Effect of Bioaugmentation with Anaerobic Fungi Isolated from Ruminants on the Hydrolysis of Corn Silage and Phragmites australis
Source abstract
Abstract
Anaerobic fungi produce extracellular hydrolytic enzymes that facilitate degradation of cellulose and hemicellulose in ruminants. The purpose of this work was to study the impact of three different anaerobic fungal species (Anaeromyces mucronatus YE505, Neocallimastix frontalis 27, and Piromyces rhizinflatus YM600) on hydrolysis of two different lignocellulosic substrates, corn (Zea mays L.) silage and reed (Phragmites australis (Cav.) Trin. ex Steud.). Biomass from each plant species was incubated anaerobically for 11 days either in the presence of live fungal inoculum or with heat-inactivated (control) inoculum. Headspace gas composition, dry matter loss, soluble chemical oxygen demand, concentration of volatile fatty acids, and chemical composition were measured before and after hydrolysis. While some microbial activity was observed, inoculation with anaerobic fungi did not result in any significant difference in the degradation of either type of plant biomass tested, likely due to low fungal activity or survival under the experimental conditions tested. While the premise of utilizing the unique biological activities of anaerobic fungi for biotechnology applications remains promising, further research on optimizing culturing and process conditions is necessary.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Biofuel production and bioconversion · Anaerobic Digestion and Biogas Production · Bioenergy crop production and management
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
School of Environmental Sciences, University of Guelph Ridgetown Campus, Ridgetown, ON N0P 2C0, Canada
Agriculture and Agri-Food Canada, Lethbridge Research Station, Lethbridge, AB T1J 4P4, Canada
School of Environmental Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada
Funding
Natural Resources Canada · Agriculture and Agri-Food Canada · Natural Sciences and Engineering Research Council of Canada
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