Extended Dual-Focus Microscopy for Ratiometric-Based 3D Movement Tracking
Source abstract
Abstract
Imaging the three-dimensional movement of small organelles in living cells can provide key information for the dynamics of drug delivery and virus transmission in biomedical disciplines. To stably monitor such intracellular motion using microscope, long depth of field along optical axis and accurate three-dimensional tracking are simultaneously required. In the present work, we suggest an extended dual-focus optics microscopy system by combining a bifocal plane imaging scheme and objective lens oscillation, which enables accurate localization for a long axial range. The proposed system exploits high-resolution functionality by concatenating partial calibration result acquired each axial imaging level, maintaining the practical advantages of ratiometric method.
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Research context
OpenAlex topics: Advanced Fluorescence Microscopy Techniques · Digital Holography and Microscopy · Optical Coherence Tomography Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Data Science Research Division, Information Technology Center, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8656, Japan
Department of Physics, Graduate School of Science, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan
Funding
Japan Society for the Promotion of Science
References (36)
Show source-provided references
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