Molecular Specific and Sensitive Detection of Pyrazinamide and Its Metabolite Pyrazinoic Acid by Means of Surface Enhanced Raman Spectroscopy Employing In Situ Prepared Colloids
Source abstract
Abstract
The prodrug pyrazinamide (PZA) is metabolized by the mycobacteria to pyrazinoic acid (POA), which is expelled into the extracellular environment. PZA resistance is highly associated to a lack of POA efflux. Thus, by detecting a reduction of the concentration of POA in the extracellular environment, by means of lab-on-a-chip (LoC)-SERS (surface-enhanced Raman spectroscopy), an alternative approach for the discrimination of PZA resistant mycobacteria is introduced. A droplet-based microfluidic SERS device has been employed to illustrate the potential of the LoC-SERS method for the discrimination of PZA resistant mycobacteria. The two analytes were detected discretely in aqueous solution with a limit of detection of 27 µm for PZA and 21 µm for POA. The simultaneous detection of PZA and POA in aqueous mixtures could be realized within a concentration range from 20 μm to 50 μm for PZA and from 50 μm to 80 μm for POA.
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Research context
OpenAlex topics: Innovative Microfluidic and Catalytic Techniques Innovation · Biosensors and Analytical Detection · Mycobacterium research and diagnosis
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Leibniz Institute of Photonic Technology (IPHT)—Member of the Research Alliance “Leibniz Health Technologies”, Albert-Einstein-Straße 9, 07745 Jena, Germany
Friedrich-Schiller-University Jena, Institute of Physical Chemistry and Abbe Center of Photonics, Helmholtzweg 4, 07743 Jena, Germany
InfectoGnostics Research Campus Jena, Centre for Applied Research, Philosophenweg 7, 07743 Jena, Germany
Laboratorio de Bioinformática y Biología Molecular, Facultad de Ciencias, Universidad Peruana Cayetano Heredia, Lima 31, Peru
Funding
Bundesministerium für Bildung und Forschung · ERANET LAC · CONCYTEC-FONDECYT-Peru · Wellcome Trust Intermediate Fellowship
References (56)
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