journal article·2025-07-09Open access

Quercetin-Fortified Animal Forage from Onion Waste: A Zero-Waste Approach to Bioactive Feed Development

Janusz Wojtczak, Krystyna Szymandera-Buszka, Joanna Kobus-Cisowska, Kinga Stuper-Szablewska, Jarosław Jakubowicz, Grzegorz Fiutak, Joanna Zeyland, Maciej Jarzębski

Applied Sciences· Volume 15· Issue 14

Abstract

There is a high demand for the development of new carriers for pharmaceutical forms for human, veterinary, and animal-feeding use. One of the solutions might be bioactive compound-loading pellets for animal forage. The aim of the work was to assess the physical and sensory properties of forage with the addition of onion peel and off-spec onions as a source of quercetin. The feed was prepared using an expanding process (thermal–mechanical expanding process). Quercetin content was evaluated in raw onion and in final-product feed mixture samples (before and after expanding, and pelleting). The obtained feed was subjected to sensory analysis, testing for expanded pellet uniformity, water absorption index (WAI), the angle of a slide, and antioxidant activity. The results confirmed a high recovery of the quercetin after the expanding process (approximately 80%), and a significantly reduced intensity of onion odor, which was confirmed compared to the non-expanded onion, which is beneficial. Furthermore, digital and optical microscopy were applied for structure analysis. Microscopic imaging results confirmed that the onion structures were visible in the whole length of feed material and analyzed cross-sections. The results can be an introduction to further research on developing products that use the expanding and pelleting process to exploit the peel and off-spec onions, as well as other waste raw materials.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Garlic and Onion Studies · Pineapple and bromelain studies · Banana Cultivation and Research

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Animal Breeding and Product Quality Assessment, Faculty of Veterinary Medicine and Animal Science, Poznań University of Life Sciences, Słoneczna 1, 62-002 Złotniki, Poland

Department of Gastronomy Sciences and Functional Food, Poznań University of Life Sciences, Wojska Polskiego 28, 60-637 Poznan, Poland

Department of Chemistry, Poznań University of Life Sciences, Wojska Polskiego 75, 60-625 Poznan, Poland

Institute of Materials Science and Engineering, Poznan University of Technology, Jana Pawla II 24, 61-138 Poznan, Poland

Department of Biotechnology and General Technology of Food, Faculty of Food Technology, University of Agriculture in Krakow, Balicka 122, 30-149 Krakow, Poland

Department of Biochemistry and Biotechnology, Poznań University of Life Sciences, Dojazd Street 11, 60-632 Poznan, Poland

Department of Physics and Biophysics, Faculty of Food Science and Nutrition, Poznań University of Life Sciences, 60-637 Poznan, Poland

Funding

European Union under the “Cooperation” action of the Rural Development Programme for 2014–2020

References (74)

