Assessment of Cytotoxicity of Magnesium Oxide and Magnesium Hydroxide Nanoparticles using the Electric Cell-Substrate Impedance Sensing
Source abstract
Abstract
Magnesium (Mg)-based alloys have the potential for bone repair due to their properties of biodegradation, biocompatibility, and structural stability, which can eliminate the requirement for a second surgery for the removal of the implant. Nevertheless, uncontrolled degradation rate and possible cytotoxicity of the corrosion products at the implant sites are known current challenges for clinical applications. In this study, we assessed in vitro cytotoxicity of different concentrations (0 to 50 mM) of possible corrosion products in the form of magnesium oxide (MgO) and magnesium hydroxide (Mg(OH)2) nanoparticles (NPs) in human fetal osteoblast (hFOB) 1.19 cells. We measured cell proliferation, adhesion, migration, and cytotoxicity using a real-time, label-free, non-invasive electric cell-substrate impedance sensing (ECIS) system. Our results suggest that 1 mM concentrations of MgO/Mg(OH)2 NPs are tolerable in hFOB 1.19 cells. Based on our findings, we propose the development of innovative biodegradable Mg-based alloys for further in vivo animal testing and clinical trials in orthopedics.
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Research context
OpenAlex topics: Magnesium Alloys: Properties and Applications · Bone Tissue Engineering Materials · Magnesium in Health and Disease
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Energy and Environmental Systems, North Carolina Agricultural and Technical State University, Greensboro, NC 27411, USA
Engineering Research Center for Revolutionized Metallic Biomaterials, North Carolina Agricultural and Technical State University, Greensboro, NC 27411, USA
Department of Chemical, Biological and Bioengineering, North Carolina Agricultural and Technical State University, Greensboro, NC 27411, USA
Department of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC 27411, USA
Department of Mechanical Engineering, North Carolina Agricultural and Technical State University, Greensboro, NC 27411, USA
Funding
NIH grant (GM113728), a ARO grant (74386), and a NSF grant (1649243)
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