Successful Release of Voriconazole and Flavonoids from MAPLE Deposited Bioactive Surfaces
Source abstract
Abstract
We explored the potential of biomimetic thin films fabricated by means of matrix-assisted pulsed laser evaporation (MAPLE) for releasing combinations of active substances represented by flavonoids (quercetin dihydrate and resveratrol) and antifungal compounds (amphotericin B and voriconazole) embedded in a polyvinylpyrrolidone biopolymer; the antifungal activity of the film components was evaluated using in vitro microbiological assays. Thin films were deposited using a pulsed KrF* excimer laser source which were structurally characterized using atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR). High-quality thin films with chemical structures similar to dropcast ones were created using an optimum laser fluence of ~80 mJ/cm2. Bioactive substances were included within the polymer thin films using the MAPLE technique. The results of the in vitro microbiology assay, which utilized a modified disk diffusion approach and were performed using two fungal strains (Candida albicans American Type Culture Collection (ATCC) 90028 and Candida parapsilosis American Type Culture Collection (ATCC) 22019), revealed that voriconazole was released in an active form from the polyvinylpyrrolidone matrix. The results of this study show that the MAPLE-deposited bioactive thin films have a promising potential for use in designing combination products and devices, such as drug delivery devices, and medical device surfaces with antifungal activity.
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Research context
OpenAlex topics: Essential Oils and Antimicrobial Activity · Antifungal resistance and susceptibility · Laser Applications in Dentistry and Medicine
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Lasers Department, National Institute for Lasers, Plasma & Radiation Physics, P.O. Box MG-36, 077125 Bucharest-Magurele, Romania
Faculty of Physics, University of Bucharest, 050107 Bucharest, Romania
Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, 1–7 Polizu Street, 011061 Bucharest, Romania
Microbiology Immunology Department, Faculty of Biology and Research Institute of the University of Bucharest (ICUB), University of Bucharest, 77206 Bucharest, Romania
Department of Biomedical Engineering, University of North Carolina, Chapel Hill, NC 27599, USA
Department of Physics and Engineering Physics, Tulane University, New Orleans, LA 70118, USA
Funding
Ministry of Research and Innovation, CNCS - UEFISCDI
References (31)
Show source-provided references
1. Candida survival strategies10.1016/bs.aambs.2014.12.002 ↗
2. Microbiological, Clinical, and Surgical Features of Fungal Prosthetic Joint Infections: A Multi-Institutional Experience10.2106/JBJS.I.00574 ↗
3. Overview of Treatment Approaches for Fungal Infections10.1016/j.ccm.2017.04.003 ↗
4. Printing amphotericin B on microneedles using matrix-assisted pulsed laser evaporation10.18063/IJB.2017.02.004 ↗
5. Pharmacokinetic/pharmacodynamic analysis of voriconazole against Candida spp. and Aspergillus spp. in children, adolescents and adults by Monte Carlo simulation10.1016/j.ijantimicag.2016.02.016 ↗
6. Optimization of Voriconazole Therapy for the Treatment of Invasive Fungal Infections in Adults10.1002/cpt.1012 ↗
7. Candidiasis: Predisposing factors, prevention, diagnosis and alternative treatment10.1007/s11046-014-9749-1 ↗
8. Candidemia in the intensive care unit: Miles to go before we sleep10.1097/CCM.0b013e31820f710b ↗
9. Synergistic combinations of antifungals and anti-virulence agents to fight against Candida albicans10.1080/21505594.2015.1039885 ↗
10. Mendez-Vilas, A. (2011). Combination Therapy: Synergism between Natural Plant Extracts and Antibiotics against Infectious Diseases. Science against Microbial Pathogens: Communicating Current Research and Technological Advances (Microbiol Book Series), Formatex Research Center.
11. Voriconazole Is DeliveredFrom Antifungal-Loaded Bone Cement10.1007/s11999-012-2463-8 ↗
12. Are anidulafungin or voriconazole released from polymethylmethacrylate in vitro?10.1007/s11999-010-1643-7 ↗
13. In vitro evaluation of olive- and grape-based natural extracts as potential preservatives for food10.1016/j.ifset.2007.07.011 ↗
14. Synergistic effect of the flavonoids catechin, quercetin and epigallocatechin gallate with fluconazole induce apoptosis in Candida tropicalis resistant to fluconazole
15. Triggered Nanoparticles as Therapeutics10.1016/j.nantod.2013.07.004 ↗
16. Novel Laser-Based Deposition of Active Protein Thin Films10.1021/cr010428w ↗
17. McGill, R.A., and Chrisey, D.B. (2000). Method of Producing a Film Coating by Matrix Assisted Pulsed Laser Deposition. (No. 6,025,036), U.S. Patent.
18. Pulsed Laser Deposition of Biocompatible Polymers: A Comparative Study in Case of Pullulan10.1016/j.tsf.2003.11.145 ↗
19. Functionalized polyvinyl alcohol derivatives thin films for controlled drug release and targeting systems: MAPLE deposition and morphological, chemical and in vitro characterization10.1016/j.apsusc.2008.09.047 ↗
20. Magnetic core/shell nanoparticle thin films deposited by MAPLE: Investigation by chemical, morphological and in vitro biological assays10.1016/j.apsusc.2012.02.055 ↗
21. Vaseashta, A., Braman, E., and Susmann, P. (2012). Pulsed Laser Processing of Functionalized Polysaccharides for Controlled Release Drug Delivery Systems. Technological Innovations in Sensing and Detection of Chemical, Biological, Radiological, Nuclear Threats and Ecological Terrorism, Springer. Part 4.10.1007/978-94-007-2488-4 ↗
22. Antimicrobial oligo(p-phenylene-ethynylene) film deposited by resonant infrared matrix-assisted pulsed laser evaporation10.1016/j.colsurfb.2014.01.033 ↗
23. Antimicrobial and bacteria-releasing multifunctional surfaces: Oligo (p-phenylene-ethynylene)/poly (N-isopropylacrylamide) films deposited by RIR-MAPLE10.1016/j.colsurfb.2014.12.043 ↗
24. Deposition of antibacterial of poly(1,3-bis-(p-carboxyphenoxy propane)-co-(sebacic anhydride)) 20:80/gentamicin sulfate composite coatings by MAPLE10.1016/j.apsusc.2010.11.141 ↗
25. Antimicrobial activity of biopolymer–antibiotic thin films fabricated by advanced pulsed laser methods10.1016/j.apsusc.2013.01.062 ↗
26. Antimicrobial activity of biopolymeric thin films containing flavonoid natural compounds and silver nanoparticles fabricated by MAPLE: A comparative study10.1016/j.apsusc.2015.11.252 ↗
27. Microbial Colonization of Biopolymeric Thin Films Containing Natural Compounds and Antibiotics Fabricated by MAPLE10.1016/j.apsusc.2014.11.145 ↗
28. Binns, C. (2010). Introduction to Nanoscience and Nanotechnology, John Wiley & Sons Inc.10.1002/9780470618837 ↗
29. Review on Combinatorial Approach for Inhibiting Candida albicans Biofilm
30. Andrew, S., Hitchcock, C.A., and Dorr, P.K. (2000). Antifungal Compositions Comprising Voriconazole and Trovafloxacin or Prodrugs Thereof. (Patent EP0982031A2).
31. Synergy Between Polyvinylpyrrolidone-Coated Silver Nanoparticles and Azole Antifungal Against Drug-Resistant Candida albicans10.1166/jnn.2016.10934 ↗