Show source-provided references
1. Wood, J.D. (2023). Meat composition and nutritional value. Lawrie’s Meat Science, Elsevier.10.1016/B978-0-323-85408-5.00012-1 ↗
2. Perspective: The Place of Pork Meat in Sustainable Healthy Diets10.1016/j.advnut.2024.100213 ↗
3. The quantitative and qualitative changes of thiamine in sterilized pork in the presence of selected technological additives
4. O’Connor, L.E., Herrick, K.A., Parsons, R., and Reedy, J. (2021). Heterogeneity in Meat Food Groups Can Meaningfully Alter Population-Level Intake Estimates of Red Meat and Poultry. Front. Nutr., 8.10.3389/fnut.2021.778369 ↗
5. Stadnik, J. (2024). Nutritional Value of Meat and Meat Products and Their Role in Human Health. Nutrients, 16.10.3390/nu16101446 ↗
6. Ren, H., Xiao, W., Qin, X., Cai, G., Chen, H., Hua, Z., Cheng, C., Li, X., Hua, W., and Xiao, H. (2020). Myostatin regulates fatty acid desaturation and fat deposition through MEF2C/miR222/SCD5 cascade in pigs. Commun. Biol., 3.10.1038/s42003-020-01348-8 ↗
7. Czech, A., Klimiuk, K., and Sembratowicz, I. (2023). Effect of the inclusion of extruded flaxseed in the diet of fattening pigs on lipid metabolism and tissue redox status. Sci. Rep., 13.10.1038/s41598-023-40378-0 ↗
8. Effects of dietary flaxseed oil with or without products with antioxidant properties on pig performance, carcass characteristics, meat quality and oxidative stability10.1071/AN21458 ↗
9. Effects of rice bran, flax seed, and sunflower seed on growth performance, carcass characteristics, fatty acid composition, free amino acid and peptide contents, and sensory evaluations of native Korean cattle (Hanwoo)10.5713/ajas.15.0264 ↗
10. Monitoring the oxidation of docosahexaenoic acid in lipids10.1007/s11745-005-1458-1 ↗
11. Review: In vivo and postmortem effects of feed antioxidants in livestock: A review of the implications on authorization of antioxidant feed additives10.1017/S1751731115002967 ↗
12. Ponnampalam, E.N., Kearns, M., Kiani, A., Santhiravel, S., Vahmani, P., Prache, S., Monahan, F.J., and Mapiye, C. (2024). Enrichment of ruminant meats with health enhancing fatty acids and antioxidants: Feed-based effects on nutritional value and human health aspects—Invited review. Front. Anim. Sci., 5.10.3389/fanim.2024.1329346 ↗
13. Antioxidant and anti-inflammatory activities of quercetin and its derivatives10.1016/j.jff.2017.10.047 ↗
14. Valorization of onion peel waste: From trash to treasure10.1016/j.chemosphere.2023.140178 ↗
15. Effect of cooking on yellow onion quercetin
16. Kumar, M., Barbhai, M.D., Hasan, M., Punia, S., Dhumal, S., Rais, N., Chandran, D., Pandiselvam, R., and Kothakota, A. (2022). Onion (Allium cepa L.) peels: A review on bioactive compounds and biomedical activities. Biomed. Pharmacother., 146.10.1016/j.biopha.2021.112498 ↗
17. Waste streams in onion production: Bioactive compounds, quercetin and use of antimicrobial and antioxidative properties10.1016/j.wasman.2021.03.033 ↗
18. Production of flavonol quercetin and fructooligosaccharides from onion (Allium cepa L.) waste: An environmental life cycle approach10.1016/j.cej.2019.123772 ↗
19. A Review: Onion (Allium cepa)
20. Harvesting the benefits of nutritional research to address global challenges in the 21st century10.1111/jwas.12948 ↗
21. A comparison of the effect of diet extrusion or screw-press pelleting on the digestibility of grain protein products when fed to rainbow trout (Oncorhynchus mykiss)10.1016/j.aquaculture.2010.12.025 ↗
22. Gumienna, M., Lasik-Kurdyś, M., Szymandera-Buszka, K., Górna-Szweda, B., Walkowiak-Tomczak, D., and Jędrusek-Golińska, A. (2025). Innovative Application of Fermented Red Bean Seeds in Constructing Foods with Increased Biological Activity. Foods, 14.10.3390/foods14010088 ↗
23. Feed Processing Using the Annular Gap Expander and Its Impact on Poultry Performance’
24. Pelleting of broiler diets: An overview with emphasis on pellet quality and nutritional value10.1016/j.anifeedsci.2012.10.011 ↗
