PDMS-Based Microdevices for the Capture of MicroRNA Biomarkers
Source abstract
Abstract
The isolation and analysis of circulating biomarkers, the main concern of liquid biopsy, could greatly benefit from microfluidics. Microfluidics has indeed the huge potentiality to bring liquid biopsy into the clinical practice. Here, two polydimethylsiloxane (PDMS)-based microdevices are presented as valid tools for capturing microRNAs biomarkers from clinically-relevant samples. After an extensive study of functionalized polydimethylsiloxane (PDMS) properties in adsorbing/eluting microRNAs, the best conditions were transferred to the microdevices, which were thoroughly characterized. The channels morphology and chemical composition were measured, and parameters for the automation of measures were setup. The best working conditions were then used with microdevices, which were proven to capture microRNAs on all channel surfaces. Finally, microfluidic devices were successfully validated via real-time PCR for the detection of a pool of microRNAs related to non-small cell lung cancer, selected as proof-of-principle. The microfluidic approach described here will allow a step forward towards the realization of an efficient microdevice, possibly automated and integrated into a microfluidic lab-on-a-chip with high analytical potentialities.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Microfluidic and Capillary Electrophoresis Applications · Innovative Microfluidic and Catalytic Techniques Innovation · Microfluidic and Bio-sensing Technologies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Fondazione Bruno Kessler, Center for Materials and Microsystems, via Sommarive 18, I-38123 Povo (Trento), Italy
CNR—Consiglio Nazionale delle Ricerche, Istituto di Biofisica, via alla Cascata 56/C, I-38123 Povo (Trento), Italy
Department of Applied Science and Technology, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy
CNR-IMEM, Parco Area delle Scienze 37a, 43124 Parma, Italy
Istituto Italiano di Tecnologia, Center for Sustainable Future Technologies @ PoliTo, Corso Trento 21, 10129 Torino, Italy
Funding
Regione Piemonte
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