Open-Source Tools for Volume Estimation of 3D Multicellular Aggregates
Source abstract
Abstract
The volume is one of the most relevant features that define the treatment of an in vivo tumour. When using cancer 3D in vitro models in pre-clinical studies, it becomes important to evaluate the macroscopic effects of drugs and radiotherapy treatments. Depending on the nature of the 3D in vitro model used, different open-source solutions can be used for measuring the volume by starting from microscope-acquired images. In this work, we introduced several open-source tools today available for estimating the volume of 3D multicellular aggregates (e.g., spheroids, organoids), also giving hints for defining the “best software” by analysing characteristics of 3D in vitro models and limits of the tools. Finally, using several cancer organoids imaged by a fluorescent microscope, we compared volume estimations obtained with different tools, besides presenting a new version of the Reconstruction and Visualization from Multiple Sections (ReViMS version 2.0) tool. This work aims to be the reference for researchers interested in estimating the volume of 3D multicellular aggregates through an open-source tool.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: 3D Shape Modeling and Analysis · 3D Printing in Biomedical Research · Cell Image Analysis Techniques
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Advanced Research Center on Electronic Systems (ARCES) for Information and Communication Technologies “E. De Castro”, University of Bologna, I-40125 Bologna, Italy
Interdepartmental Centre “L. Galvani” for Integrated Studies of Bioinformatics, Biophysics & Biocomplexity (CIG), University of Bologna, I-40125 Bologna, Italy
Synthetic and System Biology Unit, Hungarian Academy of Sciences, Biological Research Centre (BRC), H-6726 Szeged, Hungary
Institute for Molecular Medicine Finland, University of Helsinki, FI-00014 Helsinki, Finland
Department of Computer Science and Engineering (DISI), University of Bologna, I-40126 Bologna, Italy
Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, I-47014 Meldola (FC), Italy
Funding
NEUBIAS COST Action (European Cooperation in Science and Technology) CA15124 · HAS-LENDULET-BIOMAG and EUROPEAN UNION and EUROPEAN REGIONAL DEVELOPMENT FUNDS
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