Cytokine-Related Effect of Buccal-Delivered Collagen Peptide Incorporated in Mucoadhesive Films to Improve Female Skin Conditions
Source abstract
Abstract
Recently, interest in collagen products has increased in the industries However, collagen products that are taken orally have the problem of being degraded by digestive enzymes. Therefore, a collagen peptide buccal delivery film (C-BDF) was developed to enhance the absorption without destruction and a clinical trial was conducted. A C-BDF was developed as a double layer and the permeation of collagen peptide (CP) through swine mucosa was investigated. This clinical study was performed on 43 healthy women, who were divided into either a control (n = 21) or test group (n = 22), over the course of 4 weeks. Skin assessments analyzed the hydration, elasticity, and roughness. In addition, the production of peroxynitrite and IL-1α in RAW 264.7 cells in supernatant media was conducted. A total of 1 kDa of CP in BDF showed significantly stronger permeation through swine mucosa compared to 3 kDa of CP in BDF. The C-BDF significantly enhanced skin hydration, elasticity, and roughness, and it removed wrinkles with no side effects after 2 weeks of intake. In addition, the production of peroxynitrite and IL-1α after the treatment with CP was significantly increased. Therefore, this study showed that collagen peptides could be completely absorbed into mucosa via a buccal delivery system and homeopathic effects might occur.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Skin Protection and Aging · Collagen: Extraction and Characterization · Dermatologic Treatments and Research
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Food and Nutrition, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Korea
Wooshin Labottach Co., Ltd., Seoul 08390, Korea
College of Pharmacy, Chosun University, Pilmundaero 309, Ding-gu, Gwangju 61452, Korea
References (49)
Show source-provided references
1. The collagen suprafamily: From bio-synthesis to advanced biomaterial development10.1002/adma.201801651 ↗
2. Collagen: A review on its sources and potential cosmetic applications
3. Extraction and characterization of collagen from buffalo skin for bio-medical applications10.13005/ojc/320336 ↗
4. Timing underpins the benefits associated with injectable collagen biomaterial therapy for the treatment of myocardial infarction10.1016/j.biomaterials.2014.11.004 ↗
5. Alves, A.L., Marques, A.L.P., Martins, E., Silva, T.H., and Reis, R.L. (2017). Cosmetic Potential of Marine Fish Skin Collagen. Cosmetics, 4.10.3390/cosmetics4040039 ↗
6. Guillerme, J.-B., Couteau, C., and Coiffard, L. (2017). Applications for Marine Resources in Cosmetics. Cosmetics, 4.10.3390/cosmetics4030035 ↗
7. Kinetics of moisturizing and firming effects of cosmetic formulations10.1111/j.1468-2494.2008.00436.x ↗
8. Hayashi, Y., Yamada, S., Ikeda, T., and Yanagiguchi, K. (2011). Marine Cosmeceuticals: Trends and Prospects, CRC Press.
9. Role of insoluhle and soluhle collagen as skin moisturizer
10. Fluhr, J.W., Elsner, P., Berardesca, E., and Maibach, H.I. (2004). Bioengineering of the Skin: Water and the Stratum Corneum, CRC Press.10.1201/b14244 ↗
11. Evaluation for collagen products for cosmetic application10.1111/j.1467-2494.2004.00245_2.x ↗
12. Development of Low-Molecular Weight Collagen Peptide Complex with Glycosaminoglycan Components10.1007/s10517-018-4229-0 ↗
13. Viscosity Effects of Hydrophilic Polymers on Transport of Collagen Hydrolysate Across Reconstructed Human Buccal Tissue10.1002/bkcs.11962 ↗
14. Physiology of Intestinal Absorption and Secretion10.1016/j.bpg.2016.02.007 ↗
15. Sustained buccal delivery of the hydrophobic drug denbufylline using physically cross-linked palmitoyl glycol chitosan hydrogels10.1016/S0939-6411(02)00118-2 ↗
16. A short review on “A novel approach in oral fast dissolving drug delivery system and their patents”
17. Combination of PLGA nanoparticles with mucoadhesive guar-gum films for buccal delivery of antihypertensive peptide10.1016/j.ijpharm.2018.05.051 ↗
18. Metia, P.K., and Bandyopadhyay, A.K. (2008). In vitro and in vivo evaluation of a novel mucoadhesive buccal oxytocin tablet prepared with Dillenia indica fruit mucilage. Die Pharm. Int. J. Pharm. Sci., 270–274.
19. In Vitro Evaluation of Various Buccal Permeation Enhancing Systems for PACAP (Pituitary Adenylate Cyclase-Activating Polypeptide)10.1007/s11095-005-7894-4 ↗
20. Transbuccal peptide delivery: Stability and in vitro per-meation studies on endomorphin-110.1016/S0168-3659(01)00246-2 ↗
21. Bolke, L., Schlippe, G., Gerß, J., and Voss, W. (2019). A Collagen Supplement Improves Skin Hydration, Elasticity, Roughness, and Density: Results of a Randomized, Placebo-Controlled, Blind Study. Nutrients, 11.10.3390/nu11102494 ↗
22. Clinical Evidence of the Anti-Aging Effects of a Collagen Peptide Nutraceutical Drink on the Skin
23. (2016). Anonymous International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use ICH Harmonised Guideline: Integrated addendum to ICH E6, Clinical Practice, European Medicines Agency. R1: Guideline for Good, E6(R2).
