journal article·2023-11-15Open access

AI Classification of Eggs’ Origin from Mycoplasma synoviae-Infected or Non-Infected Poultry via Analysis of the Spectral Response

Anna Pakuła, Sławomir Paśko, Paweł Marć, Olimpia Kursa, Leszek R. Jaroszewicz

Applied Sciences· Volume 13· Issue 22

Abstract

Rapid detection of Mycoplasma synoviae (MS) in a flock is crucial from the perspective of animals’ health and economic income. MS are highly contagious bacteria that can cause significant losses in commercial poultry populations when its prevalence is not limited. MS infections can cause losses associated with a range of clinical symptoms related to the respiratory, mobility and reproductive systems. Lesions related to the reproductive system and changes in the eggshell result in losses associated with infection or embryo death or complete destruction of the eggs. The authors propose using spectral measurements backed up by an AI data processing algorithm to classify eggs’ origin: from healthy hens or MS-infected ones. The newest obtained classification factors are F-scores for white eggshells of 99% and scores for brown eggshells of 99%—all data used for classification were obtained using a portable multispectral fibre-optics reflectometer. The proposed method may be used directly on the farm by staff members with limited qualifications, as well as by veterinary doctors, assistants, or customs officers. Moreover, this method is scalable to mass production of eggs. Standard methods such as serological tests require either specialized staff or laboratory equipment. Implementation of a non-destructive optical measurement method, which is easily adapted for use on a production line, is economically reasonable.
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Research context

OpenAlex topics: Microbial infections and disease research · Animal Behavior and Welfare Studies · Mycotoxins in Agriculture and Food

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institute of Micromechanics and Photonics, Warsaw University of Technology, ul. Św. A. Boboli 8, 02-525 Warsaw, Poland

Faculty of New Technologies and Chemistry, Military University of Technology, ul. gen. Sylwestra Kaliskiego 2, 00-908 Warsaw, Poland

Department of Poultry Diseases, National Veterinary Research Institute, Al. Partyzantów 57, 24-100 Puławy, Poland

Funding

Military University of Technology · Institute of Micromechanics and Photonics statutory

References (53)

