Optimization of Ethanol Concentration and Wetting Time for Industrial-Scale Production of Ipomoea batatas L. Leaf Extract
Source abstract
Abstract
Extraction is one of the most important phases in the food, pharmaceutical, and nutraceutical industries, as it enables the isolation of valuable compounds from raw materials. Ipomoea batatas L. leaf extract has anti-diabetic qualities due to anthocyanidins, flavonols, flavanones, and phenolic acids. The goal of this study is to maximize extraction on a production scale with total flavonoids and fingerprint profiles that closely resemble standardized extracts. In this study, extraction was performed using the percolator method with optimization parameters, including ethanol concentration (40, 50, 60, and 70%) and wetting time (0.5, 3, and 24 h). Quality control in extraction was assessed through the total flavonoids and fingerprint analysis. TLC was used to determine the fingerprints of Ipomoea batatas L. leaf extract, followed by multivariate analysis. Using 60% ethanol and 3 h of wetting time produced total flavonoids of 19.86 ± 0.2 mg quercetin/g and a fingerprint close to the control with a similarity of 94.87%. Ethanol concentration and wetting time are critical parameters in Ipomoea batatas L. extraction. Quality control through total flavonoid determination and fingerprint analysis during the extraction process provides a standardized approach to maintain the quality, safety, and efficacy of Ipomoea batatas L. natural products.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Microbial Metabolites in Food Biotechnology · Polysaccharides Composition and Applications · Food composition and properties
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Doctoral Program of Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang 45363, Indonesia
Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang 45363, Indonesia
Pharmacy Department, Faculty of Mathematics and Natural Science, Udayana University, Kampus Bukit Jimbaran, Bali 80361, Indonesia
Institute of Biological Sciences, Faculty of Science, Universiti Malaya, Kuala Lumpur 50603, Malaysia
Research Collaboration Center for Radiopharmaceuticals Theranostic, National Research and Innovation Agency (BRIN), Sumedang 45363, Indonesia
Funding
Ministry of Education and Culture of Republic of Indonesia · Universitas Padjadjaran
References (52)
Show source-provided references
1. Discovery of processing-associated Q-marker of carbonized traditional Chinese medicine: An integrated strategy of metabolomics, systems pharmacology and in vivo high-throughput screening model10.1016/j.phymed.2022.154152 ↗
2. Current trends in extraction of plant bioactive molecules valuable for food use
3. WHO (2017). Guidelines on Good Herbal Processing, Annex 1 WHO Guidelines on Good Herbal Processing Practices for Herbal Medicines, WHO.
4. Different extraction methods for phenolic and volatile compounds recovery from Elsholtzia ciliata fresh and dried herbal materials10.1016/j.indcrop.2018.04.069 ↗
5. Wilson, J., Simpson, T., and Spelman, K. (2022). Total cannabidiol (CBD) concentrations and yields from traditional extraction methods: Percolation vs. maceration. Front. Pharmacol., 13.10.3389/fphar.2022.886993 ↗
6. Free radical scavenging and anti-acne activities of mangosteen fruit rind extracts prepared by different extraction methods10.3109/13880200903062671 ↗
7. Uhlenbrock, L., Sixt, M., Tegtmeier, M., Schulz, H., Hagels, H., Ditz, R., and Strube, J. (2018). Natural products extraction of the future-sustainable manufacturing solutions for societal needs. Processes, 6.10.3390/pr6100177 ↗
8. Determination of flavonols in green and black tea leaves and green tea infusions by high-performance liquid chromatography10.1016/S0963-9969(00)00156-3 ↗
9. Effects of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin-Ciocalteu methods10.1016/j.foodchem.2005.08.034 ↗
10. Optimization of conditions for the extraction of antioxidants from leaves of Syzygium cumini L. using different solvents10.1007/s12161-011-9242-0 ↗
11. Upscaling the Cymbopogon citratus (lemongrass) extraction process to obtain optimum alpha-glucosidase inhibitor (AGI) levels10.14716/ijtech.v11i3.3688 ↗
12. European Medicines Agency (EMA) (2024, September 13). Specifications: Test Procedures and Acceptance Criteria for Herbal Substances, Herbal Preparations and Herbal Medicinal Products/Traditional Herbal Medicinal Products. Available online: https://www.ema.europa.eu/en/specifications-test-procedures-acceptance-criteria-herbal-substances-herbal-preparations-herbal.
13. Food and Drug Administration (2024, October 15). Botanical Drug Development—Guidance for Industry, Available online: https://www.fda.gov/downloads/Drugs/Guidances/UCM458484.pdf.
14. Herbal drugs: Standards and regulation10.1016/j.fitote.2010.02.001 ↗
15. Modern HPTLC—A perfect tool for quality control of herbals and their preparations10.1093/jaoac/93.5.1399 ↗
16. Identification and quantification of phenolic compounds responsible for the antioxidant activity of sweet potatoes with different flesh colours using high performance thin layer chromatography (HPTLC)10.1016/j.jfca.2016.04.009 ↗
17. Determination of TLC fingerprint biomarker of Ipomoea batatas (L.) Lam leaves extracted with ethanol and its potential as antihyperglycemic agent10.3897/pharmacia.68.e71334 ↗
18. Study on GC-MS fingerprint of petroleum ether fraction of Shenqi Jiangtang Granules10.1016/j.dcmed.2021.03.004 ↗
19. Phytochemical fingerprint and chemometrics for natural food preparation pattern recognition: An innovative technique in food supplement quality control10.1007/s13197-015-2115-6 ↗
20. Quantitative and HPLC fingerprint analysis combined with chemometrics for quality evaluation of Codonopsis Radix processed with different methods
