Functional Genomic and Phenotypic Analysis of Lactiplantibacillus pentosus P7 Isolated from Pickled Mustard Greens Reveals Capacity for Exopolysaccharide, B-Vitamin, and Lactic Acid Production
Source abstract
Abstract
Lactiplantibacillus pentosus is a lactic acid bacterium frequently detected in various fermented foods; however, the genomic traits related to its biotechnological potential have been underexplored. In this study, 34 catalase-negative isolates were obtained from pickled mustard greens, among which strain P7 exhibited the highest exopolysaccharide (EPS) yield (781.9 ± 14.7 mg/L) and was capable of growing in a chemically defined medium lacking riboflavin. Whole-genome sequencing revealed a 3,749,478 bp circular chromosome with 46.5% G + C content and 3389 protein-coding genes. A phylogenomic analysis identified P7 as L. pentosus. Functionally, 1 mg/mL EPS extracted from P7 demonstrated strong antioxidant activity, with DPPH and hydroxyl radical scavenging capacities of 89.8 ± 4.6% and 76.5 ± 9.5%, respectively. The use of 0.2 mg/mL EPS also protected Saccharomyces cerevisiae cells from oxidative stress. A comparative genomic analysis indicated the presence of nearly complete biosynthetic pathways for riboflavin, folate, and pyridoxine. High-performance liquid chromatography (HPLC) confirmed the production of 23.8 ± 0.4 µg/mL riboflavin, 36.6 ± 0.6 µg/mL folic acid, and 0.42 ± 0.02 µg/mL pyridoxine in the culture supernatant, which have not been previously reported. Additionally, strain P7 produced 91.2 ± 12.3 g/L of lactic acid after 24 h of incubation. These results support the potential of L. pentosus P7 as a candidate for industrial applications in the production of EPS, B-group vitamins, and lactic acid.
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Research context
OpenAlex topics: Probiotics and Fermented Foods · Polyamine Metabolism and Applications · Microbial Metabolic Engineering and Bioproduction
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Institute of Biology, Vietnam Academy of Science and Technology, Hanoi 100000, Vietnam
Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi 100000, Vietnam
Center for High Technology Research and Development, Vietnam Academy of Science and Technology, Hanoi 100000, Vietnam
Funding
Vietnam Academy of Science and Technology
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