Show source-provided references
1. Induction of eggshell apex abnormalities by Mycoplasma synoviae: Field and experimental studies10.1080/03079450802662772 ↗
2. Kursa, O., Pakuła, A., Tomczyk, G., Paśko, S., and Sawicka, A. (2019). Eggshell apex abnormalities caused by two different Mycoplasma synoviae genotypes and evaluation of eggshell anomalies by full-field optical coherence tomography. BMC Vet. Res., 15.10.1186/s12917-018-1758-8 ↗
3. Treatment of Eggshell Abnormalities and Reduced Egg Production Caused by Mycoplasma Synoviae Infection10.1637/9121-110309-Case.1 ↗
4. The Effect of Eggshell Apex Abnormalities on Table Egg Quality during Storage in 2 Seasons of the Year10.3382/ps.2014-03991 ↗
5. Effect of Mycoplasma Synoviae Strains Isolated from the Respiratory and Reproductive Tracts of Chicken on SPF Chicken Embryos
6. Research on Eggshell Structure and Quality: An Historical Overview10.1590/S1516-635X2005000200001 ↗
7. New rapid enzyme-linked immunosorbent assay to detect antibodies against bacterial surface antigens using filtration plates10.1248/bpb.25.986 ↗
8. Specific detection of Salmonella enterica and Escherichia coli strains by using ELISA with bacteriophages as recognition agents10.1007/s10096-011-1193-2 ↗
9. Combined optical coherence tomography and spectral technique for detection of changes in eggshells caused by Mycoplasma synoviae
10. Seroprevalence of Mycoplasma synoviae in Dutch commercial poultry farms10.1080/03079450802484987 ↗
11. Mycoplasmas in the etiology of multifactorial respiratory disease10.1093/ps/77.8.1146 ↗
12. Pathogen detection: A perspective of traditional methods and biosensors10.1016/j.bios.2006.06.036 ↗
13. Specific detection and typing of Mycoplasma synoviae strains in poultry with PCR and DNA sequence analysis targeting the hemagglutinin encoding gene vlhA10.1637/7156-011504R ↗
14. Development and evaluation of an improved diagnostic PCR for Mycoplasma synoviae using primers located in the haemagglutinin encoding gene vlhA and its value for strain typing10.1016/j.vetmic.2008.10.011 ↗
15. The development of diagnostic real-time TaqMan PCRs for the four pathogenic avian mycoplasmas10.1637/8469-091508-Reg.1 ↗
16. A multiplex real-time PCR for detection of Mycoplasma gallisepticum and Mycoplasma synoviae in clinical samples from Brazilian commercial poultry flocks10.1590/S1517-83822013000200028 ↗
17. Rapid detection of Mycoplasma synoviae by loop-mediated isothermal amplification10.1007/s00203-014-1063-2 ↗
18. Prevalence and phylogenetic analysis of Mycoplasma synoviae strains isolated from Polish chicken layer flocks10.2478/jvetres-2019-0010 ↗
19. Deep Learning Spectroscopy: Neural Networks for Molecular Excitation Spectra10.1002/advs.201801367 ↗
20. Crase, S., and Thennadil, S.N. (2022). An analysis framework for clustering algorithm selection with applications to spectroscopy. PLoS ONE, 17.10.1371/journal.pone.0266369 ↗
21. Artificial Intelligence in Analytical Spectroscopy, Part II: Examples in Spectroscopy10.56530/spectroscopy.js8781e3 ↗
22. Trends in Artificial Intelligence, Machine Learning, and Chemometrics Applied to Chemical Data10.1002/ansa.202000162 ↗
23. Advances in Infrared Spectroscopy and Hyperspectral Imaging Combined with Artificial Intelligence for the Detection of Cereals Quality10.1080/10408398.2022.2066062 ↗
24. Opportunities of Artificial Intelligence and Machine Learning in the Food Industry10.1155/2021/4535567 ↗
25. An attempt to classify the botanical origin of honey using visible spectroscopy
26. Spectral technique for detection of changes in eggshells caused by Mycoplasma synoviae10.3382/ps/pez150 ↗
27. Szewczyk, R., Krejsa, J., Nowicki, M., and Ostaszewska-Liżewska, A. (2020). Advances in Intelligent Systems and Computing 1044, Proceedings of the Mechatronics 2019—Computing in Mechatronics, Warsaw, Poland, 16–18 September 2019, Springer.
28. Pakuła, A., Żołnowski, W., Paśko, S., Kursa, O., Marć, P., and Jaroszewicz, L.R. (2022). Multispectral Portable Fibre-Optic Reflectometer for the Classification of the Origin of Chicken Eggshells in the Case of Mycoplasma synoviae Infections. Sensors, 22.10.3390/s22228690 ↗
29. Exploration of Principal Component Analysis: Deriving Principal Component Analysis Visually Using Spectra10.1177/0003702820987847 ↗
30. In Situ Detection of Live-to-Dead Bacteria Ratio After Inactivation by Means of Synchronous Fluorescence and PCA10.1073/pnas.1716514115 ↗
31. Kharbach, M., Alaoui Mansouri, M., Taabouz, M., and Yu, H. (2023). Current Application of Advancing Spectroscopy Techniques in Food Analysis: Data Handling with Chemometric Approaches. Foods, 12.10.3390/foods12142753 ↗
32. Data fusion and multivariate analysis for food authenticity analysis10.1038/s41467-023-38382-z ↗
33. Pakuła, A., Paśko, S., Kursa, O., and Komar, R. (2021). Reflected Light Spectrometry and AI-Based Analysis for Detection of Rapid Chicken Eggshell Change Caused by Mycoplasma Synoviae. Appl. Sci., 11.10.3390/app11177799 ↗
34. Deep Learning Optical Spectroscopy Based on Experimental Database: Potential Applications to Molecular Design10.1021/jacsau.1c00035 ↗
35. Ting, H.-W., Chung, S.-L., Chen, C.-F., Chiu, H.-Y., and Hsieh, Y.-W. (2020). A drug identification model developed using deep learning technologies: Experience of a medical center in Taiwan. BMC Health Serv. Res., 20.10.1186/s12913-020-05166-w ↗
36. Support-vector networks10.1007/BF00994018 ↗
37. RBF kernel based support vector machine with universal approximation and its application
38. Bharadwaj, K., Prakash, B., and Kanagachidambaresan, G.R. (2021). Pattern Recognition and Machine Learning, Springer. Chapter 7.10.1007/978-3-030-57077-4_11 ↗
39. Phylogenetic reconstruction using an unsupervised growing neural network that adopts the topology of a phylogenetic tree10.1007/PL00006139 ↗
40. Fundamental Overview of SOTA-Ensemble Learning Methods for Deep Learning: A Systematic Literature Review10.31763/sitech.v2i2.549 ↗
41. Multigene Families Encoding the Major Hemagglutinins in Phylogenetically Distinct Mycoplasmas10.1128/IAI.66.7.3470-3475.1998 ↗
42. Molecular Basis of the Length Variation in the N-Terminal Part of Mycoplasma Synoviae Hemagglutinin10.1111/j.1574-6968.2001.tb10829.x ↗
43. Swayne, D.E., Glisson, J.R., McDougald, L.R., Nolan, L.K., Suarez, D.L., and Nair, V.L. (2013). Diseases of Poultry, Wiley.
44. Mycoplasma Synoviae Has Two Distinct Phase-Variable Major Membrane Antigens, One of Which Is a Putative Hemagglutinin10.1128/iai.65.7.2542-2547.1997 ↗
45. The Hemagglutination-Positive Phenotype of Mycoplasma Synoviae Induces Experimental Infectious Synovitis in Chickens More Frequently than Does the Hemagglutination-Negative Phenotype10.1128/IAI.66.12.6004-6009.1998 ↗
46. Mycoplasma Gallisepticum and Mycoplasma Synoviae Express a Cysteine Protease CysP, Which Can Cleave Chicken IgG into Fab and Fc10.1099/mic.0.045641-0 ↗
47. Sialidase Activity in Mycoplasma Synoviae10.1637/7806-120106-REGR.1 ↗
48. Diversity of Expressed VlhA Adhesin Sequences and Intermediate Hemagglutination Phenotypes in Mycoplasma Synoviae10.1128/JB.00022-11 ↗
49. Phenotypic Diversity in an Emerging Mycoplasmal Disease10.1016/j.micpath.2019.103798 ↗
50. Infection Strategies of Mycoplasmas: Unraveling the Panoply of Virulence Factors10.1080/21505594.2021.1889813 ↗
51. Neuraminidase of Mycoplasma Synoviae Desialylates Heavy Chain of the Chicken Immunoglobulin G and Glycoproteins of Chicken Tracheal Mucus10.1080/03079457.2011.565311 ↗
52. Demonstration of Neuraminidase Activity in Mycoplasma Neurolyticum and of Neuraminidase Proteins in Three Canine Mycoplasma Species10.1016/j.vetmic.2011.08.026 ↗
53. Resistance to Mycoplasma Synoviae Is Bursal Dependent10.1128/iai.8.4.674-676.1973 ↗