Imaging of Cell Structures Using Optimized Soft X-ray Contact Microscopy
Source abstract
Abstract
This work is to study the relationship between the exposure conditions and the quality of cell imaging with soft X-ray contact microscopy (SXCM). It is a crucial step in the efficient visualization of cell structures. Three different human cell lines: DU145 prostate carcinoma cells, HCC38 breast cancer cells, and Poietics mesenchymal stem cells were used to establish the optimal exposure conditions in SXCM. The image quality depended on the soft X-ray (SXR) absorbed energy and photoresist development conditions. At lower SXR energy (200 or 400 SXR pulses), sharp cell edges, membrane projections, and cell–cell connections were visible. In contrast, higher energy (600 or 800 SXR pulses) allowed observation of the cytoskeleton and the nucleus in a cell type-dependent manner (the influence of cell thickness and internal complexity was noted).
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Research context
OpenAlex topics: Advanced X-ray Imaging Techniques · Advanced Electron Microscopy Techniques and Applications · Advanced Fluorescence Microscopy Techniques
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Biomedical Engineering Centre, Institute of Optoelectronics, Military University of Technology, PL-00908 Warsaw, Poland
Laser Technology Division, Institute of Optoelectronics, Military University of Technology, PL-00908 Warsaw, Poland
Institute of Nuclear Physics, Polish Academy of Sciences, PL-31342 Krakow, Poland
Funding
Narodowe Centrum Nauki · European Union under the Horizon 2020 Programme Laserlab-Europe Project
References (35)
Show source-provided references
1. Correlative imaging of live biological cells with a soft x-ray microscope and a fluorescence microscope10.1063/1.4937513 ↗
2. Contact X-ray microscopy of living cells by using LiF crystal as imaging detector10.1111/jmi.12226 ↗
3. Scanning electron microscopy as a new tool for diagnostic pathology and cell biology10.1016/j.micron.2019.102797 ↗
4. A quick guide to light microscopy in cell biology10.1091/mbc.e15-02-0088 ↗
5. Application of X-ray microscopy in analysis of living hydrated cells10.1002/ar.10166 ↗
6. Study of Multicellular Living Organisms by SXCM (Soft X-Ray Contact Microscopy)10.4028/www.scientific.net/SSP.107.7 ↗
7. In situ observation of cellular organelles with a contact x-ray microscope10.1088/1742-6596/463/1/012056 ↗
8. Nanoscale X-ray imaging10.1038/nphoton.2010.267 ↗
9. Winick, H., and Doniach, S. (1980). Soft X-Ray Microscopy of Biological Specimens. Synchrotron Radiation Research, Springer.10.1007/978-1-4615-7998-4 ↗
10. Study on resist sensitivities for nano-scale imaging using water window X-ray microscopy10.1016/j.radphyschem.2010.07.041 ↗
11. Soft X-ray microscopes and their biological applications10.1017/S0033583500003139 ↗
12. Development and characterization of a laser-plasma soft X-ray source for contact microscopy10.1016/j.nimb.2017.03.082 ↗
13. Wachulak, P., Torrisi, A., Ayele, M., Czwartos, J., Bartnik, A., Wegrzynski, L., Fok, T., Parkman, T., Salacova, S., and Turňová, J. (2017). Bioimaging Using Full Field and Contact EUV and SXR Microscopes with Nanometer Spatial Resolution. Appl. Sci., 7.10.3390/app7060548 ↗
14. Liquid cell transmission electron microscopy and its applications10.1098/rsos.191204 ↗
15. Transmission microscopy of unmodified biological materials. Comparative radiation dosages with electrons and ultrasoft X-ray photons10.1016/S0304-3991(76)91997-5 ↗
16. Development and application of STEM for the biological sciences10.1016/j.ultramic.2012.04.005 ↗
17. X-ray microscopy with synchrotron radiation10.1038/1341 ↗
18. High-resolution compact X-ray microscopy10.1111/j.1365-2818.2007.01765.x ↗
19. Optimization of a laser-plasma x-ray source for contact x-ray microscopy10.1063/1.356055 ↗
20. Laser plasma interaction in solid metal, mixed metal alloy and metal nano-particle coated targets10.1088/1742-6596/112/3/032050 ↗
21. Soft x-ray microscope constructed with a PMMA phase-reversal zone plate10.1364/OL.34.000235 ↗
22. Soft X-ray contact microscopy of nematode Caenorhabditis elegans
23. Sayre, D., Kirz, J., Howells, M., and Rarback, H. (1988). High Resolution Image Storage in Polymers, X-Ray Microscopy II, Springer.10.1007/978-3-540-39246-0 ↗
24. Future challenges for x-ray microscopy10.1063/1.4937529 ↗
25. Imaging cell morphology and physiology using X-rays10.1042/BST20180036 ↗
26. Resolution limits for electron-beam lithography10.1147/rd.324.0502 ↗
27. Control of cell adhesion on poly(methyl methacrylate)10.1016/j.biomaterials.2005.12.009 ↗
28. Improvement of PMMA electron-beam lithography performance in metal liftoff through a poly-imide bi-layer system10.1016/j.mee.2010.07.030 ↗
29. Low stress development of poly(methylmethacrylate) for high aspect ratio structures
30. Fabrication of 3D nanoimprint stamps with continuous reliefs using dose-modulated electron beam lithography and thermal reflow10.1088/0960-1317/20/9/095002 ↗
31. Comparison of MIBK/IPA and water/IPA as PMMA developers for electron beam nanolithography10.1016/S0167-9317(02)00468-9 ↗
32. Nanoscale resist morphologies of dense gratings using electron-beam lithography
33. The effects of molecular weight on the exposure characteristics of poly(methylmethacrylate) developed at low temperatures
34. Human prostate DU145 carcinoma cells implanted in nude mice remove the peritoneal mesothelium to invade and grow as carcinomas10.1002/ar.22607 ↗
35. Correlation between proliferative activity and cellular thickness of human mesenchymal stem cells10.1016/j.bbrc.2008.01.051 ↗