journal article·2025-12-11Open access

Characteristics of Functional Cookies with Added Cranberries (Vaccinium oxycoccos) in the Form of a Polyphenol Preparation and a Microencapsulated Preparation

Natalia Żurek, Ireneusz Tomasz Kapusta

Applied Sciences· Volume 15· Issue 24

Abstract

This study assessed the effect of adding a cranberry polyphenol preparation in powder and microcapsule form to cookies on their physicochemical properties, polyphenol composition, health-promoting activity, and biocompatibility with normal human colonocytes. Cranberry powder was obtained by purifying the polyphenolic compounds, while microcapsules were obtained by encapsulating the powder in a mixture of sodium alginate and soy protein isolate. Cookies were prepared with 0.5, 1, 3 and 5% microcapsules, and 0.04, 0.08, 0.23 and 0.38% powder. The study showed that physicochemical parameters such as moisture, spreadability index, baking loss, hardness, and color significantly depended on the type and amount of the additive. Higher total polyphenol content was observed for cookies with powder (13.22 mg/100 g; P0.38), analyzed by using ultra-performance liquid chromatography (UPLC). The addition of microencapsulated powder increased the degree of anthocyanin preservation by 57.9% (primarily cyanidin 3-O-glucoside). The highest antioxidant activity, measured by cation radical scavenging activity (ABTS), copper ion reduction (CUPRAC), superoxide radical (O2−) and hydroxyl radical (OH˙) scavenging capacity tests, was observed for cookies with a 0.38% addition of polyphenol powder. These cookies also demonstrated the highest antidiabetic activity (α-amylase and α-glucosidase inhibition of 40.23 and 15.79%, respectively). All tested cookies also demonstrated high biocompatibility with human colonocytes. These findings contribute to the development of innovative functional bakery products with stable anthocyanin forms.
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Research context

OpenAlex topics: Microencapsulation and Drying Processes · Phytochemicals and Antioxidant Activities · Tea Polyphenols and Effects

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Food Technology and Human Nutrition, Faculty of Technology and Life Sciences, University of Rzeszów, 4 Zelwerowicza St., 35-601 Rzeszów, Poland

References (43)

