Constituents from Ageratina pichinchensis and Their Inhibitory Effect on Nitric Oxide Production
Source abstract
Abstract
In this study, we report on the isolation, purification, and anti-inflammatory evaluation of compounds from the plant species Ageratina pichinchensis. Using open-column chromatography, 11 known compounds were purified, which chemical structures were elucidated by nuclear magnetic resonance techniques (1D and 2D). All compounds were evaluated in an in vitro model of RAW 264.7 mouse macrophage cells, measuring the nitric oxide inhibition to determine the anti-inflammatory effect. The compound betuletol 3-O-β-glucoside (11) inhibited nitric oxide with a half-maximal inhibitory concentration (IC50) of 75.08 ± 3.07% at 75 µM; additionally, it inhibited the secretion of interleukin 6 (IL-6) and activation of the nuclear factor (NF-kβ). These results suggest that the anti-inflammatory effect attributed to A. pichinchensis species is promoted by compound 11, which could be considered a potential anti-inflammatory agent by suppressing the expression of NF-kβ target genes, such as those involved in the proinflammatory pathway and inducible nitric oxide synthase (iNOS).
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Research context
OpenAlex topics: Fungal Biology and Applications · Sesquiterpenes and Asteraceae Studies · Seaweed-derived Bioactive Compounds
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Biotechnology Autonomous Metropolitan, University-Iztapalapa Campus, Av. Ferrocarril de San Rafael Atlixco 186, Col. Leyes de Reforma 1ª, Sección, Alcaldía Iztapalapa, México City 09310, CDMX, Mexico
Chemical Research Center-IICBA, Autonomous University of the State of Morelos, Av. Universidad 1001, Chamilpa, Cuernavaca 62209, Morelos, Mexico
Department of Wood, Pulp and Paper, University Center of Exact Sciences and Engineering, University of Guadalajara, Km. 15.5, Guadalajara-Nogales, Col. Las Agujas, Zapopan 45200, Jalisco, Mexico
Faculty of Pharmacy, Autonomous University of the State of Morelos, Av. Universidad 1001, Chamilpa, Cuernavaca 62209, Morelos, Mexico
Faculty of Medicine, Autonomous University of the State of Morelos, Leñeros s/n, Col. Los Volcanes, Cuernavaca 62359, Morelos, Mexico
Funding
Conahcyt
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