DataVXApplied SciencesPaper
journal article·2021-05-19Open access

Generation of Monoclonal Antibodies for Sensitive Detection of Pro-Inflammatory Protein S100A9

Eun-Jung Kim, Gyu-Min Im, Chang-Soo Lee, Yun-Gon Kim, Byoung Joon Ko, Hee-Jin Jeong, Byung-Gee Kim

Applied Sciences· Volume 11· Issue 10
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Source abstract

Abstract

The calcium-binding protein S100A9 regulates inflammatory processes and the immune response. It is overexpressed in a variety of inflammatory and oncologic conditions. In this study, we produced a recombinant human S100A9 (hS100A9) antigen with high yield and purity and used it to generate a hybridoma cell culture-based monoclonal anti-hS100A9 antibody. We selected five anti-hS100A9 antibodies from cell supernatants that showed high antigen binding efficiency and identified the nucleotide sequences of three antibodies: two with high effective concentration values and one with the lowest value. The antigen and antibody development procedures described herein are useful for producing large amounts of monoclonal antibodies against hS100A9 and other antigens of interest. The nucleotide sequences of the anti-hS100A9 monoclonal antibody revealed herein will be helpful in the generation of recombinant antibodies or antibody fragments against hS100A9.
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Research context

OpenAlex topics: S100 Proteins and Annexins · Protease and Inhibitor Mechanisms · Cell Adhesion Molecules Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Bio-MAX/N-Bio, Seoul National University, Seoul 08826, Korea

Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University, Seoul 08826, Korea

Department of Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 34134, Korea

Department of Chemical Engineering, Soongsil University, Seoul 06978, Korea

School of Biopharmaceutical and Medical Sciences, Sungshin Women’s University, Seoul 02844, Korea

Department of Biological and Chemical Engineering, Hongik University, Sejong 30016, Korea

Funding

National Research Foundation of Korea (NRF) grant funded by Ministry of Science, ICT · NRF grant funded by the Ministry of Science, ICT & Future Planning · Industrial Strategic technology development program funded By the Ministry of Trade, industry & Energy · NRF grant funded by the Korean government

References (14)

Show source-provided references
1. S100A8/A9 in Inflammation10.3389/fimmu.2018.01298 ↗
2. Neutrophil-Derived MRP14 Supports Plasma Cell Commitment and Protects Myeloma Cells from Apoptosis
3. Review of S100A9 biology and its role in cancer
4. Co-aggregation of pro-inflammatory S100A9 with α-synuclein in Parkinson’s disease: Ex vivo and in vitro studies10.1186/s12974-018-1210-9 ↗
5. S100 Proteins in Alzheimer’s Disease10.3389/fnins.2019.00463 ↗
6. The role of pro-inflammatory S100A9 in Alzheimer’s disease amyloid-neuroinflammatory cascade10.1007/s00401-013-1208-4 ↗
7. S100B is increased in Parkinson’s disease and ablation protects against MPTP-induced toxicity through the RAGE and TNF-α pathway10.1093/brain/aws250 ↗
8. Allgöwer, C., Kretz, A.-L., Von Karstedt, S., Wittau, M., Henne-Bruns, D., and Lemke, J. (2020). Friend or Foe: S100 Proteins in Cancer. Cancers, 12.10.3390/cancers12082037 ↗
9. Aberrant MRP14 expression in thyroid follicular cells mediates chemokine secretion through the IL-1β/MAPK pathway in Hashimoto’s thyroiditis10.1530/EC-18-0019 ↗
10. The subcellular distribution of myeloid-related protein 8 (MRP8) and MRP14 in human neutrophils10.1186/1479-5876-3-36 ↗
11. Phagocyte-specific S100A8/A9 Protein Levels during Disease Exacerbations and Infections in Systemic Lupus Erythematosus10.3899/jrheum.081302 ↗
12. Phagocyte-specific calcium-binding S100 proteins as clinical laboratory markers of inflammation10.1016/j.cccn.2004.02.023 ↗
13. Continuous cultures of fused cells secreting antibody of predefined specificity
14. Simple and inexpensive three-step rapid amplification of cDNA 5′ ends using 5′ phosphorylated primers10.1016/j.ab.2012.10.031 ↗