The Effect of Phosphorus Fertilization on Transcriptome Expression Profile during Lentil Pod and Seed Development
Source abstract
Abstract
Seed coat hardness and water permeability, which are determined by the accumulation of tannins through the phenylpropanoid pathway in the seed, are important lentil quality characteristics. The impact of seeds’ developmental stage and phosphorus (P) fertilization levels on tannin accumulation is still under research. Through RNA sequencing, this study explored the effect of three P treatments (P0, 6 mg kg−1; P1, 15 mg kg−1; and P2, 21 mg kg−1) and three seed maturity stages (S1, immature 2 mm seed in a flat pod; S2, fully developed seed within the pod; and S3, mature seed at the beginning of the pod’s discoloration) on lentil gene expression. The key findings highlighted a significant influence of the seed maturity stage on phenylpropanoid genes, with S1 displaying the highest expression levels, and on phosphorus-related Gene Ontology (GO) terms that presented the highest number of downregulated genes in the S3 to S1 comparison. P exhibited a targeted effect on the flavanone 3-hydroxylase (F3H) and flavonol synthase (FLS) genes and specific gene clusters, as shown by the differential gene expression analysis. This study investigates the molecular mechanisms related to phosphorus fertilization and seed maturity stages that influence tannin accumulation, offering valuable information for the enhancement of lentil product quality through breeding programs.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Genetic and Environmental Crop Studies · Legume Nitrogen Fixing Symbiosis · Plant Disease Resistance and Genetics
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
Department of Molecular Biology and Genetics, School of Life Sciences, Democritus University of Thrace, 68100 Alexandroupolis, Greece
Institute of Applied Biosciences, Center for Research and Technology Hellas, 57001 Thermi, Greece
Biology Department, SUNY College at Old Westbury, New York, NY 11731, USA
Institute of Plant Breeding & Genetic Resources, Hellenic Agricultural Organization-DEMETER, 57001 Thermi, Greece
Funding
uropean Union and Greek national funds through the Operational Program Competitiveness, Entrepreneurship and Innovation
References (61)
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