25. Effect of Nutrient Management on Two Varieties (Hybrid and Local) of Maize in Western Inner Terai of Nepal10.3126/ijasbt.v8i2.29586 ↗
26. Effect of feed form and heat processing on the growth performance of growing and finishing pigs10.1016/j.livsci.2021.104430 ↗
27. Bastiaansen, T.M.M., de Vries, S., Martens, B.M.J., Benders, R.T., Vissers, E., Dijksman, J.A., Hendriks, W.H., Thomas, M., and Bosch, G. (2024). Identifying feed characteristics that affect the pellet manufacturing of livestock diets containing different coproducts. Clean. Circ. Bioeconomy, 7.10.1016/j.clcb.2024.100073 ↗
28. Glass transition temperature and its relevance in food processing10.1146/annurev.food.102308.124139 ↗
29. Evidence of animal productivity outcomes when fed diets including food waste: A systematic review of global primary data10.1016/j.resconrec.2024.107411 ↗
30. Fundamental factors in feed manufacturing: Towards a unifying conditioning/pelleting framework10.1016/j.anifeedsci.2020.114612 ↗
31. Response of non-enzymatic antioxidative mechanisms to stress caused by infection with Fusarium fungi and chemical protection in different wheat genotypes10.1080/02757540.2017.1381689 ↗
32. Przybylska-Balcerek, A., Szablewski, T., Szwajkowska-Michałek, L., Świerk, D., Cegielska-Radziejewska, R., Krejpcio, Z., Suchowilska, E., Tomczyk, Ł., and Stuper-Szablewska, K. (2021). Sambucus Nigra Extracts–Natural Antioxidants and Antimicrobial Compounds. Molecules, 26.10.3390/molecules26102910 ↗
33. Comparision of antioxidant activities of onion and garlic extracts by inhibition of lipid peroxidation and radical scavenging activity10.1016/S0308-8146(02)00476-4 ↗
34. Flavonoid content of several vegetables and their antioxidant activity10.1002/(SICI)1097-0010(200004)80:5<561::AID-JSFA574>3.0.CO;2-# ↗
35. Antioxidant activity an improved ABTS radical cation decolorization assay10.1016/S0891-5849(98)00315-3 ↗
36. (2016). Test Sieves—Technical Requirements and Testing—Part 1: Test Sieves of Metal Wire Cloth (Standard No. ISO 3310-1:2016).
37. Impact of extrusion processing on bioactive compound enriched plant-based extrudates: A comprehensive study and optimization using RSM and ANN-GA10.1016/j.fufo.2023.100286 ↗
38. Physical and chemical properties of agricultural wastes
39. Datta, A.K. (1994). Food Process Engineering: Heat and Mass Transfer. Reference Module in Food Science, Elsevier.10.1016/B978-0-08-100596-5.02880-8 ↗
40. (2009). Sensory Analysis—General Guidance for the Design of Test Rooms (Standard No. ISO 8589).
41. Variation of quercetin glycoside derivatives in three onion (Allium cepa L.) varieties10.1016/j.sjbs.2016.05.014 ↗
42. Qiao, L., Sun, Y., Chen, R., Fu, Y., Zhang, W., Li, X., Chen, J., Shen, Y., and Ye, X. (2014). Sonochemical effects on 14 flavonoids common in citrus: Relation to stability. PLoS ONE, 9.10.1371/journal.pone.0087766 ↗
43. Microbial Contamination and Decontamination of Onion and its Products10.1016/j.afres.2021.100032 ↗
44. Stability of quercetin derivatives in vacuum impregnated apple slices after drying (microwave vacuum drying, air drying, freeze drying) and storage10.1016/j.lwt.2013.11.021 ↗
45. Thermal Degradation of Onion Quercetin Glucosides under Roasting Conditions10.1021/jf063221i ↗
46. Protective effect of quercetin on pig intestinal integrity after transport stress is associated with regulation oxidative status and inflammation10.1292/jvms.16-0090 ↗
47. Influences of dietary flavonoid (quercetin) supplementation on growth performance and immune response of growing pigs challenged with Escherichia coli lipopolysaccharide10.5187/jast.2020.62.5.605 ↗
48. Preparation of core–shell hordein/pectin nanoparticles as quercetin delivery matrices: Physicochemical properties and colon-specific release analyses10.1016/j.foodres.2023.112971 ↗
49. Impact of binding interaction modes between whey protein concentrate and quercetin on protein structural and functional characteristics10.1016/j.foodhyd.2023.108787 ↗
