Unconventional Material from In Vitro Plant Cell Cultures: Vitis labrusca var. Isabella Case Study
Source abstract
Abstract
Isabella, an ancient hybrid grape originating from Vitis labrusca and Vitis vinifera genotypes, is widely cultivated for various food products and is considered a superfood due to its nutritional profile and high polyphenol content. To overcome the unsustainability of intensive agriculture and establish a new route towards more sustainable and socially fair superfood production, this work validated the establishment of undifferentiated in vitro cultures of V. labrusca var. Isabella. Two callus cell lines have been obtained on two different solid media, exhibiting distinct morpho-chemical characteristics. The total phenolic content and antioxidant activity of the callus juices were statistically different in the two cell lines. The subsequent qualitative–quantitative LC-MS analysis revealed the presence of seven stilbenoid derivatives in one cell line and three in the other; likewise, the total stilbenoid content was statistically different between the two cell lines (5.76 and 23.24 µg/mL of juice in the two cell lines on the 28th day of growth). The Isabella cell cultures possess nutritionally valuable profiles. These results suggest that plant cell culture technology can be a sustainable and viable option for the production of complementary, added-value food materials.
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Research context
OpenAlex topics: Plant tissue culture and regeneration · Plant Pathogens and Fungal Diseases · Plant Reproductive Biology
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo, 5, 35131 Padua, Italy
Department of Molecular Medicine, University of Padova, Via Gabelli 63, 35121 Padova, Italy
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