Synthesis and Surface Modification of Nanostructured F-Doped ZnO: Toward a Transducer for Label-Free Optical Biosensing
Source abstract
Abstract
In this work, the surface of nanostructured fluorine-doped ZnO (nZnO·F) is functionalized with protein A (PrA), and used as a model biomolecule. The chemical procedure is characterized by several analytical techniques such as Fourier Transform Infrared Spectroscopy, water contact angle analysis, and fluorescence microscopy. The surface modification of nZnO·F by binding increasing concentrations of PrA is also investigated by two label-free optical techniques, i.e., the spectroscopic reflectometry and the steady-state photoluminescence. The results are compared with those obtained using undoped nZnO substrates in order to highlight the better performances of nZnO·F due to the fluorine doping. The results of this study pave the way for the design and realization of a ZnO-based nanostructured platform for label-free optical sensing.
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Research context
OpenAlex topics: Nanowire Synthesis and Applications · ZnO doping and properties · Analytical Chemistry and Sensors
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
National Research Council, Institute for Microelectronics and Microsystems, Unit of Naples, 80131 Naples, Italy
Department of Chemical Sciences, Federico II University of Naples, 80138 Naples, Italy
Department of Chemical, Materials and Production Engineering, Federico II University of Naples, 80138 Naples, Italy
Department of Physics, Federico II University of Naples, 80138 Naples, Italy
Funding
POR FESR Campania 2014/2020
References (23)
Show source-provided references
1. Nanomaterials for biosensing applications: A review10.3389/fchem.2014.00063 ↗
2. ZnO nanostructures based biosensors for cancer and infectious disease applications: Perspectives, prospects and promises10.1016/j.trac.2016.10.001 ↗
3. Biosensor technology for detecting biological warfare agents: Recent progress and future trends10.1016/j.aca.2005.12.020 ↗
4. Metal oxide modified ZnO nanomaterials for biosensor applications10.1186/s40580-018-0159-9 ↗
5. Aminosilane functionalizations of mesoporous oxidized silicon for oligonucleotide synthesis and detection
6. Glutamine-binding protein from escherichia coli specifically binds a wheat gliadin peptide allowing the design of a new porous silicon-based optical biosensor10.1021/pr0600226 ↗
7. Time-resolved sensing of chemical species in porous silicon optical microcavity10.1016/j.snb.2003.12.044 ↗
8. Porous silicon-based photonic biosensors: Current status and emerging applications
9. Ultraviolet-emitting ZnO nanowires synthesized by a physical vapor deposition approach10.1063/1.1342050 ↗
10. Excitonic emissions observed in ZnO single crystal nanorods10.1063/1.1544437 ↗
11. Nanostructured zinc oxide particles in chemically modified electrodes for biosensor applications10.1080/00032710701792612 ↗
12. ZnO nano-rods synthesized by nano-particle-assisted pulsed-laser deposition10.1007/s00339-004-2797-5 ↗
13. ZnO nanoneedles grown vertically on Si substrates by non-catalytic vapor-phase epitaxy10.1002/adma.200290015 ↗
14. Versatile synthesis of ZnO nanowires for quantitative optical sensing of molecular biorecognition10.1016/j.snb.2015.05.135 ↗
15. Morphological, structural, and charge transfer properties of F-doped ZnO: A spectroscopic investigation10.1021/acs.jpcc.7b04821 ↗
16. Hydrothermal synthesis and growth mechanisms of different ZnO nanostructures and their gas-sensing properties10.1007/s10854-014-2545-3 ↗
17. Label-free immunoassay for porcine circovirus type 2 based on excessively tilted fiber grating modified with staphylococcal protein A10.1016/j.bios.2016.07.100 ↗
18. In situ monitoring and optimization of CuAAC-mediated protein functionalization of biosurfaces10.1016/j.snb.2016.07.071 ↗
19. Rapid and label-free detection of protein a by aptamer-tethered porous silicon nanostructures10.1016/j.jbiotec.2017.01.005 ↗
20. Socrates, G. (2001). Infrared and Raman Characteristic Group Frequencies: Tables and Charts, Wiley and Sons. [3rd ed.].
21. A study of the interactions between an IgG-binding domain based on the B domain of staphylococcal protein A and rabbit IgG
22. Self-organization of gold nanoparticles on silanated surfaces10.3762/bjnano.6.242 ↗
23. Moretta, R., Terracciano, M., Rea, I., Casalino, M., Dardano, P., and De Stefano, L. (2017, January 11–18). Functionalization of macroporous silicon for optical detection of bacteria. Proceedings of the 19th Italian National Conference on Photonic Technologies Fotonica IET 2017, Padua, Italy.10.1049/cp.2017.0198 ↗