Show source-provided references
1. Nicolosi, A., Laganà, V.R., and Di Gregorio, D. (2023). Habits, Health and Environment in the Purchase of Bakery Products: Consumption Preferences and Sustainable Inclinations before and during COVID-19. Foods, 12.10.3390/foods12081661 ↗
2. Nutritional Profiling, Fiber Content and in Vitro Bioactivities of Wheat-Based Biscuits Formulated with Novel Ingredients10.1039/D4FO00204K ↗
3. The Use of Blackcurrant Pomace and Erythritol to Optimise the Functional Properties of Shortbread Cookies10.1038/s41598-024-54461-7 ↗
4. Valorisation of Apple Pomace for the Development of High-Fibre and Polyphenol-Rich Wheat Flour Cookies10.1038/s41598-024-77377-8 ↗
5. Encapsulation of Saffron and Sea Buckthorn Bioactives: Its Utilization for Development of Low Glycemic Baked Product for Growing Diabetic Population of the World10.1016/j.lwt.2021.111035 ↗
6. Pędziwiatr, D., Lamadrid, M.C., and Wojdyło, A. (2024). Cookies Fortified with Polyphenols Extracts: Impact on Phenolic Content, Antioxidant Activity, Inhibition of α-Amylase and α-Glucosidase Enzyme, Colour and Sensory Attractiveness. Antioxidants, 13.10.3390/antiox13091108 ↗
7. Obtaining Preparations with Increased Content of Bioactive Compounds from Eight Types of Berries10.3233/JBR-230020 ↗
8. American Cranberries and Health Benefits—an Evolving Story of 25 Years10.1002/jsfa.8882 ↗
9. Anthocyanin Stability and Degradation in Plants10.1080/15592324.2021.1987767 ↗
10. Microencapsulation of the Polyphenolic Fraction of Cranberry Fruit (Vaccinium oxycoccos) with Coatings Based on Protein Isolates: In Vitro Assessment of the Bioavailability of Polyphenolic Compounds10.1016/j.fbp.2025.08.009 ↗
11. Żurek, N., Świeca, M., Pawłowska, A., and Kapusta, I.T. (2024). Microencapsulation of Blueberry (Vaccinium myrtillus L.) Extracts via Ionotropic Gelation: In Vitro Assessment of Bioavailability of Phenolic Compounds and Their Activity against Colon Cancer Cells. Appl. Sci., 14.10.3390/app14177842 ↗
12. Effect of Black Soybean Flour Particle Size on the Nutritional, Texture and Physicochemical Characteristics of Cookies10.1016/j.lwt.2022.113649 ↗
13. Żurek, N., Pluta, S., Świeca, M., Potocki, L., Seliga, Ł., and Kapusta, I. (2025). Bioactive Compounds and In Vitro Health-Promoting Activity of the Fruit Skin and Flesh of Different Haskap Berry (Lonicera caerulea var. kamtschatica Sevast.) Cultivars. Int. J. Mol. Sci., 26.10.3390/ijms26146618 ↗
14. Effect of Fat and Sugar Replacement on Rheological, Textural and Nutritional Characteristics of Multigrain Cookies10.1007/s13197-020-04769-9 ↗
15. Development and Characterization of Functional Cookies Fortified with Microencapsulated Pomegranate Peel Extract Powder10.1007/s11694-024-03053-0 ↗
16. Aparna Optimization and Characterization of Functional Cookies with Addition of Tinospora Cordifolia as a Source of Bioactive Phenolic Antioxidants10.1016/j.lwt.2020.109639 ↗
17. High-Fiber Cookies with Bamboo Flour and Edible Flowers: Evaluation of Structural Properties, Phenolic Content, Antioxidant Activity and Nutritional Value10.1016/j.lwt.2025.117321 ↗
18. Salihu, S., Gashi, N., and Hasani, E. (2023). Effect of Plant Extracts Addition on the Physico-Chemical and Sensory Properties of Biscuits. Appl. Sci., 13.10.3390/app13179674 ↗
19. Effect of Orange-Fleshed Sweet Potato Flour Particle Size and Degree of Wheat Flour Substitution on Physical, Nutritional, Textural and Sensory Properties of Cookies10.1007/s00217-020-03672-z ↗
20. Bińkowska, W., Szpicer, A., Stelmasiak, A., Wojtasik-Kalinowska, I., and Półtorak, A. (2024). Utilization of Microencapsulated Polyphenols to Enhance the Bioactive Compound Content in Whole Grain Bread: Recipe Optimization. Appl. Sci., 14.10.3390/app142210156 ↗
21. Stabilization of Antioxidant Thyme-Leaves Extract (Thymus vulgaris) within Biopolymers and Its Application in Functional Bread Formulation10.1016/j.fufo.2024.100356 ↗
22. Incorporation of Wheat Malt into a Cookie Recipe and Its Effect on the Physicochemical Properties of the Corresponding Dough and Cookies10.1016/j.lwt.2019.108651 ↗