21. CIP (2019). Discovery to Impact, International Potato Center.
22. Antioxidant activities and polyphenols of sweet potato (Ipomoea batatas L.) leaves extracted with solvents of various polarities10.1016/j.fbio.2016.04.004 ↗
23. Krochmal-Marczak, B., Cebulak, T., Kapusta, I., Oszmiański, J., Kaszuba, J., and Zurek, N. (2020). The content of phenolic acids and flavonols in the leaves of nine varieties of sweet potatoes (Ipomoea batatas L.) depending on their development, grown in central Europe. Molecules, 25.10.3390/molecules25153473 ↗
24. Arisanti, C.I.S., Wirasuta, I.M.A.G., Musfiroh, I., Ikram, E.H.K., and Muchtaridi, M. (2023). Mechanism of anti-diabetic activity from sweet potato (Ipomoea batatas): A systematic review. Foods, 12.10.3390/foods12142810 ↗
25. The Potential of the flavonoid content of Ipomoea batatas L. as an alternative analog GLP-1 for diabetes type 2 treatment—Systematic Review10.3390/metabo14010029 ↗
26. Kemenkes, R.I. (2017). Farmakope Herbal Indonesia Edisi II.
27. Authentication of Piper betle L. folium and quantification of their antifungal-activity10.1016/j.jtcme.2016.08.006 ↗
28. Modern TLC: A key technique for identification and quality control of botanicals and dietary supplements10.1556/JPC.18.2005.1.6 ↗
29. Wirasuta, I.M.A.G., and Arisanti, C.I.S. (2024). Metoda Pembuatan Ekstrak Terstandar Daun Ubi Jalar (Ipomoea batatas) Sebagai Anti. (IDS000008593), Diabetes. Patent.
30. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica
31. Optimization of the extraction process of Phyllanthus niruri L.10.15343/0104-7809.202044134143 ↗
32. The optimal extraction parameters and anti-diabetic activity of flavonoids from Ipomoea batatas leaf
33. Dietary sweet potato (Ipomoea batatas L.) leaf extract attenuates hyperglycaemia by enhancing the secretion of glucagon-like peptide-1 (GLP-1)10.1039/C4FO00032C ↗
34. Optimization of flavonoid extraction from Passiflora quadrangularis leaves with sedative activity and evaluation of its stability under stress conditions10.1016/j.bjp.2018.06.005 ↗
35. Effects of extraction variables on pharmacological activities of vine tea extract (Ampelopsis grossedentata)10.3923/ijp.2018.495.505 ↗
36. Flavonoids: An overview10.1017/jns.2016.41 ↗
37. FT-IR and FT-Raman fingerprints of flavonoids—A review10.1016/j.foodchem.2022.133430 ↗
38. Pattern recognition methods and multivariate image analysis in HPTLC fingerprinting of propolis extracts10.1002/cem.2592 ↗
39. Marby, M.B., Markham, T.J., and Thomas, K.R. (1970). The Systematic Identification of Flavonoids, Springer. [1st ed.].10.1007/978-3-642-88458-0 ↗
40. A comparative metabolomics study of flavonoids in sweet potato with different flesh colors (Ipomoea batatas (L.) Lam)10.1016/j.foodchem.2018.03.125 ↗
41. Luo, D., Mu, T., and Sun, H. (2021). Profiling of phenolic acids and flavonoids in sweet potato (Ipomoea batatas L.) leaves and evaluation of their anti-oxidant and hypoglycemic activities. Food Biosci., 39.10.1016/j.fbio.2020.100801 ↗
42. Application of chemometrics in authentication of herbal medicines: A review10.1002/pca.2378 ↗
43. TLC fingerprinting and pattern recognition methods in the assessment of authenticity of poplar-type propolis10.1093/chromsci/bmw024 ↗
44. Comparative study of the alignment method on experimental and simulated chromatographic data10.1002/jssc.201400700 ↗
45. Warping methods for spectroscopic and chromatographic signal alignment: A tutorial10.1016/j.aca.2013.03.048 ↗
46. Development and application of HPTLC method for estimation of Rivaroxaban and Aspirin in bulk drug and in-house tablet form10.25135/jcm.78.2211.2636 ↗
47. HPTLC finger print analysis of phytophenols of Paederia foetida under different extraction regimen
48. Pressure and temperature effects on degradation kinetics and storage stability of total anthocyanins in blueberry juice10.1021/jf1015347 ↗
49. Optimization of solvent extraction of antioxidants from Eucalyptus globulus leaves by response surface methodology: Characterization and assessment of their bioactive properties10.1016/j.indcrop.2017.07.014 ↗
50. Characterization and quantification by LC-MS/MS of the chemical components of the heating products of the flavonoids extract in Pollen typhae for transformation rule exploration10.3390/molecules201018352 ↗
51. Flavones and flavone synthases10.1016/j.phytochem.2005.07.013 ↗
52. Ayeleso, T., Ramachela, K., and Mukwevho, E. (2018). Aqueous-methanol extracts of orange-fleshed sweet potato (Ipomoea batatas) ameliorate oxidative stress and modulate type 2 diabetes associated genes in insulin resistant C2C12 cells. Molecules, 23.10.3390/molecules23082058 ↗