24. Benefits of using the cut-ometer to evaluate the effectiveness of skin treatments in plastic and maxillofacial surgery10.1016/j.revsto.2015.02.002 ↗
25. Expanded haemodialysis therapy of chronic haemodialysis patients prevents calcification and apoptosis of vascular smooth muscle cells in vitro10.1159/000484925 ↗
26. Ingestion of bioactive collagen hydrolysates enhance facial skin moisture and elasticity and reduce facial ageing signs in a randomised double-blind placebo-controlled clinical study10.1002/jsfa.7606 ↗
27. The effect of oral collagen peptide supplementation on skin moisture and the dermal collagen network: Evidence from an ex vivo model and randomized, placebo-controlled clinical trials10.1111/jocd.12174 ↗
28. Oral Intake of Specific Bioactive Collagen Peptides Reduces Skin Wrinkles and Increases Dermal Matrix Synthesis10.1159/000355523 ↗
29. Oral Supplementation of Specific Collagen Peptides Has Beneficial Effects on Human Skin Physiology: A Double-Blind, Placebo-Controlled Study10.1159/000351376 ↗
30. Collagen hydrolysate intake increases skin collagen expression and suppresses matrix metalloproteinase 2 activity10.1089/jmf.2010.0085 ↗
31. Identification of Food-Derived Collagen Peptides in Human Blood after Oral Ingestion of Gelatin Hydrolysates10.1021/jf050206p ↗
32. Hydroxypro-line-containing dipeptides and tripeptides quantified at high concentration in human blood after oral administration of gelatin hydrolysate10.3109/09637480903257711 ↗
33. Transdermal delivery of a pineal hormone: Melatonin via elastic liposomes10.1016/j.biomaterials.2006.01.060 ↗
34. Asai, T.T., Oikawa, F., Yoshikawa, K., Inoue, N., and Sato, K. (2019). Food-derived collagen peptides, prolyl-hydroxyproline (Pro–Hyp), and hydroxyprolyl-glycine (Hyp–Gly) enhance growth of primary cultured mouse skin fibroblast using fetal bovine serum free from hydroxyprolyl peptide. Int. J. Mol. Sci., 21.10.3390/ijms21010229 ↗
35. Effect of homeopathic Arnica montana on bruising in face-lifts: Results of a randomized, double-blind, placebo-controlled clinical trial10.1001/archfaci.8.1.54 ↗
36. Goldschmidt, M., and Clemow, J.B. (2021). Neuromodulators (Botox). Applied Head and Neck Anatomy for the Facial Cosmetic Surgeon, Springer.10.1007/978-3-030-57931-9_15 ↗
37. Platelets Amplify Inflammation in Arthritis via Collagen-Dependent Microparticle Production10.1126/science.1181928 ↗
38. Regulation of Joint Destruction and Inflammation by p53 in Collagen-Induced Arthritis10.1016/S0002-9440(10)64356-8 ↗
39. IL-1-Independent Role of IL-17 in Synovial Inflammation and Joint Destruction During Collagen-Induced Arthritis10.4049/jimmunol.167.2.1004 ↗
40. Late-onset migrated inflammatory granuloma after collagen-containing filler injection10.3341/jkos.2008.49.8.1330 ↗
41. Anti-inflammatory activity Effects of Mori Folium Water Extraction IL-1α, IL-6 and IL-10 on mouse macrophages
42. Molecular inflammation hypothesis of aging based on the anti-aging mechanism of calorie restriction10.1002/jemt.10203 ↗
43. Generation, toxicity and scavenging of ONOO−: Its involvement in the aging process
44. Peroxynitrite scavenging activity of Sabohwanin lipopolysaccha-ride-induced oxidatively-stressed mice
45. Phenolic Antioxidants Prevent Peroxynitrite-Derived Collagen Modification in Vitro10.1021/jf960865p ↗
46. Chronic hypoxia increases peroxynitrite, MMP9 expression, and collagen accumulation in fetal guinea pig hearts10.1038/pr.2011.10 ↗
47. Effect of nitric oxide and peroxynitrite on type I collagen synthesis in normal and scleroderma dermal fibroblasts10.1016/j.freeradbiomed.2007.04.017 ↗
48. Regulation of fibroblast-mediated collagen gel contraction by platelet-derived growth factor, interleukin-1 alpha and transforming growth factor-beta 110.1242/jcs.102.2.315 ↗
49. A fibronectin fragment induces type II collagen degradation by colla-genase through an interleukin-1—mediated pathway10.1002/1529-0131(200201)46:1<138::AID-ART10051>3.0.CO;2-K ↗