Ultra-Sensitive Hydrogen Peroxide Sensor Based on Peroxiredoxin and Fluorescence Resonance Energy Transfer
Source abstract
Abstract
In this paper, a fluorescence resonance energy transfer (FRET)-based sensor for ultra-sensitive detection of H2O2 was developed by utilizing the unique enzymatic properties of peroxiredoxin (Prx) to H2O2. Cyan and yellow fluorescent protein (CFP and YFP) were fused to Prx and mutant thioredoxin (mTrx), respectively. In the presence of H2O2, Prx was oxidized into covalent homodimer through disulfide bonds, which were further reduced by mTrx to form a stable mixed disulfide bond intermediate between CFP-Prx and mTrx-YFP, inducing FRET. A linear quantification range of 10–320 nM was obtained according to the applied protein concentrations and the detection limit (LOD) was determined to be as low as 4 nM. By the assistance of glucose oxidase to transform glucose into H2O2, the CFP-Prx/mTrx-YFP system (CPmTY) was further exploited for the detection of glucose in real sample with good performance, suggesting this CPmTY protein sensor is highly practical.
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Research context
OpenAlex topics: Redox biology and oxidative stress · Sulfur Compounds in Biology · Nanoplatforms for cancer theranostics
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
State Key Laboratory of Bioreactor Engineering, School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China
Department of Bioproducts and Biosystems Engineering, University of Minnesota, St Paul, MN 55108, USA
Funding
National Science Foundation of China · the Fundamental Research Funds for the Central Universities · the 111 Project · the Project Funded by the International S&T Innovation Cooperation Key Project · Shanghai Pujiang Program
References (48)
Show source-provided references
1. Whiteness Improvement of Citric Acid Crosslinked Cotton Fabrics: H2O2 Bleaching under Alkaline Condition10.1016/j.carbpol.2016.04.007 ↗
2. Bleaching of Commercial Pulps with H2O2 Catalyzed by Heteropolyacids10.1016/S0960-8524(98)00075-3 ↗
3. New Biocide Foam Containing Hydrogen Peroxide for the Decontamination of Vertical Surface Contaminated With Bacillus Thuringiensis Spores
4. Oriented Growth of Cross-Linked Metal-Organic Framework Film on Graphene Surface for Non-Enzymatic Electrochemical Sensor of Hydrogen Peroxide in Disinfectant10.1016/j.talanta.2018.05.078 ↗
5. Long-Distance Apoptotic Killing of Cells Is Mediated by Hydrogen Peroxide in a Mitochondrial ROS-Dependent Fashion10.1038/sj.cdd.4401685 ↗
6. Role of Mitochondrial ROS in the Brain: From Physiology to Neurodegeneration10.1002/1873-3468.12964 ↗
7. ROS Promotes Cancer Cell Survival through Calcium Signaling10.1016/j.ccell.2018.05.010 ↗
8. Hydrogen Peroxide-Triggered Gene Silencing in Mammalian Cells through Boronated Antisense Oligonucleotides10.1039/C7SC04318J ↗
9. ROS-Dependent Signal Transduction10.1016/j.ceb.2014.09.010 ↗
10. Aquaporin-3 Mediates Hydrogen Peroxide-Dependent Responses to Environmental Stress in Colonic Epithelia10.1073/pnas.1612921114 ↗
11. Dual Oxidases and Hydrogen Peroxide in a Complex Dialogue between Host Mucosae and Bacteria10.1016/j.molmed.2009.10.003 ↗
12. Hydrogen Peroxide Participates in Perception and Transduction of Cold Stress Signal in Synechocystis10.1093/pcp/pcy067 ↗
13. Oxidation of Phenolic Compounds Catalyzed by Immobilized Multi-Enzyme Systems with Integrated Hydrogen Peroxide Production10.1039/C3GC41456F ↗
14. Mechanism of Superoxide and Hydrogen Peroxide Formation by Fumarate Reductase, Succinate Dehydrogenase, and Aspartate Oxidase10.1074/jbc.M204958200 ↗
15. Review of Glucose Oxidases and Glucose Dehydrogenases: A Bird’s Eye View of Glucose Sensing Enzymes10.1177/193229681100500507 ↗
16. Hydrogen Peroxide Generation with Immobilized Glucose Oxidase for Textile Bleaching10.1016/S0168-1656(01)00386-8 ↗
17. Detection of Hydrogen Peroxide (H2O2 ) Using a Colorimetric Sensor Based on Cellulose Nanowhiskers and Silver Nanoparticles10.1016/j.carbpol.2019.02.053 ↗
18. A Dual-Signal Colorimetric and near-Infrared Fluorescence Probe for the Detection of Exogenous and Endogenous Hydrogen Peroxide in Living Cells10.1016/j.snb.2018.09.126 ↗