50. Effect of pelleting and different feeding programs on growth performance, carcass yield, and nutrient digestibility in broiler chickens10.3382/ps/pez176 ↗
51. Norrapoke, T., and Pongjongmit, T. (2025). Effect of high-quality pellet feed level on voluntary feed intake, nutrient digestibility and rumen fermentation in beef cattle. Sci. Rep., 15.10.1038/s41598-025-96455-z ↗
52. Physical and chemical quality of forage feed pellets with different types of materials and compositions10.1088/1755-1315/951/1/012035 ↗
53. Celano, R., Docimo, T., Piccinelli, A.L., Gazzerro, P., Tucci, M., Di Sanzo, R., Carabetta, S., Campone, L., Russo, M., and Rastrelli, L. (2021). Onion peel: Turning a food waste into a resource. Antioxidants, 10.10.3390/antiox10020304 ↗
54. Infuence of processing on the antioxidant properties of fruit and vegetables10.1016/S0924-2244(99)00023-0 ↗
55. Thermal Processing Enhances the Nutritional Value of Tomatoes by Increasing Total Antioxidant Activity10.1021/jf0115589 ↗
56. Factors Influencing Food Powder Flowability10.3390/powders3010006 ↗
57. Water solubility index and water absorption index of extruded product from rice and carrot blend
58. Interlaboratory comparison of methods determining the botanical composition of animal feed10.5740/jaoacint.17-0150 ↗
59. Size and shape attributes of packaging remnants commonly detected in former food products10.1080/1828051X.2024.2348092 ↗
60. Bianchi, M.G., Casati, L., Sauro, G., Taurino, G., Griffini, E., Milani, C., Ventura, M., Bussolati, O., and Chiu, M. (2025). Biological Effects of Micro- / Nano-Plastics in Macrophages. Nanomaterials, 15.10.3390/nano15050394 ↗
61. Integrating micro- and nanowaste glass with waste foundry sand in ultra-high-performance concrete to enhance material performance and sustainability10.1515/rams-2024-0012 ↗
62. Identification and Quantification of Microplastics in the Marine Environment Using the Laser Direct Infrared (LDIR) Technique10.1021/acs.est.1c08870 ↗
63. Heme iron as potential iron fortifier for food application-characterization by material techniques10.1515/rams-2023-0128 ↗
64. Neder-Suárez, D., Amaya-Guerra, C.A., Quintero-Ramos, A., Pérez-Carrillo, E., Alanís-Guzmán, M.G.D.J., Báez-González, J.G., García-Díaz, C.L., Núñez-González, M.A., Lardizábal-Gutiérrez, D., and Jiménez-Castro, J.A. (2016). Physicochemical changes and resistant-starch content of extruded cornstarch with and without storage at refrigerator temperatures. Molecules, 21.10.3390/molecules21081064 ↗
65. The Psychophysics of Pouring, Spreading and in-Mouth Viscosity10.1111/j.1745-4603.1992.tb00528.x ↗
66. Effect of extrusion in the elaboration of an animal feed based on Moringa oleifera Lam and Zea mays as a partial substitute of fishmeal in the diet of the adult stage of Oreochromis niloticus
67. Development of extruded snacks using taro (Colocasia esculenta) and nixtamalized maize (Zea mays) flour blends10.1016/j.lwt.2010.06.036 ↗
68. Some Engineering Properties of Different Feed Pellets
69. A review on the angle of repose of granular materials10.1016/j.powtec.2018.02.003 ↗
70. Madrid, M.A., Fuentes, J.M., Ayuga, F., and Gallego, E. (2022). Determination of the Angle of Repose and Coefficient of Rolling Friction for Wood Pellets. Agronomy, 12.10.3390/agronomy12020424 ↗
71. Sweetness and texture perception in mixed pectin gels with 30% sugar and a designed rheology10.1016/j.lwt.2008.08.014 ↗
72. Jafari, S.M., and Malekjani, N. (2019). Transporting Operations of Food Materials Within Food Factories, Elsevier.
73. Effects of feed formulation on feed manufacturing and pellet quality characteristics of poultry diets10.1017/S004393391100002X ↗
74. Białowiec, A., Micuda, M., Szumny, A., Łyczko, J., and Koziel, J.A. (2019). The proof-of-the-concept of application of pelletization for mitigation of volatile organic compounds emissions from carbonized refuse-derived fuel. Materials, 12.10.3390/ma12101692 ↗