A High-Throughput In Vitro Radiobiology Platform for Megavoltage Photon Linear Accelerator Studies
Source abstract
Abstract
We designed and developed a multiwell tissue culture plate irradiation setup, and intensity modulated radiotherapy plans were generated for 96-, 24-, and 6-well tissue culture plates. We demonstrated concordance between planned and measured/imaged radiation dose profiles using radiochromic film, a 2D ion chamber array, and an electronic portal-imaging device. Cell viability, clonogenic potential, and γ-H2AX foci analyses showed no significant differences between intensity-modulated radiotherapy and open-field, homogeneous irradiations. This novel platform may help to expedite radiobiology experiments within a clinical environment and may be used for wide-ranging ex vivo radiobiology applications.
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Research context
OpenAlex topics: Advanced Radiotherapy Techniques · Radiation Therapy and Dosimetry · 3D Printing in Biomedical Research
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Clinical and Experimental Medicine, Faculty of Health and Medical Sciences, University of Surrey, Guildford GU2 7WG, UK
Department of Oncology, St Luke’s Cancer Centre, Royal Surrey County Hospital, Guildford GU2 7XX, UK
Department of Medical Physics, St Luke’s Cancer Centre, Royal Surrey County Hospital, Guildford GU2 7XX, UK
GenesisCare, Cromwell Hospital, London SW5 0TU, UK
Department of Medical Physics and Biomedical Engineering, University College London, London WC1E 6BT, UK
Funding
St Luke’s Cancer Centre Fund, Guildford, UK.
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