Site-Specific Hydrocarbon-Degrading Bacteria Consortium Developed Using Functional and Genomic Analyses
Source abstract
Abstract
Bioaugmentation, defined as the strategic incorporation of specifically selected microbial biomass into contaminated environments, can significantly enhance the biodegradation of pollutants and is extensively employed in soil bioremediation efforts. A multistep screening process was applied to develop an autochthonous microbial consortium, including (i) hydrocarbonoclastic strain isolation from soil chronically contaminated with petroleum hydrocarbons, (ii) bacterial selection according to genomic and functional traits, and (iii) consortium validation in the native contaminated soil through microcosm experiments. The selection of strains with the ability to degrade alkanes and aromatic hydrocarbons on synthetic media was further supported by genomic analysis, delivering a consortium with complementary degradative properties. The outcomes of the microcosm experiments corroborated the efficacy of the selected indigenous consortium, demonstrating that the combination of Acinetobacter guillouiae, A. radioresistens, and Pseudomonas zarinae as an inoculum in the bioaugmentation strategy was successful in achieving the removal of up to 26% and 76% of linear and polycyclic aromatic hydrocarbons, respectively, thereby effectively addressing areas where natural attenuation was insufficient.
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Research context
OpenAlex topics: Microbial bioremediation and biosurfactants · Pesticide and Herbicide Environmental Studies · Enzyme-mediated dye degradation
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department for Innovation in Biological, Agro-Food and Forest Systems (DIBAF), University of Tuscia, Via S. Camillo De Lellis, 01100 Viterbo, Italy
References (60)
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