from Mineral Soils on Carbonatite and Pyroxenite Bedrock at the Lillebukt Alkaline Complex on Stjernøy, Northern Norway10.1002/jpln.201600328 ↗
26. Sulfur Speciation in Heavy Petroleums: Information from X-ray Absorption near-Edge Structure10.1016/0016-7037(91)90343-4 ↗
27. Using X-ray Absorption to Probe Sulfur Oxidation States in Complex Molecules10.1016/S1386-1425(98)00153-X ↗
28. XANES Studies of Oxidation States of Sulfur in Aquatic and Soil Humic Substances10.2136/sssaj1998.03615995006200050014x ↗
29. Levels of Inorganic Constituents in Raw Nuts and Seeds on the Swedish Market10.1016/j.scitotenv.2007.11.024 ↗
30. Almeida, I.M.C., Oliva-Teles, M.T., Alves, R.C., Santos, J., Pinho, R.S., Silva, S.I., Delerue-Matos, C., and Oliveira, M.B.P.P. (2020). Oilseeds from a Brazilian Semi-Arid Region: Edible Potential Regarding the Mineral Composition. Foods, 9.10.3390/foods9020229 ↗
31. Functional Packaging of Seeds10.1111/nph.17299 ↗
32. Seed Coat Composition in Black and White Soybean Seeds with Differential Water Permeability10.1111/plb.13551 ↗
33. FAO, and WHO (2024, June 08). CF/14 INF/1. Joint FAO/WHO Food Standards Program CODEX Committee on Contaminants in Foods. Available online: https://www.fao.org/fao-who-codexalimentarius/sh-proxy/it/?lnk=1&url=https%253A%252F%252Fworkspace.fao.org%252Fsites%252Fcodex%252FMeetings%252FCX-735-14%252FINFO-DOC%252FCF14_INF01x.pdf.
34. Sulfur K-Edge X-ray Absorption Spectroscopy of Petroleum Asphaltenes and Model Compounds10.1021/ja00191a012 ↗
35. Quantitative Analysis of All Major Forms of Sulfur in Coal by X-ray Absorption Fine Structure Spectroscopy10.1021/ef00028a008 ↗
36. Determination of the Chemical Environment of Sulphur in Petroleum Asphaltenes by X-ray Absorption Spectroscopy10.1016/0016-2361(92)90192-Q ↗
37. Sulfur Speciation of Desulfurized Coals by XANES Spectroscopy10.1016/0016-2361(95)98358-L ↗
38. Speciation and Quantification of Sulfur Compounds in Petroleum Asphaltenes by Derivative XANES Spectra10.1016/S1872-5813(14)60002-5 ↗
39. Characterization of Sulfur-Containing Functional Groups in Sedimentary Humic Substances by X-ray Absorption near-Edge Structure Spectroscopy10.1021/ef960212a ↗
40. Principal Component Analysis Approach for Modeling Sulfur K-XANES Spectra of Humic Acids
41. Combining Selective Sequential Extractions, X-ray Absorption Spectroscopy, and Principal Component Analysis for Quantitative Zinc Speciation in Soil10.1021/es025669f ↗
42. Sulfur K-Edge XANES Spectroscopy as a Tool for Understanding Sulfur Dynamics in Soil Organic Matter10.2136/sssaj2003.1721 ↗
43. Record of Redox Status in Laminated Sediments from Lake Titicaca: A Sulfur K-Edge X-ray Absorption near Edge Structure (XANES) Study10.1016/j.chemgeo.2005.02.004 ↗
44. Sulphur Speciation and Turnover in Soils: Evidence from Sulphur K-Edge XANES Spectroscopy and Isotope Dilution Studies10.1016/j.soilbio.2005.08.013 ↗
45. Sulfur Speciation in Soil by S K-Edge XANES Spectroscopy: Comparison of Spectral Deconvolution and Linear Combination Fitting10.1021/es102180a ↗
46. Sulfur K-Edge X-ray Absorption Spectroscopy for Determining the Chemical Speciation of Sulfur in Biological Systems10.1016/S0014-5793(98)01402-1 ↗
47. Sulfur K-Edge x-Ray Absorption Spectroscopy: A Spectroscopic Tool to Examine the Redox State of S-Containing Metabolites in Vivo10.1073/pnas.95.11.6122 ↗
48. In Situ Analysis of Sulfur in the Sulfur Globules of Phototrophic Sulfur Bacteria by X-ray Absorption near Edge Spectroscopy10.1016/S0304-4165(99)00095-1 ↗
49. X-ray Absorption near-Edge Structure (XANES) Spectroscopy Identifies Differential Sulfur Speciation in Corneal Tissue10.1007/s00216-013-7120-x ↗
50. Pereira, A., Tavares, P., and Limão-Vieira, P. (2019). Near Edge X-ray Absorption Fine Structure (NEXAFS) Spectroscopy of Molecules of Biological Interest: From Chemically Pure to Complex Samples. Radiation in Bioanalysis, Springer Nature.10.1007/978-3-030-28247-9 ↗
51. EXAFS and Principal Component Analysis: A New Shell Game10.1107/S0909049599000965 ↗
52. Sulfur K-Edge XAS and DFT Calculations on P450 Model Complexes: Effects of Hydrogen Bonding on Electronic Structure and Redox Potentials10.1021/ja0519031 ↗
53. When Identical Functional Groups Are Not Identical: A DFT Study of the Effects of Molecular Environment on Sulfur K-Edge X-ray Absorption Spectra10.1016/j.ica.2007.05.047 ↗
54. K-Edge XANES Analysis of Sulfur Compounds: An Investigation of the Relative Intensities Using Internal Calibration10.1107/S0909049510022946 ↗
55. Sulfur 1s Near-Edge X-ray Absorption Fine Structure (NEXAFS) of Thiol and Thioether Compounds10.1063/1.3602218 ↗
56. Chemical Sensitivity of the Sulfur K-Edge X-ray Absorption Spectra of Organic Disulfides10.1021/acs.jpca.6b06790 ↗
57. Maturation of Arabidopsis Seeds Is Dependent on Glutathione Biosynthesis within the Embryo10.1104/pp.106.077982 ↗
58. Mondal, S., Pramanik, K., Panda, D., Dutta, D., Karmakar, S., and Bose, B. (2022). Sulfur in Seeds: An Overview. Plants, 11.10.3390/plants11030450 ↗
59. Transporter Gene Families in Plants: The Sulphate Transporter Gene Family—Redundancy or Specialization?10.1034/j.1399-3054.2003.00034.x ↗
60. Amino Acid, Fatty Acid, and Mineral Composition of 24 Indigenous Plants of Burkina Faso10.1006/jfca.1997.0539 ↗