Protoporphyrin IX Based All-Solid-State Ion-Selective Electrodes for Choline Determination In Vitro
Source abstract
Abstract
Protoporphyrin IX-based all-solid-state choline (Ch) ion-selective electrodes (ISEs) were fabricated and characterized. Poly (3,4-ethylene dioxythiophene) doped with poly (styrene sulfonate) (PEDOT/PSS) functioning as an ion-to-electron transducer was electropolymerized on the gold wire (0.5 mm diameter). The conductive polymer was covered with a Ch selective membrane containing protoporphyrin IX as an ionophore, which exhibited a lower detection limit of 0.49 μM with the potentiometric method. The Ch sensor performed a wide linear range from 1 μM to 1 mM, a fast response time of less than 5 s, and a decent selectivity of common inorganic and organic ions in the human body. Characteristics such as pH and temperature stability, life span, reproducibility and repeatability were also investigated to be satisfied. With the background of artificial cerebrospinal fluid, the recovery rate in 10−5 M of Ch solution was measured by the standard addition method, revealing the potential for biological application.
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Research context
OpenAlex topics: Analytical Chemistry and Sensors · Electrochemical sensors and biosensors · Electrochemical Analysis and Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
State Key Laboratory of Industrial Control Technology, College of Control Science and Engineering, Zhejiang University, Hangzhou 310027, China
Computer Learning Research Centre, Computer Science Department, Royal Holloway University of London, Surrey TW20 0EX, UK
Funding
National Natural Science Foundation of China · Autonomous Research Project of the State Key Laboratory of Industrial Control 242 Technology, China
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