DART-HRMS for the Rapid Assessment of Bioactive Compounds in Ultrasound-Processed Rapeseed Meal By-Product
Source abstract
Abstract
In line with the recommended European policy for a zero-waste crop supply chain, a lab-pilot optimisation process to valorise the by-products of industrially produced rapeseed meal (RM) was performed. Three batches of RM were first processed into ethanol-wash solutes (EWS) and then optimised (OEWS) by an ultrasound-assisted (UA) treatment. After direct analysis in real time–high resolution mass spectrometry (DART-HRMS) analysis, data were processed applying a partial least square–discriminant analysis (PLS-DA), which retrieved the 15 most discriminative ions able to characterise the biochemical changes during the ethanol-washing and UA optimisation process. The metabolomic fingerprinting of EWS and OEWS generated an accurate and well-defined 3D spatial clusterisation based on a restricted pool of informative bioactive compounds. A significantly higher relative abundance of sinapic, azelaic, and vernolic acids and a lower incidence of the oleic and palmitic fatty acids were detected in OEWS. DART-HRMS generated a vast amount of biochemical information in one single run, also demonstrating that its association with an untargeted multivariate statistical approach would be a valuable tool for revealing specific functional biomarkers. This would eventually enhance the circular and effective use of rapeseed residuals coming from this plant’s oilseed industry.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Metabolomics and Mass Spectrometry Studies · Meat and Animal Product Quality · Advanced Chemical Sensor Technologies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Agronomy, Food, Natural Resources, Animals, and Environment-DAFNAE, Padova University, Viale dell’Università, 16, 35020 Legnaro, Italy
Experimental Chemistry Laboratory, Istituto Zooprofilattico Sperimentale delle Venezie, Viale Fiume, 78, 36100 Vicenza, Italy
Department of Microbiology and Biotechnology, University of Food Technologies, 26 Maritza Blvd., 4002 Plovdiv, Bulgaria
Department of Biochemistry and Nutrition, University of Food Technologies, 26 Maritza Blvd., 4002 Plovdiv, Bulgaria
Department of Analytical Chemistry and Physical Chemistry, University of Food Technologies, 26 Maritza Blvd., 4002 Plovdiv, Bulgaria
Department of Animal Medicine, Production and Health, Padova University, Viale dell’Università, 16, 35020 Legnaro, Italy
Funding
Interconnected Nord-Est Innovation Ecosystem (iNEST) from the European Union Next GenerationEU · Bulgarian National Science Fund
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