Effects of Various Extraction Factors on Protein Yield of Haliotis discus hannai (Abalone)
Source abstract
Abstract
Abalone (Haliotis discus hannai) is a widely consumed seafood in Asian countries. Rich in protein, abalone is consumed for refreshment, pregnancy care, and vitality. Although many studies have found that abalone protein has beneficial effects, the efficiency of the protein extraction method for abalone has rarely been studied. Therefore, this study aimed to examine the effects of various factors of abalone protein extraction, including extraction buffer, sonication, salt (NaCl) concentration, surfactant, and heating. Phosphate buffer showed higher protein yield compared with Tris-HCl buffer. In addition, the highest protein yield for each factor was observed at 60 s of sonication (84.44 µg/mg dw), 0.6 M NaCl (141.9 µg/mg dw), and 16 mM sodium dodecyl sulfate (SDS) (253.15 µg/mg dw). However, a combined effect was not observed. Lower protein extraction efficiency was observed for sous vide-cooked abalone. The electrophoresis assay revealed myofibrillar proteins, including paramyosin, actin, and tropomyosin. Overall, our results demonstrate that various extract conditions affect the protein extraction of abalone.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Meat and Animal Product Quality · Protein Hydrolysis and Bioactive Peptides · Food Industry and Aquatic Biology
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Smart Green Technology Engineering, Pukyong National University, Busan 48513, Korea
Food Safety and Processing Research Division, National Institute Fisheries Science, Busan 46083, Korea
Department of Food Science and Nutrition, Pukyong National University, Busan 48513, Korea
Department of Food Science and Nutrition, Pusan National University, Busan 46241, Korea
Funding
Future fisheries food research center, the Ministry of Oceans and Fisheries, Republic of Korea · National Institute of Fisheries Science (NIFS), Republic of Korea
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