MiR-486-3p and MiR-938—Important Inhibitors of Pacemaking Ion Channels and/or Markers of Immune Cells
Source abstract
Abstract
The sinus node (SN) is the heart’s primary pacemaker and has a unique expression of pacemaking ion channels and immune cell markers. The role of microribonucleic acids (miRNAs) in control of ion channels and immune function of the sinus node is not well understood. We have recently shown that hsa-miR-486-3p downregulates the main pacemaking channel HCN4 in the SN. In addition, we recently demonstrated that immune cells are significantly more abundant in the SN compared to the right atrium. The aim of this study was to validate the previously predicted interactions between miRNAs and mRNAs of key Ca2+ ion channels (involved in peacemaking) and mRNA of TPSAB1—(a mast cells marker) using luciferase assay. We now show that miR-486 significantly downregulates Cav1.3, Cav3.1, and TPSAB1-mediated luciferase activity, while miR-938 significantly downregulates only TPSAB1-mediated luciferase activity. This makes miR-486-3p a potential therapeutic target in the treatment of SN dysfunctions.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Viral Infections and Immunology Research · Traditional Chinese Medicine Analysis · Cardiac Fibrosis and Remodeling
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Cardiovascular Sciences, School of Medicine, University of Manchester, Manchester M13 9PL, UK
Biological Faculty, Department of Human and Animal Physiology, Leninskie Gory 1, Building 2, Lomonosov Moscow State University, 119991 Moscow, Russia
Department of Anatomy, Jagiellonian University Medical College, Świętej Anny 12, 31-008 Krakow, Poland
Funding
British Heart Foundation · Fondation Leducq
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