19. Catalase-Inorganic Hybrid Microflowers Modified Glassy Carbon Electrode for Amperometric Detection of Hydrogen Peroxide10.1016/j.matlet.2019.01.157 ↗
20. A Doubly-Quenched Fluorescent Probe for Low-Background Detection of Mitochondrial H2O210.1021/acs.analchem.9b01294 ↗
21. Recent Progress in the Development of Small-Molecule Fluorescent Probes for the Detection of Hydrogen Peroxide10.1016/j.trac.2019.06.031 ↗
22. FRET-Based Upconversion Nanoprobe Sensitized by Nd3+ for the Ratiometric Detection of Hydrogen Peroxide in Vivo10.1021/acsami.8b21549 ↗
23. Genetically Encoded Fluorescent Indicator for Intracellular Hydrogen Peroxide10.1038/nmeth866 ↗
24. HyPer-3: A Genetically Encoded H2O2 Probe with Improved Performance for Ratiometric and Fluorescence Lifetime Imaging10.1021/cb300625g ↗
25. Red Fluorescent Genetically Encoded Indicator for Intracellular Hydrogen Peroxide10.1038/ncomms6222 ↗
26. Proximity-Based Protein Thiol Oxidation by H2O2-Scavenging Peroxidases10.1074/jbc.M109.059246 ↗
27. Spatial and Temporal Analysis of NADPH Oxidase-Generated Hydrogen Peroxide Signals by Novel Fluorescent Reporter Proteins10.1089/ars.2012.4594 ↗
28. Peroxiredoxin Functions as a Peroxidase and a Regulator and Sensor of Local Peroxides10.1074/jbc.R111.283432 ↗
29. Glutathionylation of the Active Site Cysteines of Peroxiredoxin 2 and Recycling by Glutaredoxin10.1074/jbc.M115.692798 ↗
30. Real-Time Monitoring of Basal H2O2 Levels with Peroxiredoxin-Based Probes10.1038/nchembio.2067 ↗
31. Monitoring Thioredoxin Redox with a Genetically Encoded Red Fluorescent Biosensor10.1038/nchembio.2417 ↗
32. Structural Snapshots of Yeast Alkyl Hydroperoxide Reductase Ahp1 Peroxiredoxin Reveal a Novel Two-Cysteine Mechanism of Electron Transfer to Eliminate Reactive Oxygen Species10.1074/jbc.M112.357368 ↗
33. Active-Site Mutants Form Mixed Disulfide Dimers That Resemble Enzyme-Substrate Reaction Intermediates10.1016/j.jmb.2008.03.077 ↗
34. Thioredoxin Affinity Chromatography: A Useful Method for Further Understanding the Thioredoxin Network10.1093/jxb/eri170 ↗
35. Novel Peroxiredoxin-Based Sensor for Sensitive Detection of Hydrogen Peroxide10.1016/j.bbrc.2019.07.062 ↗
36. Colorimetric Detection of Hydrogen Peroxide and Glucose by Exploiting the Peroxidase-like Activity of Papain10.1039/C9RA03111A ↗
37. A Near-Infrared Fluorescent Probe for Detection of Exogenous and Endogenous Hydrogen Peroxide in Vivo10.1016/j.dyepig.2019.04.055 ↗
38. An Acetate-Based NIR Fluorescent Probe for Selectively Imaging of Hydrogen Peroxide in Living Cells and in Vivo10.1016/j.snb.2019.02.100 ↗
39. Turn-on Fluorescent Detection of Hydrogen Peroxide and Triacetone Triperoxide via Enhancing Interfacial Interactions of a Blended System10.1021/acs.analchem.9b01255 ↗
40. Colorimetric Hydrazine Detection and Fluorescent Hydrogen Peroxide Imaging by Using a Multifunctional Chemical Probe10.1016/j.aca.2018.11.039 ↗
41. Fluorescent Fe3O4 Quantum Dots for H2O2 Detection10.1021/acsanm.9b00071 ↗
42. Construction of Sponge-like Graphitic Carbon Nitride and Silver Oxide Nanocomposite Probe for Highly Sensitive and Selective Turn-off Fluorometric Detection of Hydrogen Peroxide10.1016/j.jcis.2019.09.109 ↗
43. Bifunctional Gold Nanoclusters Enable Ratiometric Fluorescence Nanosensing of Hydrogen Peroxide and Glucose10.1016/j.talanta.2019.01.087 ↗
44. Silver-Iron Hierarchical Microflowers for Highly Efficient H2O2 Nonenzymatic Amperometric Detection10.1021/acssuschemeng.8b06182 ↗
45. A Novel Catalase Mimicking Nanocomposite of Mn(II)-Poly-L-Histidine-Carboxylated Multi Walled Carbon Nanotubes and the Application to Hydrogen Peroxide Sensing10.1016/j.ab.2018.12.007 ↗
46. Ultralow-Platinum-Loading Nanocarbon Hybrids for Highly Sensitive Hydrogen Peroxide Detection10.1016/j.snb.2018.12.041 ↗
47. Real-Time Quantification of Hydrogen Peroxide Production in Living Cells Using NiCo2S4@CoS2 Heterostructure10.1016/j.snb.2019.02.015 ↗
48. Photoelectrochemical Biosensor Based on Co3O4 Nanoenzyme Coupled with PbS Quantum Dots for Hydrogen Peroxide Detection10.1021/acsanm.9b00165 ↗