23. Analysis of Physical Properties in White and Whole Wheat Sliced Bread Using Digital Image Processing10.1007/s44187-025-00568-3 ↗
24. Francavilla, A., and Joye, I.J. (2022). Anthocyanin Content of Crackers and Bread Made with Purple and Blue Wheat Varieties. Molecules, 27.10.3390/molecules27217180 ↗
25. Phytochemical Compounds and Antioxidant Activity in Different Cultivars of Cranberry (Vaccinium macrocarpon L)10.1111/1750-3841.13924 ↗
26. Ma, H., Johnson, S., Liu, W., DaSilva, N., Meschwitz, S., Dain, J., and Seeram, N. (2018). Evaluation of Polyphenol Anthocyanin-Enriched Extracts of Blackberry, Black Raspberry, Blueberry, Cranberry, Red Raspberry, and Strawberry for Free Radical Scavenging, Reactive Carbonyl Species Trapping, Anti-Glycation, Anti-β-Amyloid Aggregation, and Microglial Neuroprotective Effects. Int. J. Mol. Sci., 19.10.3390/ijms19020461 ↗
27. Physical and Bioactive Properties of Muffins Enriched with Raspberry and Cranberry Pomace Powder: A Promising Application of Fruit By-Products Rich in Biocompounds10.1007/s11130-016-0539-4 ↗
28. Influence of Baking on Anthocyanin Content in Coloured-Grain Wheat Bread10.17221/210/2020-PSE ↗
29. Microencapsulation of Black Carrot Anthocyanins for Enhanced Thermal Stability
30. Dual-Layered pH-Sensitive Alginate/Chitosan/Kappa-Carrageenan Microbeads for Colon-Targeted Release of 5-Fluorouracil10.1016/j.ijbiomac.2019.03.225 ↗
31. Bińkowska, W., Szpicer, A., Stelmasiak, A., Wojtasik-Kalinowska, I., and Półtorak, A. (2024). Microencapsulation of Polyphenols and Their Application in Food Technology. Appl. Sci., 14.10.3390/app142411954 ↗
32. Tolve, R., Bianchi, F., Lomuscio, E., Sportiello, L., and Simonato, B. (2022). Current Advantages in the Application of Microencapsulation in Functional Bread Development. Foods, 12.10.3390/foods12010096 ↗
33. Microencapsulation as an Innovative Technique for the Preparation of High-Added Value Bakery Products—A Review10.35219/foodtechnology.2024.2.13 ↗
34. Schefer, S., Oest, M., and Rohn, S. (2021). Interactions between Phenolic Acids, Proteins, and Carbohydrates—Influence on Dough and Bread Properties. Foods, 10.10.3390/foods10112798 ↗
35. Munteanu, I.G., and Apetrei, C. (2021). Analytical Methods Used in Determining Antioxidant Activity: A Review. Int. J. Mol. Sci., 22.10.3390/ijms22073380 ↗
36. Rudrapal, M., Rakshit, G., Singh, R.P., Garse, S., Khan, J., and Chakraborty, S. (2024). Dietary Polyphenols: Review on Chemistry/Sources, Bioavailability/Metabolism, Antioxidant Effects, and Their Role in Disease Management. Antioxidants, 13.10.3390/antiox13040429 ↗
37. A Polyphenol-Rich Cranberry Extract Reverses Insulin Resistance and Hepatic Steatosis Independently of Body Weight Loss10.1016/j.molmet.2017.10.003 ↗
38. Feldman, F., Koudoufio, M., El-Jalbout, R., Sauvé, M.F., Ahmarani, L., Sané, A.T., Ould-Chikh, N.-E.-H., N’Timbane, T., Patey, N., and Desjardins, Y. (2022). Cranberry Proanthocyanidins as a Therapeutic Strategy to Curb Metabolic Syndrome and Fatty Liver-Associated Disorders. Antioxidants, 12.10.3390/antiox12010090 ↗
39. Li, X., Chen, W., Xia, J., Pan, D., and Sun, G. (2024). The Effects of Cranberry Consumption on Glycemic and Lipid Profiles in Humans: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. Nutrients, 16.10.3390/nu16060782 ↗
40. Modulation of the Glycaemic Index Value of Shortbread Cookies by the Use of Erythritol and Fruit Pomace10.1038/s41598-024-65108-y ↗
41. Metabolic Effects of the Natural Sweeteners Xylitol and Erythritol: A Comprehensive Review10.1080/10408398.2019.1623757 ↗
42. Miedes, D., Cilla, A., and Alegría, A. (2023). Chemopreventive Effect of an In Vitro Digested and Fermented Plant Sterol-Enriched Wholemeal Rye Bread in Colon Cancer Cells. Foods, 13.10.3390/foods13010112 ↗
43. Study on Biological Activity of Bread Enriched with Natural Polyphenols in Terms of Growth Inhibition of Tumor Intestine Cells10.1089/jmf.2019.0082 ↗