The Effects of Anthyllis vulneraria Hydroalcoholic Leaf Extract as an Adjuvant in Wound Healing
Source abstract
Abstract
Anthyllis vulneraria is a traditional medicinal plant with confirmed anti-inflammatory properties, attributed to its high polyphenolic content. This study aimed to evaluate the wound-healing potential of A. vulneraria leaf extract in a rat burn model. Four groups of eight Wistar rats each received the following daily topical applications for 14 days: vehicle cream (negative control); silver sulfadiazine (positive control); or plant-based creams containing either 1 mg/cm2 or 2 mg/cm2 of polyphenols (experimental groups 1 and 2, respectively). On days 7 and 14, four animals per group were euthanized for histological and oxidative stress evaluations. LC-MS/MS analysis of the leaf extract identified hyperoside, ferulic acid, and p-coumaric acid as major constituents. Experimental group 1 showed significantly enhanced wound closure on days 5 and 7, while group 2 exhibited a significant effect on day 5. All oxidative stress markers, except catalase activity, differed significantly among the groups, with the most favorable results observed in group 2. IL-8 levels decreased after the extract treatment, while no significant microscopic changes were observed. These results indicate that A. vulneraria leaf extract may serve as a valuable adjuvant in burn wound healing.
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Research context
OpenAlex topics: Wound Healing and Treatments · Pomegranate: compositions and health benefits · Plant-derived Lignans Synthesis and Bioactivity
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Faculty of Medicine, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400349 Cluj-Napoca, Romania
Department of Pharmaceutical Botany, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 23 Gheorghe Marinescu Street, 400337 Cluj-Napoca, Romania
Department of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania
Physiology Department, Faculty of Medicine, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400126 Cluj-Napoca, Romania
Department 1, Faculty of Medical Assistance and Health Sciences, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400126 Cluj-Napoca, Romania
Department of Anatomy and Embriology, “Iuliu Hațieganu” University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania
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