Pore Structure Analysis of Growing Media Using X-Ray Microcomputed Tomography

This study investigated the representative elementary volume (REV) for visible porosity in horticultural growing media (peat, commercial mixture, treated wood fibre/peat, pure wood fibre) using x-ray micro-computed tomography (µCT) with 2D and 3D image division, pore morphology, water retention curve (WRC), and saturated hydraulic conductivity (Ksat) via pore network modelling (PNM). Two sample sizes (10 × 10 cm, 3 × 3 cm, height × diameter) with resolutions of 46 and 15 µm were analysed. REV was assessed using deterministic (dREV) and statistical (sREV) criteria, evaluating the porosity and coefficient of variation across subvolumes. The results showed that 3D division of large samples achieved REV only for pure wood fibre (8000–10,000 µm), while 2D division met both criteria for all media. For small samples, 3D division achieved REV only for wood fibre/peat mixture, but 2D division succeeded for all media above 3000 µm. Pore analyses indicated that pure wood fibre had the largest, most connected pores, enhancing drainage, while peat showed complex, retentive structures. WRCs aligned well with lab data (R2 > 0.88). PNM Ksat estimates from small images were more accurate, with discrepancies (21–172%) due to segmentation artefacts. Future studies should incorporate permeability or tortuosity and explore multiscale imaging for improved hydrophysical predictions. This study also highlights advantages unique to X-ray µCT compared to standard laboratory methods, e.g., direct three-dimensional quantification of pore structure parameters and an image-based determination of the REV.